Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 596,222 G→T 7.1% S208I (AGC→ATC)  cusC → copper/silver efflux system, outer membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913596,2220GT 7.1% 193.5 / 2.9 56S208I (AGC→ATC) cusCcopper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base G (25/27);  new base T (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TATTCTGCTGGTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAA  >  NC_000913/596085‑596361
                                                                                                                                         |                                                                                                                                           
tatTCTGCTGGTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGtt                                                                                                                                   <  4:3429/148‑1 (MQ=255)
 atTCTGCTGGTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTc                                                                                                                                  >  7:190161/1‑148 (MQ=255)
 atTCTGCTGGTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTc                                                                                                                                  >  8:370244/1‑148 (MQ=255)
 atTCTGCTGGTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTc                                                                                                                                  >  7:40628/1‑148 (MQ=255)
         ggTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTg                                                                                                                          >  3:41367/1‑148 (MQ=255)
              cTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAAc                                                                                                                      <  8:40628/147‑1 (MQ=255)
              cTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAAc                                                                                                                      <  7:370244/147‑1 (MQ=255)
                   gTCGCGCAAAGCTATTTCAATCAGCAACTGGGGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGc                                                                                                                                            <  3:99321/120‑1 (MQ=255)
                   gTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGc                                                                                                                                            >  4:99321/1‑120 (MQ=255)
                      gcgcAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTcgc                                                                                                              <  4:41367/147‑1 (MQ=255)
                           aaGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTg                                                                                                        <  4:75492/148‑1 (MQ=255)
                                aTTTCAATCAGCAACTGGCGTATGCGCAATTGCATATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGt                                                                                                                                    >  3:15312/1‑115 (MQ=255)
                                aTTTCAATCAGCAACTGGCGTATGCGCAATTGCATATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGt                                                                                                                                    <  4:15312/115‑1 (MQ=255)
                                 tttCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  7:70239/148‑1 (MQ=255)
                                 tttCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGa                                                                                                   >  6:152680/1‑147 (MQ=255)
                                  ttCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGa                                                                                                                        >  7:3190/1‑125 (MQ=255)
                                  ttCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGa                                                                                                                        <  8:3190/125‑1 (MQ=255)
                                  ttCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  8:190161/147‑1 (MQ=255)
                                   tCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGAGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAg                                                                                                >  3:207102/1‑148 (MQ=255)
                                      aTCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAAc                                                                                                                      <  2:336561/123‑1 (MQ=255)
                                      aTCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAAc                                                                                                                      >  1:336561/1‑123 (MQ=255)
                                      aTCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  >  5:15796/1‑143 (MQ=255)
                                      aTCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  6:15796/143‑1 (MQ=255)
                                        cAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTATCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  4:25615/141‑1 (MQ=255)
                                        cAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTATCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  2:238789/141‑1 (MQ=255)
                                        cAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTATCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  >  3:25615/1‑141 (MQ=255)
                                        cAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTATCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  >  1:238789/1‑141 (MQ=255)
                                        cAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  7:290144/141‑1 (MQ=255)
                                        cAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  >  8:290144/1‑141 (MQ=255)
                                                        cgcAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTc                                                                                                                                  <  5:106984/93‑1 (MQ=255)
                                                        cgcAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTc                                                                                                                                  >  6:106984/1‑93 (MQ=255)
                                                        cgcAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTaa                                                                           <  4:207102/148‑1 (MQ=255)
                                                             ttGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  <  3:306469/120‑1 (MQ=255)
                                                             ttGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGaa                                                                                                  >  4:306469/1‑120 (MQ=255)
                                                              tGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAATAGGCTCGCGGGGTGATAGaa                                                                                                  >  5:178298/1‑119 (MQ=255)
                                                              tGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAATAGGCTCGCGGGGTGATAGaa                                                                                                  <  6:178298/119‑1 (MQ=255)
                                                              tGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTa                                                                            <  5:22942/141‑1 (MQ=255)
                                                              tGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTa                                                                            >  6:22942/1‑141 (MQ=255)
                                                                cAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTaa                                                                           >  8:289741/1‑140 (MQ=255)
                                                                cAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTaa                                                                           <  7:289741/140‑1 (MQ=255)
                                                                cAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAgg                                                                   >  5:386275/1‑148 (MQ=255)
                                                                      gCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGaa                                                              <  6:386275/147‑1 (MQ=255)
                                                                           agaaACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGt                                                                                               <  1:128080/109‑1 (MQ=255)
                                                                           agaaACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGt                                                                                               >  6:25326/1‑109 (MQ=255)
                                                                           agaaACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGt                                                                                               <  5:25326/109‑1 (MQ=255)
                                                                           agaaACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGt                                                                                               >  2:128080/1‑109 (MQ=255)
                                                                                     cGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGaata                                              <  5:152680/148‑1 (MQ=255)
                                                                                                     cATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCa                                    >  7:221010/1‑142 (MQ=255)
                                                                                                     cATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCa                                    <  8:221010/142‑1 (MQ=255)
                                                                                                     cATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGtt                              >  6:241910/1‑148 (MQ=255)
                                                                                                        atGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTc                                                                      >  7:199471/1‑105 (MQ=255)
                                                                                                        atGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTc                                                                      <  8:199471/105‑1 (MQ=255)
                                                                                                        atGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGaata                                              <  2:105028/129‑1 (MQ=255)
                                                                                                        atGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGaata                                              >  1:105028/1‑129 (MQ=255)
                                                                                                        atGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGACGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCt                           >  1:88152/1‑148 (MQ=255)
                                                                                                                                  aCAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCaa  <  5:241910/147‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TATTCTGCTGGTTTCTAATGTCGCGCAAAGCTATTTCAATCAGCAACTGGCGTATGCGCAATTGCAAATAGCCGAAGAAACGCTGCGTAATTATCAGCAGTCATATGCGTTTGTCGAAAAACAACTGTTGACAGGTAGCAGTAATGTTCTGGCGCTGGAACAGGCTCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAA  >  NC_000913/596085‑596361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑