BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	90 [0]	[0] 90	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	87 (1.330)	71/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
*	?	NC_000913	1300698 =	0 (0.000)	87 (1.330)	71/276	0.0	100%	intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300556‑1300840

aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttgttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                <  2:266440‑M2/1‑1 (MQ=255)

   tacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                             <  2:810610‑M2/4‑1 (MQ=255)

   tacagttagttataaggatttccttaactgcttctccccgccaggtggttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                             <  2:97053‑M2/4‑1 (MQ=255)

     cagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                           <  2:777436‑M2/6‑1 (MQ=255)

      agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                          <  1:66478‑M2/7‑1 (MQ=255)

       tgtagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                         <  2:1090096‑M2/8‑1 (MQ=255)

        ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  2:188902‑M2/135‑143 (MQ=255)

            ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    >  2:67791‑M2/131‑143 (MQ=255)

            ttataaggacttccttaactgctcctcctcaccatcatgttattttcgccacatcataatcctgggctcgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  1:177219‑M2/13‑1 (MQ=255)

             tataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTg                                                                                                                                   <  2:994217‑M2/14‑1 (MQ=255)

              ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                  >  1:963920‑M2/129‑143 (MQ=255)

               taaggctttccttaactgctcctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                 <  1:1153104‑M2/16‑1 (MQ=255)

               taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                 <  1:1106541‑M2/16‑1 (MQ=255)

                aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaacgtgctaaATAATAATCAATTGTTa                                                                                                                                >  2:920104‑M2/127‑143 (MQ=255)

                       tccttaactgcgtctcacaaccatcatgttattttacctacatgatgatcctgtgcgggctgcacaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                         <  1:288443‑M2/24‑1 (MQ=255)

                          ttaactgcttctcctcgccatcatgttatgttcgccacatcataatcctgggcttgctgaagaataattgaaatgagattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTAttgta                                                                                                                      <  1:177059‑M2/27‑2 (MQ=255)

                          ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatcttcttcatcatgctgctttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                      >  2:1154553‑M2/117‑143 (MQ=255)

                          ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                      >  2:784661‑M2/117‑143 (MQ=255)

                          ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                      >  1:254082‑M2/117‑143 (MQ=255)

                              ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                  >  2:642525‑M2/113‑143 (MQ=255)

                               tgcttctcctcaccttcatgttattttcgccacatcataatcctgtgcttgctgaagaataattgaaatgatattattaattccactgcctttagtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                 <  1:174634‑M2/32‑1 (MQ=255)

                               tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                 <  1:1034086‑M2/32‑1 (MQ=255)

                                 cttctcctcaccttcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttagtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               <  1:1155187‑M2/34‑1 (MQ=255)

                                  ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                              >  2:1052822‑M2/109‑143 (MQ=255)

                                       ctcaccatcatgttattttcgccacatcatatgccgggtcgtgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                         <  1:1126109‑M2/40‑1 (MQ=255)

                                       ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                         >  1:919101‑M2/104‑143 (MQ=255)

                                        tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                        >  2:893814‑M2/103‑143 (MQ=255)

                                          accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgacatgatataattaatccccctgcctttggtagaggaaagggctaaATAATAATCAATTGTTCAATCATTGTGAATTTCACTACTGGaa                                                                                                      >  1:497762‑M2/101‑143 (MQ=255)

                                            catcatgttatttttttttcttcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGTAACt                                                                                                    >  1:597329‑M2/99‑143 (MQ=255)

                                                gtgtttttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTaa                                                                                                <  2:923597‑M2/49‑1 (MQ=255)

                                                  gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCGATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              >  2:780176‑M2/93‑143 (MQ=255)

                                                  gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              <  2:418650‑M2/51‑1 (MQ=255)

                                                  gctagtttcgccccctcctcttcctacgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              <  2:207341‑M2/51‑1 (MQ=255)

                                                   ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             <  2:457367‑M2/52‑1 (MQ=255)

                                                   tgattttcgctacatcatactcctgtgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             <  1:219186‑M2/52‑1 (MQ=255)

                                                       tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         >  2:176877‑M2/88‑143 (MQ=255)

                                                       tctcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggcagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         <  2:863422‑M2/56‑1 (MQ=255)

                                                       gttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         <  1:262059‑M2/56‑1 (MQ=255)

                                                         tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                       <  1:707917‑M2/58‑1 (MQ=255)

                                                           gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                     <  2:150159‑M2/12‑1 (MQ=255)

                                                           gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                     >  1:150159‑M2/84‑95 (MQ=255)

                                                              acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                  <  1:418989‑M2/63‑1 (MQ=255)

                                                              acatcataatcctgggctggctggagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                  >  1:896104‑M2/81‑143 (MQ=255)

                                                               cactatacgcctgggcttgctgaagaatagttgaaattatattattaattccacttcctttggtagaggaaagggctaaATAATAATCAATTGTTGAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                 <  2:233822‑M2/64‑1 (MQ=255)

                                                                atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctacATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTCTAATCAGAAAAGATAGACa                                                                                >  1:516672‑M2/79‑143 (MQ=255)

                                                                atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACa                                                                                <  2:455633‑M2/65‑1 (MQ=255)

                                                                    taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                            >  1:925377‑M2/75‑143 (MQ=255)

                                                                     aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggttgaggaaagtgctaaATAATAATCATTTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGGAAAGATAGAGATGCtt                                                                           >  1:578865‑M2/74‑143 (MQ=255)

                                                                      agcctgcgcttgccgaagaataactgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                          <  2:421846‑M2/71‑1 (MQ=255)

                                                                              cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtggtaaATAATAATCAATTGTTAAATTATTGTGCATTTAACGACTGGAACGGTAATCAGAAGAGATAGACATGATTAGCCAAtct                                                                  >  1:159016‑M2/65‑143 (MQ=255)

                                                                                 gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTa                                                               >  2:101888‑M2/62‑143 (MQ=255)

                                                                                   tgaagaataattgaaatgatattattaattccactgcgtttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTAATGGAACTGTAATCAGAAAAGATAGGCATGCTTAGTCAATCTCTAtt                                                             >  2:359575‑M2/60‑143 (MQ=255)

                                                                                   tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAGTGTATGGTGGATTGCTTAACAGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                             >  1:825864‑M2/60‑143 (MQ=255)

                                                                                    gaacaataattgaaatgacactattcactccactgccttcactgcacgaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                            <  2:486794‑M2/85‑1 (MQ=255)

                                                                                      agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                          <  1:904264‑M2/87‑1 (MQ=255)

                                                                                        aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                        <  2:420561‑M2/89‑1 (MQ=255)

                                                                                             ctgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATACTCAATTGTTAAATTCTTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAACtctctttgattcacat                                                   >  1:1111547‑M2/50‑138 (MQ=255)

                                                                                              tgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                  >  2:119159‑M2/49‑143 (MQ=255)

                                                                                                aaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                                >  1:897836‑M2/47‑143 (MQ=255)

                                                                                                 aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  2:813301‑M2/98‑1 (MQ=255)

                                                                                                   tgatattattaattccactgcttttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCATAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                             <  1:290660‑M2/100‑1 (MQ=255)

                                                                                                   tgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                             >  1:227793‑M2/44‑143 (MQ=255)

                                                                                                       attattaattccactgcttttggtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGTCATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                         <  1:999454‑M2/104‑1 (MQ=255)

                                                                                                           ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCCCTCTTTGATTGAATTGAAAGAtgtttgtt                                     >  1:360315‑M2/36‑143 (MQ=255)

                                                                                                             aatcccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACGATCGGAACTGTAATCAGAAAAGATAGACATGCCTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                   <  2:109857‑M2/110‑1 (MQ=255)

                                                                                                                  cactgcttgtggttgaggacagtgctaaAGAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCATAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGTTGTTTGTTAAGGCAt                              <  1:360502‑M2/115‑1 (MQ=255)

                                                                                                                  cactgcctttggtagatgaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  1:1037831‑M2/115‑1 (MQ=255)

                                                                                                                  cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGCAAGATGTTTGTTAAGGCAt                              >  2:228539‑M2/29‑143 (MQ=255)

                                                                                                                  cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  1:786362‑M2/115‑1 (MQ=255)

                                                                                                                   actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAATGCATg                             <  1:148092‑M2/116‑1 (MQ=255)

                                                                                                                   actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTCAATTGAAAGATGTTTGTTAAGGCATg                             >  2:110775‑M2/28‑143 (MQ=255)

                                                                                                                      gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                          <  1:26531‑M2/119‑1 (MQ=255)

                                                                                                                      gcctttagtagaggaaagtgccagATAATAATCAATTGTTAAAATATTGTGCATTTCACTACTGGAAGTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGtttgttaagcaggccgg                          <  1:1028415‑M2/119‑9 (MQ=255)

                                                                                                                        ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAGGGATGc                        >  2:1116431‑M2/23‑143 (MQ=255)

                                                                                                                          ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                      <  2:984627‑M2/123‑1 (MQ=255)

                                                                                                                          tgagtagaggaaagagctaaAGAATAATCAATTGTTAAATTAATGTGCATTTCACTACTGGAACTGTAATCATAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTCGTTAAGCCAAGCATGCaa                      <  1:837500‑M2/123‑1 (MQ=255)

                                                                                                                           tggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCGTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                     <  1:188902‑M2/124‑1 (MQ=255)

                                                                                                                           tggtagaggaaagcgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                     <  2:702925‑M2/124‑1 (MQ=255)

                                                                                                                            ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                        >  2:187430‑M2/19‑91 (MQ=255)

                                                                                                                            ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                        <  1:187430‑M2/73‑1 (MQ=255)

                                                                                                                              tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                  >  1:10810‑M2/17‑143 (MQ=255)

                                                                                                                               agaggaaagtgctaaATTATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAGCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                 <  1:174325‑M2/128‑1 (MQ=255)

                                                                                                                                gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                <  2:919101‑M2/129‑1 (MQ=255)

                                                                                                                                   gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt             >  1:656976‑M2/12‑143 (MQ=255)

                                                                                                                                    aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt            >  2:195203‑M2/11‑143 (MQ=255)

                                                                                                                                     aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  2:453157‑M2/134‑1 (MQ=255)

                                                                                                                                        tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa        >  1:386792‑M2/7‑143 (MQ=255)

                                                                                                                                          ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa      >  1:123715‑M2/5‑143 (MQ=255)

                                                                                                                                            aaATCATAATCAAGTGTTAAATTATTGTGCATTTCACTACTGTAAGTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg    <  1:346278‑M2/141‑1 (MQ=255)

                                                                                                                                              ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  2:694258/143‑1 (MQ=255)

GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300556‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`