Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	829,686	G→A	10.2%	A96V (GCG→GTG)	ybhG ←	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,686	0	G	A	10.2%	116.6 / 2.8	39	A96V (GCG→GTG)	ybhG	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor
Reads supporting (aligned to +/- strand):  ref base G (17/18);  new base A (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATAGTGCGGCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGA  >  NC_000913/829543‑829827                                                                                                                                                |                                                                                                                                              aaaTAGTGCGGCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGcc                                                                                                                                             >  8:366973/1‑146 (MQ=255) aaaTAGTGCGGCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGcc                                                                                                                                             <  7:366973/146‑1 (MQ=255)       tGCGGCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCAc                                                                                                                                     >  8:47559/1‑148 (MQ=255)           gCTTTTCCACAACCCTTGCTGGCTGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCTATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGaa                                                                                                                                 <  5:128515/148‑1 (MQ=255)           gCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGaa                                                                                                                                 <  1:158263/148‑1 (MQ=255)           gCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGaa                                                                                                                                 <  6:334020/148‑1 (MQ=255)                     aaCCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCtt                                                                                                                       <  5:268979/148‑1 (MQ=255)                                    ttATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGa                                                                                                                                  <  3:341315/122‑1 (MQ=255)                                    ttATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGa                                                                                                                                  >  4:341315/1‑122 (MQ=255)                                       taGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCa                                                                                                      <  5:69202/147‑1 (MQ=255)                                              tCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCACTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGa                                                                                                                                  >  2:228665/1‑112 (MQ=255)                                              tCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCACTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGa                                                                                                                                  <  1:228665/112‑1 (MQ=255)                                                       aGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTtgt                                                                                      >  2:355579/1‑147 (MQ=255)                                                           aTAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATcc                                                                                 <  8:37492/148‑1 (MQ=255)                                                             aGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAAtctc                                                                                                 >  2:219129/1‑130 (MQ=255)                                                             aGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAAtctc                                                                                                 <  1:219129/130‑1 (MQ=255)                                                                              ttCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCACCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGc                                                              >  5:99389/1‑148 (MQ=255)                                                                              ttCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCACCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGc                                                              >  7:261761/1‑148 (MQ=255)                                                                               tCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCCGCACCTGGcc                                                             >  2:386238/1‑148 (MQ=255)                                                                                   cGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCt                                                         >  7:135796/1‑148 (MQ=255)                                                                                           cggcCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCACCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGc                                                 <  8:261761/148‑1 (MQ=255)                                                                                           cggcCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCACCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGc                                                 <  6:99389/148‑1 (MQ=255)                                                                                            ggcCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCa                                                <  1:355579/148‑1 (MQ=255)                                                                                                 gAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGcc                                                                         <  4:271966/118‑1 (MQ=255)                                                                                                 gAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGcc                                                                         >  3:271966/1‑118 (MQ=255)                                                                                                 gAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACACGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATctc                                            >  5:348857/1‑145 (MQ=255)                                                                                                  agggATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTTGCCCGCTTTGATAGCATCa                                             <  6:348857/142‑1 (MQ=255)                                                                                                       tttCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTc                                     <  7:47559/148‑1 (MQ=255)                                                                                                         tcttcATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCa                                   >  6:335638/1‑148 (MQ=255)                                                                                                                               gCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGAtt                       >  1:106313/1‑138 (MQ=255)                                                                                                                               gCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGAtt                       <  2:106313/138‑1 (MQ=255)                                                                                                                               gCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCa                     >  3:45265/1‑140 (MQ=255)                                                                                                                               gCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCa                     <  4:45265/140‑1 (MQ=255)                                                                                                                                       tCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTtgtg                                                                                     >  7:92961/1‑68 (MQ=255)                                                                                                                                       tCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTtgtg                                                                                     <  8:92961/68‑1 (MQ=255)                                                                                                                                       tCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAacac     >  4:79785/1‑148 (MQ=255)                                                                                                                                         aTACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACgg   <  8:135796/148‑1 (MQ=255)                                                                                                                                           aCTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGa  >  7:298267/1‑147 (MQ=255)                                                                                                                                           aCTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGa  >  3:284993/1‑147 (MQ=255)                                                                                                                                              gcgcCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCAc                                                                   <  1:256293/79‑1 (MQ=255)                                                                                                                                              gcgcCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCAc                                                                   >  2:256293/1‑79 (MQ=255)                                                                                                                                                |                                                                                                                                              AAATAGTGCGGCTTTTCCACAACCCTTGCTGGCGGTTATAGAAGTTCTGCGCATAGTCATAGGCGGCTTGCGCCTGTTTCACCGCTGCGGCGGCCTGAGCGATTTCTTCATTGCGATACCCGGCAAGCATCAGGTCATACTGCGCCTGTGCCACCGAAACACCCGCTTTCGCCTGCATCAGGGCAATCTCATACGGCTTGTGATCCAGTTCGCCCAGCACCTGGCCCGCTTTGATAGCATCACCTTCGTCCACCGCCAGCGATTCAACGCGCCCCCCAACACGGA  >  NC_000913/829543‑829827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑