Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,126,716	A→T	5.4%	D187V (GAT→GTT)	yceH →	UPF0502 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,126,716	0	A	T	5.4%	244.4 / 3.6	74	D187V (GAT→GTT)	yceH	UPF0502 family protein
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base T (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

AACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGT  >  NC_000913/1126572‑1126860                                                                                                                                                 |                                                                                                                                                 aacTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAttt                                                                                                                                               <  4:160358/148‑1 (MQ=255) aacTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAttt                                                                                                                                               <  2:304068/148‑1 (MQ=255)  acTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAttt                                                                                                                                               <  3:100108/147‑1 (MQ=255)  acTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAttt                                                                                                                                               >  4:100108/1‑147 (MQ=255)   cTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTAc                                                                                                                                             <  4:174553/148‑1 (MQ=255)     ggaaaaTCTCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAg                                                                                                                                           >  3:44895/4‑148 (MQ=255)     ggCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAg                                                                                                                                           >  4:81928/1‑148 (MQ=255)          aTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCTGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTa                                                                                                                                              <  4:344928/140‑1 (MQ=255)          aTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTa                                                                                                                                              >  5:237776/1‑140 (MQ=255)          aTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTa                                                                                                                                              <  6:237776/140‑1 (MQ=255)          aTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCCGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTa                                                                                                                                              >  3:344928/1‑140 (MQ=255)             gcgAAGATGGTCCTTTTGTTGTGCGTGGGGCCCGCGAACCGTGTAAACCGGAAAAGCTCTACATGCATCTTTTGTGTGTTGAGGTTGATGATCAGCATGCCGTGACTGATATGGCGAACGCGGTTGACGGTGATTTACAGGCCCGCGt                                                                                                                                   <  6:106041/148‑1 (MQ=255)                       tCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTg                                                                                                                         >  1:227370/1‑148 (MQ=255)                            ttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCATGGAAAt                                                                                                                    >  3:64012/1‑148 (MQ=255)                               tggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGa                                                                                                                 <  2:227370/148‑1 (MQ=255)                                ggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGCTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGaa                                                                                                                <  5:268484/148‑1 (MQ=255)                                 gtgCGGCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGaa                                                                                                                <  4:44895/147‑1 (MQ=255)                                                 aaCCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGaaa                                                                                                 >  6:203792/1‑146 (MQ=255)                                                     gggTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTgg                                                                                                                        >  6:293611/1‑119 (MQ=255)                                                     gggTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTgg                                                                                                                        <  5:293611/119‑1 (MQ=255)                                                       gTAAACGCGAAAACCGCTACATGAATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGACCGCGTCGAAGCCCTGGACATCGAAGTGGCAGAACTGAAACAGCGTCt                                                                                         >  8:215211/1‑148 (MQ=255)                                                        tAAACTCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGTCGGATATGTCGAACGCGGTTGACGGTGATTTAGAGGCCCGCGTCGAAGCCCTGGAAATCGAAGT‑GCAGAACTGAAACAGCGTCTTg                                                                                       <  1:111914/148‑1 (MQ=255)                                                                        tACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGccc                                                                                                                           >  2:320576/1‑97 (MQ=255)                                                                        tACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGccc                                                                                                                           <  7:340861/97‑1 (MQ=255)                                                                        tACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGccc                                                                                                                           >  8:340861/1‑97 (MQ=255)                                                                        tACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGccc                                                                                                                           <  1:320576/97‑1 (MQ=255)                                                                            tGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCt                                                                    >  6:62784/1‑148 (MQ=255)                                                                            tGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCt                                                                    >  4:70455/1‑148 (MQ=255)                                                                             gCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTg                                                                   >  1:192282/1‑148 (MQ=255)                                                                              cATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTg                                                                   <  3:70455/147‑1 (MQ=255)                                                                                 cTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGc                                                                                                                             >  3:195570/1‑86 (MQ=255)                                                                                 cTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGc                                                                                                                             <  4:195570/86‑1 (MQ=255)                                                                                 cTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGACCACGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCACAtaggg                                                                  >  4:98563/1‑141 (MQ=255)                                                                                   tttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTg                                                                               <  7:37469/130‑1 (MQ=255)                                                                                   tttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTg                                                                               >  8:37469/1‑130 (MQ=255)                                                                                        gtggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCAt                                                                      >  1:135670/1‑134 (MQ=255)                                                                                        gtggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCAt                                                                      <  2:135670/134‑1 (MQ=255)                                                                                        gtggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAg                                                        >  3:184046/1‑148 (MQ=255)                                                                                          ggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGtt                                                                                <  8:274728/122‑1 (MQ=255)                                                                                          ggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGtt                                                                                >  7:274728/1‑122 (MQ=255)                                                                                          ggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGACCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGt                                                       >  2:776/1‑147 (MQ=255)                                                                                             gAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaa                                                    >  2:349671/1‑147 (MQ=255)                                                                                              aGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCa                                                                       >  8:208918/1‑127 (MQ=255)                                                                                              aGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCa                                                                       <  7:208918/127‑1 (MQ=255)                                                                                               ggTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaaa                                                 <  1:349671/148‑1 (MQ=255)                                                                                                 ttGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGTAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAt                                               >  3:235059/1‑148 (MQ=255)                                                                                                   gAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAtt                                              <  1:776/147‑1 (MQ=255)                                                                                                    aaGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTa                                             <  3:81928/147‑1 (MQ=255)                                                                                                    aaGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTGGATTAGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAACATTAc                                            >  6:75057/1‑148 (MQ=255)                                                                                                      gATCATCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCTTCGAAGCCCTGGTAATCGAAGTGGCAGAAATGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGt                                          <  3:98563/148‑1 (MQ=255)                                                                                                      gATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGTTTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACg                                           >  6:356013/1‑147 (MQ=255)                                                                                                      gATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGTTTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACg                                           >  6:350492/1‑147 (MQ=255)                                                                                                       aTCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGTTTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTa                                         <  5:350492/148‑1 (MQ=255)                                                                                                       aTCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGTTTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTa                                         <  5:356013/148‑1 (MQ=255)                                                                                                        tCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAtt                                              >  5:254265/1‑142 (MQ=255)                                                                                                        tCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAtt                                              <  6:254265/142‑1 (MQ=255)                                                                                                        tCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTa                                         >  2:338819/1‑147 (MQ=255)                                                                                                          aGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCg                                      >  2:344027/1‑148 (MQ=255)                                                                                                             cggcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCg                                   >  8:79913/1‑148 (MQ=255)                                                                                                               gcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTa                                 <  2:192282/148‑1 (MQ=255)                                                                                                               gcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTa                                 <  1:338819/148‑1 (MQ=255)                                                                                                                   tGACGGATATGTCTAACGCGGTTGACGGTGATTTACAGGACCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTTAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGATATTAAAGTGAAAAAATTACGTATCGGCGTAGTgg                             <  5:75057/148‑1 (MQ=255)                                                                                                                   tGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGt                               >  2:245686/1‑146 (MQ=255)                                                                                                                   tGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGt                               <  1:245686/146‑1 (MQ=255)                                                                                                                    gACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAg                                >  3:42883/1‑144 (MQ=255)                                                                                                                    gACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAg                                <  4:42883/144‑1 (MQ=255)                                                                                                                    gACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTgg                             >  5:342432/1‑147 (MQ=255)                                                                                                                       ggATATGTCGAACTCGGTTGACGGTTATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGATGTGGCAGAACTGTAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGAtt                         <  7:215211/148‑1 (MQ=255)                                                                                                                       ggATATGTCGAACGCGCTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGAtt                         <  4:64012/148‑1 (MQ=255)                                                                                                                         atatGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAg                       <  5:62784/148‑1 (MQ=255)                                                                                                                         atatGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAg                       <  5:203792/148‑1 (MQ=255)                                                                                                                                        ttGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTa                                 >  3:288744/1‑123 (MQ=255)                                                                                                                                        ttGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTa                                 <  4:288744/123‑1 (MQ=255)                                                                                                                                              gTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGt  <  4:184046/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 AACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGT  >  NC_000913/1126572‑1126860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑