Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,172,148 G→A 7.4% A606V (GCG→GTG)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,172,1480GA 7.4% 257.3 / 7.4 82A606V (GCG→GTG) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (41/34);  new base A (3/3);  total (44/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

CTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACTTTTTCC  >  NC_000913/1172015‑1172291
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cTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGAt                                                                                                                                           <  8:202067/140‑1 (MQ=255)
cTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGAt                                                                                                                                           >  7:202067/1‑140 (MQ=255)
  tgtgGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGt                                                                                                                                 >  8:114344/1‑148 (MQ=255)
   gtgGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGtt                                                                                                                                >  6:352516/1‑148 (MQ=255)
       ttATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCaa                                                                                                                            <  7:114344/148‑1 (MQ=255)
          tCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACg                                                                                                                         >  2:83647/1‑148 (MQ=255)
             aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGTACCTGAt                                                                                                                                           >  8:50605/1‑127 (MQ=255)
             aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGTACCTGAt                                                                                                                                           >  8:44353/1‑127 (MQ=255)
             aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGTACCTGAt                                                                                                                                           <  7:50605/127‑1 (MQ=255)
             aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGTACCTGAt                                                                                                                                           <  7:44353/127‑1 (MQ=255)
             aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCACCTGAt                                                                                                                                           >  6:385563/1‑127 (MQ=255)
             aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCACCTGAt                                                                                                                                           <  5:385563/127‑1 (MQ=255)
              cTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGaa                                                                                                                     <  5:352516/148‑1 (MQ=255)
               agCCAGGAAAGCTGCGCGCATCGCCACTTCTGTATTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACAAGACGCTAAGTACCGCATAAATGGCCTGCGCCTGATCCGGCGTGGATTCAAACggt                                                                                                                       >  1:304369/2‑144 (MQ=255)
                gCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGt                                                                                                                                 >  2:288244/1‑134 (MQ=255)
                gCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGt                                                                                                                                 <  1:288244/134‑1 (MQ=255)
                gCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGc                                                                                                                   >  5:227727/1‑148 (MQ=255)
                gCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGc                                                                                                                   >  7:315199/1‑148 (MQ=255)
                 ccAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGc                                                                                                                   <  1:83647/147‑1 (MQ=255)
                    ggAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTc                                                                                                               >  6:284117/1‑148 (MQ=255)
                      aaaGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCg                                                                                                              >  1:193469/1‑147 (MQ=255)
                      aaaGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCg                                                                                                              >  3:218200/1‑147 (MQ=255)
                         gCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAg                                                                                                           >  7:303841/1‑147 (MQ=255)
                                  aTCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  6:227727/148‑1 (MQ=255)
                                  aTCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  4:218200/148‑1 (MQ=255)
                                  aTCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  6:71122/148‑1 (MQ=255)
                                    cGCCACTTGTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   <  5:28580/144‑1 (MQ=255)
                                    cGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   >  6:96098/1‑144 (MQ=255)
                                    cGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   <  5:96098/144‑1 (MQ=255)
                                    cGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   >  6:28580/1‑144 (MQ=255)
                                     gCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCg                                                                                                              >  7:282218/1‑132 (MQ=255)
                                     gCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCg                                                                                                              <  8:282218/132‑1 (MQ=255)
                                      ccACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGGTTCAAACGGGAAGCTGTCGCAGCACACCTGATACTg                                                                                             >  5:123834/1‑148 (MQ=255)
                                          ttCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 >  4:200339/1‑140 (MQ=255)
                                          ttCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  3:200339/140‑1 (MQ=255)
                                          ttCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 >  7:35249/1‑140 (MQ=255)
                                          ttCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  8:35249/140‑1 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   <  2:203253/137‑1 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   >  1:203253/1‑137 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  7:179/139‑1 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 >  3:268088/1‑139 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  4:268088/139‑1 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 >  8:179/1‑139 (MQ=255)
                                           tCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCAc                                                                                        >  2:316446/1‑148 (MQ=255)
                                            cTGTTTTACCAAAGCCAACCTCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCTCATGAATTGCCTGCGCCTGATCCGGCGTGGGTTCAACCGGGAAGCTGTCGCAGCACCCATGCTACTGCTCAc                                                                                        >  2:81299/1‑147 (MQ=255)
                                               ttttACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATc                                                                                    >  6:195123/1‑148 (MQ=255)
                                                     cAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGttt                                                                              <  5:284117/148‑1 (MQ=255)
                                                            aaCATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAAcgcg                                                                       <  1:316446/148‑1 (MQ=255)
                                                            aaCATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAAcgcg                                                                       <  8:315199/148‑1 (MQ=255)
                                                              cATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  4:171188/120‑1 (MQ=255)
                                                              cATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 >  3:171188/1‑120 (MQ=255)
                                                                    cgcACACCAGACGATCCCTTGCCCTCGGGTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCAGGATCCGGCGGGGTTTCAAACGGGAAGCTGGCGCAGACCCACTGATCCTGCTCACGATCGTGTTTAAACGCGAAGCCctc                                                               <  1:81299/148‑1 (MQ=255)
                                                                       acacCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAAcgcg                                                                       <  6:249124/137‑1 (MQ=255)
                                                                       acacCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAAcgcg                                                                       >  5:249124/1‑137 (MQ=255)
                                                                          ccAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGc                                                                                                             >  4:85367/1‑96 (MQ=255)
                                                                          ccAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGc                                                                                                             <  3:85367/96‑1 (MQ=255)
                                                                           cAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTggcg                                                        <  8:303841/148‑1 (MQ=255)
                                                                           cAGACGATCCATTGCCAGCGGCTGACACAAGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCACACGATCCTGTTTACACGCGAAGCCCTCTTTggcg                                                        >  1:370315/1‑148 (MQ=255)
                                                                              aCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   >  8:317789/1‑102 (MQ=255)
                                                                              aCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTg                                                                                                   <  7:317789/102‑1 (MQ=255)
                                                                                  tCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGaacaac                                                                                                     >  7:247963/1‑96 (MQ=255)
                                                                                  tCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGaacaac                                                                                                     <  8:247963/96‑1 (MQ=255)
                                                                                    cATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTg                                                <  5:195123/147‑1 (MQ=255)
                                                                                       tGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGaaca                                                                                                       >  5:260383/1‑89 (MQ=255)
                                                                                       tGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGaaca                                                                                                       <  6:260383/89‑1 (MQ=255)
                                                                                       tGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGcgcg                                            >  4:137917/1‑148 (MQ=255)
                                                                                          cAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGcgcg                                            >  2:52538/1‑145 (MQ=255)
                                                                                          cAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAg                                         <  1:52538/148‑1 (MQ=255)
                                                                                           aGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGa                                        <  2:193469/148‑1 (MQ=255)
                                                                                                   acacATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAg                                 >  1:310330/1‑147 (MQ=255)
                                                                                                    cacaTGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGc                                >  6:92017/1‑147 (MQ=255)
                                                                                                       aTGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAAtt                            >  5:122878/1‑148 (MQ=255)
                                                                                                         gTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTcc                          >  1:236154/1‑148 (MQ=255)
                                                                                                                aGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCgccgcc                    <  3:137917/147‑1 (MQ=255)
                                                                                                                    ccGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACAt                <  5:92017/147‑1 (MQ=255)
                                                                                                                          tAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 >  8:194951/1‑60 (MQ=255)
                                                                                                                          tAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGAt                                                                                                 <  7:194951/60‑1 (MQ=255)
                                                                                                                          tAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCAc         >  2:154625/1‑148 (MQ=255)
                                                                                                                          tAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCAc         >  1:97866/1‑148 (MQ=255)
                                                                                                                          tAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCAc         >  8:336227/1‑148 (MQ=255)
                                                                                                                            ctGGCCTGCTCCTGAGCCGGCGTGGTTTCAACCGGGAAGCTGTCCCAGAAGAACTGATACTGCTCTCGATCGTGGTAAACCGCGAAGGCCGCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATGCCGCCGCCACATCCCCCACt        <  2:304369/146‑1 (MQ=255)
                                                                                                                                 cTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACTTTTTcc  <  2:236154/148‑1 (MQ=255)
                                                                                                                                     gcCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACtt       >  8:197116/1‑139 (MQ=255)
                                                                                                                                     gcCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACtt       <  7:197116/139‑1 (MQ=255)
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CTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACTTTTTCC  >  NC_000913/1172015‑1172291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: