Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 926,398 T→C 5.1% T16A (ACG→GCG)  infA ← translation initiation factor IF‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913926,3980TC 5.1% 244.7 / 3.6 78T16A (ACG→GCG) infAtranslation initiation factor IF‑1
Reads supporting (aligned to +/- strand):  ref base T (37/37);  new base C (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

TTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCGCAACAAACAGG  >  NC_000913/926253‑926543
                                                                                                                                                 |                                                                                                                                                 
ttGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACAGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGTTATTAGGCAACGatt                                                                                                                                                 >  5:209103/1‑148 (MQ=255)
       ggTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAAc                                                                                                                                          >  1:156202/1‑148 (MQ=255)
          cGTACGGGGTCAGTTCTACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTAGCGGTGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAAGATGGTATTAGGCAACGTTTCAAGAACGGt                                                                                                                                       <  3:313619/148‑1 (MQ=255)
          cGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACgg                                                                                                                                        >  4:303932/1‑147 (MQ=255)
             aCGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTAcc                                                                                                                                    >  5:149018/1‑148 (MQ=255)
              cGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCt                                                                                                                                   >  6:112919/1‑148 (MQ=255)
              cGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCt                                                                                                                                   >  5:32127/1‑148 (MQ=255)
                 ggTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGc                                                                                                                                <  3:303932/148‑1 (MQ=255)
                    cAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCAtt                                                                                                                             >  8:362799/1‑148 (MQ=255)
                     atttCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCTGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCAtt                                                                                                                             <  7:344961/145‑1 (MQ=255)
                      gTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTc                                                                                                                           <  2:174235/148‑1 (MQ=255)
                        tCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTc                                                                                                                                                >  5:150566/1‑125 (MQ=255)
                        tCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTc                                                                                                                                                <  6:150566/125‑1 (MQ=255)
                        tCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTAc                                                                                                                                     <  4:75424/136‑1 (MQ=255)
                        tCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTAc                                                                                                                                     >  3:75424/1‑136 (MQ=255)
                        tCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCaa                                                                                                                         >  5:118896/1‑148 (MQ=255)
                        tCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCaa                                                                                                                         >  6:226074/1‑148 (MQ=255)
                             aGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTg                                                                                                                    <  5:226074/148‑1 (MQ=255)
                                    ttGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGc                                                                                                                                <  7:391503/129‑1 (MQ=255)
                                    ttGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGc                                                                                                                                >  8:391503/1‑129 (MQ=255)
                                     tGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGtcttct                                                                                                              <  6:32127/146‑1 (MQ=255)
                                       tagcacGTGAGGATGATGATGTTGTTTTTGCGCATTTTACCGGAGATGTTTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAAGATGGTATTAGGCAACGTTTCTTGAACGGTAACTTGCATTTCAATATTGTCTTCTTTgg                                                                                                          <  6:209103/143‑1 (MQ=255)
                                           ccGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCAt                                                                                                      >  8:358751/1‑148 (MQ=255)
                                            cGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGtcttc                                                                                                               <  1:2698/138‑1 (MQ=255)
                                            cGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGtcttc                                                                                                               >  2:2698/1‑138 (MQ=255)
                                               cAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTa                                                                                                   >  8:53640/1‑147 (MQ=255)
                                                aGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAAt                                                                                                 <  7:362799/148‑1 (MQ=255)
                                                      cGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGc                                                                                                                                <  2:326252/111‑1 (MQ=255)
                                                      cGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGc                                                                                                                                >  1:326252/1‑111 (MQ=255)
                                                            tAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTAt                                                                                     >  8:316347/1‑148 (MQ=255)
                                                              gTTTTTGCTCATTTTACCGGAGATGTTGGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCCACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  2:328103/147‑1 (MQ=255)
                                                              gTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  3:247219/147‑1 (MQ=255)
                                                              gTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  7:53640/147‑1 (MQ=255)
                                                              gTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  5:112919/147‑1 (MQ=255)
                                                              gTTTTTGCGCATTTTACCGGAGATGTCTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  6:118896/147‑1 (MQ=255)
                                                               tttttGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCAc                                                                                  >  6:49603/1‑148 (MQ=255)
                                                                 tttGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACt                                                                                 <  7:358751/147‑1 (MQ=255)
                                                                   tGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTAc                                                                               <  5:49603/147‑1 (MQ=255)
                                                                          tttACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGtct                                                                                                                 <  4:318026/106‑1 (MQ=255)
                                                                          tttACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGtct                                                                                                                 >  3:318026/1‑106 (MQ=255)
                                                                             aCCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGa                                                                     <  7:316347/147‑1 (MQ=255)
                                                                                   gaTGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCa                                                              <  4:151289/148‑1 (MQ=255)
                                                                                       tgtgCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGAt                                                          <  2:156202/148‑1 (MQ=255)
                                                                                       tgtgCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATATCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGAt                                                          <  6:149018/148‑1 (MQ=255)
                                                                                        gtgCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCgg                                                                >  8:308753/1‑141 (MQ=255)
                                                                                        gtgCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCgg                                                                <  7:308753/141‑1 (MQ=255)
                                                                                          gCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTcttctt                                                                                                             >  2:20747/1‑94 (MQ=255)
                                                                                          gCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTcttctt                                                                                                             <  1:20747/94‑1 (MQ=255)
                                                                                               aaCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    >  5:265962/1‑114 (MQ=255)
                                                                                               aaCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  6:265962/114‑1 (MQ=255)
                                                                                               aaCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGc                                                     >  1:195575/1‑145 (MQ=255)
                                                                                               aaCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGc                                                     <  2:195575/145‑1 (MQ=255)
                                                                                               aaCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCg                                                   >  7:227146/1‑147 (MQ=255)
                                                                                               aaCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCg                                                   >  5:348034/1‑147 (MQ=255)
                                                                                                aCCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGa                                                  <  8:227146/147‑1 (MQ=255)
                                                                                                 ccACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATa                                                         <  1:318154/139‑1 (MQ=255)
                                                                                                 ccACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATa                                                         >  2:318154/1‑139 (MQ=255)
                                                                                                     gTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCt                                            >  4:143917/1‑148 (MQ=255)
                                                                                                      tGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGa                                                  >  7:393603/1‑141 (MQ=255)
                                                                                                      tGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGa                                                  <  8:393603/141‑1 (MQ=255)
                                                                                                       gACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTg                                           >  3:268613/1‑147 (MQ=255)
                                                                                                           gTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    >  8:260916/1‑102 (MQ=255)
                                                                                                           gTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATc                                                                                    <  7:260916/102‑1 (MQ=255)
                                                                                                            ttttCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAAt                                      >  1:46973/1‑147 (MQ=255)
                                                                                                             tttCTAACTCTACGCGGAACATGGTATTAGGCAACGCTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGaa                                                 >  7:31572/1‑135 (MQ=255)
                                                                                                             tttCTAACTCTACGCGGAACATGGTATTAGGCAACGCTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGaa                                                 >  7:37658/1‑135 (MQ=255)
                                                                                                             tttCTAACTCTACGCGGAACATGGTATTAGGCAACGCTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGaa                                                 <  8:37658/135‑1 (MQ=255)
                                                                                                             tttCTAACTCTACGCGGAACATGGTATTAGGCAACGCTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGaa                                                 <  8:31572/135‑1 (MQ=255)
                                                                                                                  aaCTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTaa                                >  7:147634/1‑147 (MQ=255)
                                                                                                                   aCTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTaa                                <  6:348034/146‑1 (MQ=255)
                                                                                                                                   ggTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATc              >  3:217823/1‑148 (MQ=255)
                                                                                                                                   ggTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATc              >  2:78866/1‑148 (MQ=255)
                                                                                                                                    gTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCg             <  3:143917/148‑1 (MQ=255)
                                                                                                                                       ttAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCGcaa          >  7:13898/1‑148 (MQ=255)
                                                                                                                                       ttAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCGca           <  4:268613/147‑1 (MQ=255)
                                                                                                                                          ggCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCGcaaca        >  6:179285/1‑147 (MQ=255)
                                                                                                                                             aaCGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATa                                                         >  7:162244/1‑95 (MQ=255)
                                                                                                                                             aaCGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATa                                                         <  8:162244/95‑1 (MQ=255)
                                                                                                                                               cGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCGCAACAAACAgg  >  4:60406/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGCGCATTTTACCGGAGATGTGTGCAGTAACCACGTGACCGTTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTGCGCGCTAAATCGCAACAAACAGG  >  NC_000913/926253‑926543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: