Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,584,549 A→G 5.6% V326A (GTG→GCG)  aegA ← putative oxidoreductase, FeS binding subunit/NAD/FAD‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,584,5490AG 5.6% 221.6 / 2.8 71V326A (GTG→GCG) aegAputative oxidoreductase, FeS binding subunit/NAD/FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (33/34);  new base G (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGAAAGAAGGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGCGGACAAA  >  NC_000913/2584404‑2584693
                                                                                                                                                 |                                                                                                                                                
tttGAAAGAAGGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACt                                                                                                                                                >  5:122053/1‑148 (MQ=255)
      agaagGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTg                                                                                                                                          <  2:386166/148‑1 (MQ=255)
      agaagGAATGCCGAAAGTGAGCAAGCCAACGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACATGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTg                                                                                                                                          <  1:378156/148‑1 (MQ=255)
        aagGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGa                                                                                                                                         >  3:176818/1‑147 (MQ=255)
        aagGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAc                                                                                                                                        <  6:122053/148‑1 (MQ=255)
                    aaGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAc                                                                                                                                        >  2:223826/1‑136 (MQ=255)
                    aaGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAc                                                                                                                                        <  1:223826/136‑1 (MQ=255)
                    aaGTGAGCAAGCCACCGATTTCTCGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTc                                                                                                                            <  4:176818/148‑1 (MQ=255)
                     aGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCa                                                                                                                           <  8:137612/148‑1 (MQ=255)
                              gCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAgg                                                                                                                         <  6:244219/141‑1 (MQ=255)
                              gCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAgg                                                                                                                         >  5:244219/1‑141 (MQ=255)
                               ccACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGTTGAc                                                                                                                                        >  7:43635/1‑125 (MQ=255)
                               ccACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGTTGAc                                                                                                                                        <  8:43635/125‑1 (MQ=255)
                                   cGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAc                                                                                                                                        <  6:67348/121‑1 (MQ=255)
                                   cGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAc                                                                                                                                        >  5:67348/1‑121 (MQ=255)
                                   cGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCttt                                                                                                             >  7:23620/1‑148 (MQ=255)
                                         cTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCa                                                                                                        <  8:23620/147‑1 (MQ=255)
                                                    tCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCCCAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGAc                                                                                                                                        >  2:230853/1‑104 (MQ=255)
                                                    tCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGAc                                                                                                                                        <  1:225009/104‑1 (MQ=255)
                                                    tCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGAc                                                                                                                                        >  2:225009/1‑104 (MQ=255)
                                                    tCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGAc                                                                                                                                        <  1:230853/104‑1 (MQ=255)
                                                        acacCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCaa                                                                                                                  >  6:316695/1‑122 (MQ=255)
                                                        acacCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCaa                                                                                                                  <  5:316695/122‑1 (MQ=255)
                                                                  ccccACGCCATTGCGGGTCAGAACATCCGCACAGTACAGCCCTGCCGGACCTCCACCGATAATCGCCACCCGATTGTCCACTTTGGTGACATGGCTTAAGTCACGACCCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGcttac                                                                               >  4:26716/1‑143 (MQ=255)
                                                                  ccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCa                                                                              <  2:37454/148‑1 (MQ=255)
                                                                      aCGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGa                                                                                                                                         >  2:339541/1‑85 (MQ=255)
                                                                      aCGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGa                                                                                                                                         <  1:339541/85‑1 (MQ=255)
                                                                        gCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTg                                                                        >  3:256294/1‑148 (MQ=255)
                                                                          cATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGcc                                                                      >  8:243571/1‑148 (MQ=255)
                                                                             tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCg                                                                     >  7:397676/1‑146 (MQ=255)
                                                                             tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCg                                                                     <  8:397676/146‑1 (MQ=255)
                                                                             tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGa                                                                    >  8:154733/1‑147 (MQ=255)
                                                                             tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACACGCTTGTCCACTTTGGTGACAAGGCATAATTCAGGACGCCAACCTTTCTCCAACGCCTGATCTGAAATGTAGCGTTCAATGATGACGa                                                                    >  6:194427/1‑147 (MQ=255)
                                                                               cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAg                                                                 <  4:256294/148‑1 (MQ=255)
                                                                                   tCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTAc                                                             >  2:354235/1‑148 (MQ=255)
                                                                                        aCATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaa                                                                                          <  8:388811/114‑1 (MQ=255)
                                                                                        aCATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaa                                                                                          >  7:388811/1‑114 (MQ=255)
                                                                                             cGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGc                                                   >  7:105377/1‑148 (MQ=255)
                                                                                               cacaGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTc                                                 <  5:194427/148‑1 (MQ=255)
                                                                                                  aGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTg                                                    >  4:305274/1‑142 (MQ=255)
                                                                                                  aGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTg                                                    <  3:305274/142‑1 (MQ=255)
                                                                                                   ggCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATcg                                             >  4:147588/1‑148 (MQ=255)
                                                                                                    gCCAGCCCTGCCGGACCTGCACCGATAATCGCCGCCCGCTTGTCCACGTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATcgc                                            >  8:292484/1‑148 (MQ=255)
                                                                                                    gCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCGCATcgc                                            <  3:74597/148‑1 (MQ=255)
                                                                                                       aGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGaa                                         <  7:154733/148‑1 (MQ=255)
                                                                                                        gCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAAt                                        <  3:147588/148‑1 (MQ=255)
                                                                                                           cTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGt                                     <  8:105377/148‑1 (MQ=255)
                                                                                                           cTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGt                                     <  7:243571/148‑1 (MQ=255)
                                                                                                            tGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCa                                                                              >  4:290790/1‑106 (MQ=255)
                                                                                                            tGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCa                                                                              <  3:290790/106‑1 (MQ=255)
                                                                                                            tGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATa                                                                  >  1:8588/1‑118 (MQ=255)
                                                                                                             gCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATc                                              <  1:328552/137‑1 (MQ=255)
                                                                                                             gCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATc                                              >  2:328552/1‑137 (MQ=255)
                                                                                                             gCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTa                                    >  5:81358/1‑147 (MQ=255)
                                                                                                              ccGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTa                                    <  1:354235/146‑1 (MQ=255)
                                                                                                              ccGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTAc                                   <  6:81358/147‑1 (MQ=255)
                                                                                                              acGGACCTGAACAGATAATCGCCACCCGCTTTTCCACTTTGGTGACATGTCTTAAGTCAGTACGCCAACCTTTCGCCAACGCCTTATCTGAAATTTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGATCATCGCGAATAGTAc                                   <  3:26716/146‑1 (MQ=255)
                                                                                                                   ccTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCAc                             >  5:329036/1‑148 (MQ=255)
                                                                                                                    cTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCAcc                            <  5:212348/148‑1 (MQ=255)
                                                                                                                      gCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAAt                                        >  3:176603/1‑134 (MQ=255)
                                                                                                                      gCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAAt                                        <  4:176603/134‑1 (MQ=255)
                                                                                                                      gCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTAc                                   >  7:206254/1‑139 (MQ=255)
                                                                                                                      gCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTAc                                   <  8:206254/139‑1 (MQ=255)
                                                                                                                        aCCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTa                                    <  4:12910/136‑1 (MQ=255)
                                                                                                                        aCCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTa                                    >  3:12910/1‑136 (MQ=255)
                                                                                                                              aaTCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACaaa                  >  2:249339/1‑148 (MQ=255)
                                                                                                                                       ccGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGc         >  3:332257/1‑148 (MQ=255)
                                                                                                                                        cGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGCg        >  6:202136/1‑148 (MQ=255)
                                                                                                                                             gTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGCGGACa    >  2:167993/1‑147 (MQ=255)
                                                                                                                                             gTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGCGGACa    <  4:146292/147‑1 (MQ=255)
                                                                                                                                              tCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGCGGACaaa  <  6:329036/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTGAAAGAAGGAATGCCGAAAGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAGCGTTCAATGTTGCCGATAGTTACCGCGCCGTGCTCATCGCGAATAGTACAGGCACCTTCACACAAACGGTCTTGCGGACAAA  >  NC_000913/2584404‑2584693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: