| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,021,557 | T→C | 6.5% | P444P (CCT→CCC) | ubiB → | regulator of octaprenylphenol hydroxylation, ubiquinone synthesis; regulator of 2'‑N‑acetyltransferase; putative ABC1 family protein kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,021,557 | 0 | T | C | 6.5% | 186.2 / 3.7 | 61 | P444P (CCT→CCC) | ubiB | regulator of octaprenylphenol hydroxylation, ubiquinone synthesis; regulator of 2'‑N‑acetyltransferase; putative ABC1 family protein kinase |
| Reads supporting (aligned to +/- strand): ref base T (33/24); new base C (2/2); total (35/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAGTCAAAT > NC_000913/4021412‑4021701 | tGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTgc < 7:108795/148‑1 (MQ=255) gCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGt > 1:133216/1‑147 (MQ=255) gCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGt > 4:152883/1‑147 (MQ=255) gCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCCGCGCTGGt > 3:57127/1‑147 (MQ=255) cAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTg < 2:133216/147‑1 (MQ=255) gtgtTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTa > 7:188672/1‑145 (MQ=255) tACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGaaa > 8:15921/1‑147 (MQ=255) ccAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTga < 4:180776/128‑1 (MQ=255) ccAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTga > 3:180776/1‑128 (MQ=255) ccAGAAAACCCTGCTCTACGTCGAAGGGGAAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAgcg > 6:301666/1‑148 (MQ=255) gAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCg > 1:365190/1‑148 (MQ=255) ccTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTgg > 7:188065/1‑148 (MQ=255) tGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGt > 8:82212/1‑148 (MQ=255) gTCGAAGGGGGAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGaaaaaa < 1:57233/148‑1 (MQ=255) tCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTCTGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGaaaa < 5:100399/145‑1 (MQ=255) cGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGCGCATTTAAAGAAAAAGCGCCGTTCGGGGTCGcaaaa > 6:100399/1‑145 (MQ=255) gggTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGa < 8:188672/148‑1 (MQ=255) gggTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGa < 8:188065/148‑1 (MQ=255) ggTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGaaaa < 8:128726/138‑1 (MQ=255) ggTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGaaaa > 7:128726/1‑138 (MQ=255) ggACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGcc < 6:184380/119‑1 (MQ=255) ggACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGcc > 5:184380/1‑119 (MQ=255) aCGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTACTGCGCTGGTGAGAGCATTTAAAGAAAAAGAGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCt > 7:317937/1‑148 (MQ=255) cTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTgg > 1:323581/1‑148 (MQ=255) ccGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTAc > 3:134012/1‑147 (MQ=255) cAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGaaaaa < 5:136397/117‑1 (MQ=255) cAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGaaaaa > 6:136397/1‑117 (MQ=255) aCTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGt < 3:152883/148‑1 (MQ=255) aTTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGaaa > 1:4866/1‑87 (MQ=255) aTTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGaaa < 2:4866/87‑1 (MQ=255) aTTTAGGGAAAAAGGCGAAGCCTTTCCTGGAGTCGTTGATTAAAGATCAGGTCGGTATTCCTGCGCTGGGGAGAGCCTTTAAAGAAAAAGCGCCGTTCTGGTTCGAAAAAATTCCATAACTGCCTGAATTGGTTTACTACAGTTTgcg < 8:317937/148‑1 (MQ=255) tATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGcc < 2:365190/147‑1 (MQ=255) gAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGACCTGCCTGAATTGGTTTACGACAGTTTGCGCCAggg > 5:6584/1‑147 (MQ=255) aGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTa > 5:125769/1‑146 (MQ=255) cTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATG‑CAGAACTGCCTGAATTGGTTTACGAc > 3:26877/1‑118 (MQ=255) cTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATG‑CAGAACTGCCTGAATTGGTTTACGAc < 4:26877/118‑1 (MQ=255) ttCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGca > 7:165476/1‑146 (MQ=255) ttCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGca > 6:134621/1‑146 (MQ=255) atacTGGACTAGTGGATTAAAGAAAACGTAGGTATTACCGCGATGGTGAGAGCATTTAAAGAAAAACCGCCGATCTCGCTAGAAAAAATGCCAGAACTGCCTGAATTGGTTTACTACAGTTTGCGCCAGGCCAAGTATTTACAGcaca > 8:287355/4‑148 (MQ=255) tCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGcac < 7:82212/146‑1 (MQ=255) ccTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCCGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAATAATTGCAAGAACTGCGTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAg < 7:287355/147‑1 (MQ=255) ggAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGtt > 1:47116/1‑148 (MQ=255) gAGTCGTGGATTAAAGATCAGGTCGGTATTCCCGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTg < 4:57127/148‑1 (MQ=255) tCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATa < 7:15921/148‑1 (MQ=255) gTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAg < 8:165476/148‑1 (MQ=255) tGGATTAAAGCTCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGa < 2:323581/148‑1 (MQ=255) tGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGAt > 7:115166/1‑144 (MQ=255) tGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGAt > 7:371999/1‑144 (MQ=255) tAAAGATCAGGTCGGTATTCCTGCTCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCTCCA‑GGCAAGTATTTACAGCACAGTGTTGATAAGATTGccc > 4:14479/1‑148 (MQ=255) aaaGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGcc < 8:371999/147‑1 (MQ=255) tCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAg > 3:127531/1‑148 (MQ=255) tCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGcc > 5:376265/1‑99 (MQ=255) tCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGcc < 6:376265/99‑1 (MQ=255) tCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTg > 8:103641/1‑135 (MQ=255) tCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTg < 7:103641/135‑1 (MQ=255) tCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCtt > 8:271100/1‑146 (MQ=255) cGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAg < 2:47116/148‑1 (MQ=255) tATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAGTc > 6:222058/1‑147 (MQ=255) ttCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAGTCaa < 5:301666/147‑1 (MQ=255) tCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAGTCAAAt > 1:81681/1‑148 (MQ=255) tCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAGTCAAAt > 6:226940/1‑148 (MQ=255) | TGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCCTGCGCTGGTGAGAGCATTTAAAGAAAAAGCGCCGTTCTGGGTCGAAAAAATGCCAGAACTGCCTGAATTGGTTTACGACAGTTTGCGCCAGGGCAAGTATTTACAGCACAGTGTTGATAAGATTGCCCGCGAGCTTCAGTCAAAT > NC_000913/4021412‑4021701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |