Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,700,724	A→T	I55F (ATC→TTC)	yhjV →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,700,724	0	A	T	97.0%	200.3 / NA	67	I55F (ATC→TTC)	yhjV	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (25/40);  minor base G (0/2);  total (25/42)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CAATCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGATCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTCGGGGTTATC  >  NC_000913/3700588‑3700861                                                                                                                                         |                                                                                                                                          caATCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTg                                                                                                                                     <  2:19741/143‑1 (MQ=255)  gaTCAGAAATTGGCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGc                                                                                                                                    <  1:990801/142‑1 (MQ=255)  aaTCAGAAATTGTCGTTTACACGCTACGATTTCTGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGc                                                                                                                                    <  1:374883/143‑1 (MQ=255)    tCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTAATTATTACCGCAGCGTTCATTGAtt                                                                                                                                  >  1:743447/1‑143 (MQ=255)    gcAGAAAGTGCCGTTTACACGCGACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCtt                                                                                                                                  <  2:198125/142‑1 (MQ=255)      aGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTAt                                                                                                                                <  2:695406/143‑1 (MQ=255)        aaaTTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATcc                                                                                                                              >  2:242541/1‑143 (MQ=255)          aTTGCCGTTTCCACGCTACGACTTCGGCTGGGATTTATTATGCATAGGAATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACGAATTGGCTTGTAGGGGCTTTGGGTAGTTATTGCCGCAGCGTTCGTGGCTTATCGTg                                                                                                                            >  2:572864/1‑143 (MQ=255)                gTTTCCACGCTATGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCt                                                                                                                      <  2:226422/143‑1 (MQ=255)                 tttACACGCTACGACTTCGGATGGGTTTTATGATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTg                                                                                                                     <  2:725907/143‑1 (MQ=255)                  ttACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTgg                                                                                                                    <  2:696797/143‑1 (MQ=255)                  ttACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTgg                                                                                                                    <  2:658890/143‑1 (MQ=255)                      acGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATAGCGAAAGGAACCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAAGTTGGGTATTTATTACAGCAGCGTTCATAGCACCTCCTGCCACATGGGTAg                                                                                                                >  1:638369/1‑143 (MQ=255)                           aCGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTCGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAg                                                                                                           >  2:609117/1‑143 (MQ=255)                             gccctCGGCTGGGTGTTATTACGCAGCGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTCAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGa                                                                                                         <  2:381276/139‑1 (MQ=255)                                ttCGGCTGGGTTTTTTTTTGCATTGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATAATGTCTTCTGGGTAGTGCAGGACat                                                                                                      <  1:144483/143‑1 (MQ=255)                                  cGGCTGGGTTTCATTATGCAGAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACattt                                                                                                    <  2:129958/143‑1 (MQ=255)                                    gCTGGGTTTGATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGACCGGCATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACatttat                                                                                                  <  1:292432/143‑1 (MQ=255)                                    gCTGGGTGTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACatttat                                                                                                  <  2:455004/143‑1 (MQ=255)                                        ggTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGCATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGCCATTTATTTaa                                                                                              >  2:436949/1‑143 (MQ=255)                                         gTTTTATTATGCGTAGGCATGGCGATCGGTGCCGGAACCGTGCTGAGGCGAGTACAAATTGGCTTGAAGGGAATTTGGGGATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTaaa                                                                                             <  2:478638/143‑1 (MQ=255)                                          ttttATTTCGCATAGGCTTCGCGATTTGTTTCGGAACCGTGCTGATTCCAGTACAAATTGGCTTGAAGTGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTaaaa                                                                                            <  2:161436/143‑1 (MQ=255)                                          ttttATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTaata                                                                                            >  1:419470/1‑141 (MQ=255)                                           tttATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTGGTGCAGGGCCTTTATTTaaaca                                                                                           >  1:1142922/1‑141 (MQ=255)                                            ttattaTGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCGGGACATTTATTTaacaac                                                                                          >  1:764213/1‑143 (MQ=255)                                                 atGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCttt                                                                                     <  2:1016176/143‑1 (MQ=255)                                                   ggaTAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGCACAAATTGGCTTGAAGGGAATGTGGGGAGTTATTACCGCGGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCt                                                                                   <  2:669030/141‑1 (MQ=255)                                                   gCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCCTTCt                                                                                   >  2:234971/1‑143 (MQ=255)                                                       gggcgtcgcgtgttgtgtCGGATCCGTGCTGATGCTAGTGCAAATTGGCTTGGAGGGAATTTGGGTATTTAGTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGaaa                                                                               <  1:687889/129‑1 (MQ=255)                                                         gCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTAGCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTGTCTGAAAGc                                                                             >  1:987598/1‑143 (MQ=255)                                                         gCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTGATTGCCGCAGCGTCCATTGCTTATCCTGCCACCTGGGTGGTGCCGGACATTTAGTTAAAAACCCTTGCTGACAGc                                                                             >  2:511508/1‑143 (MQ=255)                                                             ggCGTTTTGTTCCTGAACTGTTCTTATGTCATTACAAATTGGCTTGAATGGAATTTGTGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGTACATTTATTTAAAAACCCTTTCTGAAAGCGAtt                                                                         <  1:731012/143‑1 (MQ=255)                                                                gATTGGTGCCGGGTGCGTGGAGTTGCGAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCt                                                                      <  2:1090726/143‑1 (MQ=255)                                                                gATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCt                                                                      <  2:382737/143‑1 (MQ=255)                                                                gATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCGTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCt                                                                      >  1:640169/1‑143 (MQ=255)                                                                    gggacaggAGCCGTGCTTATGGCAGGACAAATTGGCTTGAAGGGCATTTGGGTATTTATTACCGCAGCGGTCATTTCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTaa                                                                  <  1:830141/137‑1 (MQ=255)                                                                    ggagCCGGAACCGTGCTGATGCCAGGAGAAAAAGGCGTGAAGGGAATTTGGGTATTTAGTACCGCAGCGTTCATTGCTTAGCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAGCCCTTTCTGAAAGCGATTCCTGTaa                                                                  <  2:502581/140‑1 (MQ=255)                                                                     gTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGGaaa                                                                 >  2:1029523/1‑142 (MQ=255)                                                                       gCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGa                                                               <  1:535764/143‑1 (MQ=255)                                                                        ccGGAACCTTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGAc                                                              <  2:1024337/143‑1 (MQ=255)                                                                        ccGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGAc                                                              >  2:1003559/1‑143 (MQ=255)                                                                        ccGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGAc                                                              >  1:450783/1‑143 (MQ=255)                                                                              ccGTGCTGATGCCAGTACAAATGGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCGCCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACAcc                                                        <  2:979414/143‑1 (MQ=255)                                                                                gTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGa                                                      <  2:1161393/143‑1 (MQ=255)                                                                                 tGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGat                                                     <  2:473605/143‑1 (MQ=255)                                                                                 tGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCGTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGat                                                     >  1:467470/1‑143 (MQ=255)                                                                                 tGCTGATGCCAGTACAAATTGGCGTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGat                                                     <  2:752339/143‑1 (MQ=255)                                                                                  gCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGata                                                    >  1:781185/1‑143 (MQ=255)                                                                                    tGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCAAtatt                                                  >  1:301626/1‑143 (MQ=255)                                                                                          cAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGt                                            >  1:1035181/1‑143 (MQ=255)                                                                                               gaaaTTGGCTTGAAGGTGAGTTGGGTATTTATTGCCGCAGCGTTCATTGCGTATCGGGCACCATGGGTAGTGCAGGACATTTATTTAAAATCCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTa                                       <  1:12495/142‑1 (MQ=255)                                                                                                gaaTTGGCGTGAAGGGAATGTGGGTAGTTATTACCGCAGCGTTCATTGCTTATCCTGCCGCCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTAc                                      <  1:349116/142‑1 (MQ=255)                                                                                                 aaTTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACAACATCGATATTATCAGTCATTAcc                                     <  1:143809/143‑1 (MQ=255)                                                                                                  aTTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTGCCCGACCTATGTCATTGCGTTGTTTGTGCAGGACATTTATTTAAAAACCCTTTCTGAACGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCt                                    <  2:848324/143‑1 (MQ=255)                                                                                                    tGGCTTGAAGGGAATTTGGGTACTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTgg                                  <  2:492585/143‑1 (MQ=255)                                                                                                        ttGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGGCATATATTTGAAAACCCTTTCTCAAAGCGATTGCTGTAATGACTAGCCCGCTACGATCAGTCATTAACTGCGgaa                              >  1:1135230/1‑143 (MQ=255)                                                                                                          gAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGgaaga                            >  1:78664/1‑143 (MQ=255)                                                                                                              ggAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCGCCCTGTGTAGTGCAGGACCTTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTg                        <  2:1101509/143‑1 (MQ=255)                                                                                                              ggAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTg                        >  2:670543/1‑143 (MQ=255)                                                                                                                aaTTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTggg                      <  1:1163746/143‑1 (MQ=255)                                                                                                                     tgttacttatgaCCGCAGCGGTTACTGCTTATCCTGCCTCCTGGGTAGTGCAGGACATTTATTTAAAATCCCTTTATGAAAGCGATTGCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAAtt                 <  1:1139809/137‑1 (MQ=255)                                                                                                                     gggTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAAtt                 <  2:888273/143‑1 (MQ=255)                                                                                                                       gTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAAtttt               <  1:670543/143‑1 (MQ=255)                                                                                                                           ttattaCCGCAGCTTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACAGTTATTTACAAATCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTc           <  1:349537/143‑1 (MQ=255)                                                                                                                           ttattaCCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGCAGAACTGGGGAATTTTCCTc           >  2:1004337/1‑143 (MQ=255)                                                                                                                                  cGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCACGGGGTTa    >  2:170140/1‑143 (MQ=255)                                                                                                                                    cAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGAGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTCGGGGTTATc  <  2:436665/143‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          CAATCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGATCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTCGGGGTTATC  >  NC_000913/3700588‑3700861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑