Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,259,683	G→A	5.7%	intergenic (‑34/+38)	tdcG ← / ← tdcF	L‑serine dehydratase 3, anaerobic/putative reactive intermediate deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,259,683	0	G	A	5.7%	233.0 / 2.9	70	intergenic (‑34/+38)	tdcG/tdcF	L‑serine dehydratase 3, anaerobic/putative reactive intermediate deaminase
Reads supporting (aligned to +/- strand):  ref base G (40/26);  new base A (2/2);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCGAAGA  >  NC_000913/3259542‑3259830                                                                                                                                              |                                                                                                                                                    gCTACTTTCCAGCCGATCAATAAAACTTTATCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCgg                                                                                                                                                >  1:201981/1‑147 (MQ=255)  cTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGaa                                                                                                                                              >  3:171271/1‑148 (MQ=255)   tACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAAc                                                                                                                                             >  7:157155/1‑148 (MQ=255)   tACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAAc                                                                                                                                             >  7:151812/1‑148 (MQ=255)            gCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCGATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTAc                                                                                                                                     >  2:145016/1‑147 (MQ=255)            gCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACg                                                                                                                                    <  6:362640/148‑1 (MQ=255)            gCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAAAGACCTTAc                                                                                                                                     >  1:42264/1‑147 (MQ=255)             ccGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCGATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGa                                                                                                                                   <  1:145016/148‑1 (MQ=255)             cagATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATATTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGa                                                                                                                                   <  5:267454/146‑1 (MQ=255)                 tCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGATTGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGT‑GAGCGGAACGACCTTACGACCGTc                                                                                                                              >  8:126146/1‑148 (MQ=255)                     tAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCg                                                                                                                           <  1:270263/148‑1 (MQ=255)                      aaaaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGc                                                                                                                          >  2:296094/1‑148 (MQ=255)                       aaaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCt                                                                                                                         >  3:96935/1‑148 (MQ=255)                         aCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTgg                                                                                                                                                     >  1:3245/1‑118 (MQ=255)                         aCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTgg                                                                                                                                                     <  2:3245/118‑1 (MQ=255)                                 cGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGT‑GAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCttt                                                                                                              <  7:126146/148‑1 (MQ=255)                                  ggCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCttt                                                                                                              <  4:171271/148‑1 (MQ=255)                                         attGGCCCCACTGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCAc                                                                                                        <  7:280196/147‑1 (MQ=255)                                             gCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   <  2:369329/148‑1 (MQ=255)                                                 cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   >  5:262943/1‑144 (MQ=255)                                                 cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   <  6:262943/144‑1 (MQ=255)                                                 cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTa                                                                                                  >  4:208041/1‑145 (MQ=255)                                                 cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTa                                                                                                  <  3:208041/145‑1 (MQ=255)                                                   cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCg                                                                                             <  4:96935/148‑1 (MQ=255)                                                    ggTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGa                                                                                            >  3:25311/1‑148 (MQ=255)                                                         gCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGc                                                                                                          >  8:185876/1‑129 (MQ=255)                                                         gCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGc                                                                                                          <  7:185876/129‑1 (MQ=255)                                                         gCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCt                                                                                       >  3:204865/1‑148 (MQ=255)                                                           gAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGTGATGGCtt                                                                                      >  2:300717/1‑147 (MQ=255)                                                                 gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTc                                                                               <  4:25311/148‑1 (MQ=255)                                                                     gACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGTACTACGTACTGCGATGGCTTCAATTTCCAGc                                                                           >  6:279432/1‑148 (MQ=255)                                                                          aTCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   >  2:97229/1‑119 (MQ=255)                                                                          aTCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   <  1:97229/119‑1 (MQ=255)                                                                            cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGCCCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACAt                                                                    >  5:361692/1‑148 (MQ=255)                                                                            cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGa                                                                                            >  1:267936/1‑124 (MQ=255)                                                                            cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGa                                                                                            <  2:267936/124‑1 (MQ=255)                                                                            cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACAt                                                                    >  7:65468/1‑148 (MQ=255)                                                                            cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGAAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACAt                                                                    >  2:72769/1‑148 (MQ=255)                                                                            cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGAAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACAt                                                                    >  2:78187/1‑148 (MQ=255)                                                                             ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCg                                                                                             <  3:338151/122‑1 (MQ=255)                                                                             ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCg                                                                                             >  4:338151/1‑122 (MQ=255)                                                                              cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCt                                                                  >  1:257783/1‑148 (MQ=255)                                                                              cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCt                                                                  >  6:319321/1‑148 (MQ=255)                                                                               aaTTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCtt                                                                 <  1:296094/148‑1 (MQ=255)                                                                                 ttttGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTc                                                               >  4:272726/1‑148 (MQ=255)                                                                                      aaaaTATCGAATGCACTAATCATATCCACACCCTCGGATTGCTGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTa                                                                                                  >  5:248415/1‑108 (MQ=255)                                                                                      aaaaTATCGAATGCACTAATCATATCCACACCCTCGGATTGCTGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTa                                                                                                  <  6:248415/108‑1 (MQ=255)                                                                                          tatCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAAcgc                                                      >  4:192060/1‑148 (MQ=255)                                                                                                     cTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGAAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGcac                                           <  1:72769/148‑1 (MQ=255)                                                                                                     cTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGAAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGcac                                           <  1:78187/148‑1 (MQ=255)                                                                                                       aaTCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGcaca                                          >  6:243898/1‑147 (MQ=255)                                                                                                        ataatatCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCATACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACAGCGATGGCTTCAATTTCCAGCTTCACAGCTTTCGGCAAACGCGCGACCAGcaca                                          >  1:393837/4‑146 (MQ=255)                                                                                                        aTCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGTACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGcacac                                         <  5:279432/147‑1 (MQ=255)                                                                                                        aTCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGcacaca                                        <  3:272726/148‑1 (MQ=255)                                                                                                          cATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGc                                      >  6:383724/1‑148 (MQ=255)                                                                                                           atatCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   <  1:256609/86‑1 (MQ=255)                                                                                                           atatCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGt                                                                                                   >  2:256609/1‑86 (MQ=255)                                                                                                              tCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCaa                                                          >  6:288195/1‑124 (MQ=255)                                                                                                              tCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCaa                                                          <  5:288195/124‑1 (MQ=255)                                                                                                               ccACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCgg                                 >  3:77557/1‑148 (MQ=255)                                                                                                                cacaCCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCggg                                >  4:175583/1‑148 (MQ=255)                                                                                                                 acacCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATgg                                                                                         >  3:78480/1‑90 (MQ=255)                                                                                                                 acacCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATgg                                                                                         <  4:78480/90‑1 (MQ=255)                                                                                                                 acacCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGt                               <  2:257783/148‑1 (MQ=255)                                                                                                                 acacCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTACCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGt                               >  2:383957/1‑148 (MQ=255)                                                                                                                      cTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCttt                                                                <  8:397139/110‑1 (MQ=255)                                                                                                                      cTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCttt                                                                >  7:397139/1‑110 (MQ=255)                                                                                                                           aTTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTc                     >  5:44758/1‑148 (MQ=255)                                                                                                                                   tCAGTGAAGTGGAGCGGAACGACCTTACGCCCGTCCCGCTCACGAGGCTTTAAGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTAGCctgctg             >  5:260507/1‑148 (MQ=255)                                                                                                                                   tCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTAGGATAGGTCGCCTGATg             >  6:274053/1‑148 (MQ=255)                                                                                                                                        gAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTAAGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCAt        >  1:11833/1‑148 (MQ=255)                                                                                                                                           gTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGTGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCga     <  1:300717/148‑1 (MQ=255)                                                                                                                                             ggAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAAcg                                                       >  7:201492/1‑96 (MQ=255)                                                                                                                                             ggAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAAcg                                                       <  8:201492/96‑1 (MQ=255)                                                                                                                                              gAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCgaaga  <  3:175583/148‑1 (MQ=255)                                                                                                                                              gAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCgaag   <  3:192060/147‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    GCTACTTTCCAGCCGATCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCGAAGA  >  NC_000913/3259542‑3259830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑