Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,242,635 Δ1 bp coding (881/891 nt) malG ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,6350A.97.4% 156.7 / ‑3.2 39coding (881/891 nt)malGmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (21/17);  minor base C (1/0);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGCGGGTTGAGGTATTGC  >  NC_000913/4242500‑4242766
                                                                                                                                       |                                                                                                                                   
ccGGCGAACGATTCAGATCGCAGCCGAACGCACATAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCt                                                                                                                             <  1:194712/143‑1 (MQ=255)
  ggCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACACCTTCACCCCTTC‑CACCACCTGc                                                                                                                           >  1:208325/1‑143 (MQ=255)
        cGATTCAGATTGCAGACGCAAGAAAAAAAGGCGCTCCGTGGAGCCCCGAATAACAGTCACAAGTTGGGATACCGTAAGGTGAGGGTGCAGCGGCACAACCTGGGCAGAAACACACCGTTCGCCTTTC‑CACCCCCTGCCGTCAg                                                                                                                     >  2:91107/1‑143 (MQ=255)
          aTTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGACGCTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGc                                                                                                                   <  1:103192/143‑1 (MQ=255)
           ttCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCAGGACAGTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCGGGct                                                                                                                  >  1:412482/1‑142 (MQ=255)
            tCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCg                                                                                                                 >  1:666768/1‑143 (MQ=255)
              aGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTACCCTTTC‑CACCACCTGCCGTCAGGCCGtt                                                                                                               >  1:259797/1‑143 (MQ=255)
                   gCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTCCGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTACCGACAGACCGTTGAcca                                                                                                          >  1:685499/1‑143 (MQ=255)
                     aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGAccagc                                                                                                        >  2:328420/1‑143 (MQ=255)
                     aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGATGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTCTAACCTTTC‑CACCACCTGCCGTCAGGCCgctaccccgc                                                                                                        >  2:441633/1‑134 (MQ=255)
                         gaaagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCCGAACCACACCTGTCACC‑GTCCCACCACCTGCCGTCACGCCGTTGACcagccagc                                                                                                    >  2:564842/1‑143 (MQ=255)
                           aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGt                                                                                                  >  1:111310/1‑143 (MQ=255)
                           aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAAAGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCGGTTGACCAGCCAGCGt                                                                                                  >  1:32625/1‑143 (MQ=255)
                            agaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTGTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGtt                                                                                                 >  1:233081/1‑143 (MQ=255)
                              aaaaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCGCGCGTTGa                                                                                               >  1:674053/1‑143 (MQ=255)
                              aaaaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGa                                                                                               <  1:215239/143‑1 (MQ=255)
                                aaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGc                                                                                             <  2:142936/143‑1 (MQ=255)
                                          tCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCaagaag                                                                                   >  1:434854/1‑143 (MQ=255)
                                             gTGGACCTCCGAATAACATGCACAAGAGGAAATAACGTAACTTGAGGGTTCAGCGGCATAACAGTGGCAGAACAACCTCTCTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACg                                                                                <  2:147308/143‑1 (MQ=255)
                                                  gcgcGGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt                                                                           <  2:203949/143‑1 (MQ=255)
                                                   cgcggaagaacgtgcacaACGTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTg                                                                          <  2:25854/129‑1 (MQ=255)
                                                   cgcCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGTAAGAAGACGATGGTg                                                                          <  1:469324/143‑1 (MQ=255)
                                                     ccGAATAACAGTCTAGTGTGTGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGAt                                                                        <  2:470382/143‑1 (MQ=255)
                                                     ccGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGAt                                                                        >  1:345389/1‑143 (MQ=255)
                                                       gAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCg                                                                      <  1:702238/143‑1 (MQ=255)
                                                          tAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTGTAACCGTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTa                                                                   <  2:461665/143‑1 (MQ=255)
                                                               gTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCa                                                              <  1:595439/143‑1 (MQ=255)
                                                                  acaAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCGTTC‑CACCACCTGCCGTCGGGGCGTGGACCAGCCAGCGTTGAGCCAGCGCGAAGACGATGTTGCTCGGTAATGCAGAc                                                           >  1:333668/1‑143 (MQ=255)
                                                                                   aaGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCGGGCATCACGGCAGCggcggc                                          >  1:616520/1‑143 (MQ=255)
                                                                                       gtaGGGGGCAGCGGCGTACCGTTGGCAGAGCAACATCTTTAACCTTTC‑CGCCACCGGCCGTCAGGCCGTCGTCCAGCCAGCGTTGATCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAg                                      <  2:314357/141‑1 (MQ=255)
                                                                                             ggCAGCGGCATAACCTTGGCAGCACGCCATCCTTAACCTTTC‑CACCCCCTGCCGTCAGGCCGTTGACCACCCAGCTTTGAGCCAGCAAGCAGCCGATGGTGATCTGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCAccc                                <  2:599084/142‑1 (MQ=255)
                                                                                                     cATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGGCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAGTGAAGACATCGTGGCAGCGGCGGCAAAGTCACCGCACAGGtg                        >  1:270702/1‑142 (MQ=255)
                                                                                                     cATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTa                        <  2:62609/143‑1 (MQ=255)
                                                                                                            ttGGCAGAACTACATCTTTAACCTTTC‑CAACACCCGCCGTCAGGTACCAGAGGTGCCGTGTTTGTGCCCGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGc                 <  2:153055/143‑1 (MQ=255)
                                                                                                                    acaacaTCTTTACCCTTTC‑CACCGCCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGCggcctaag         <  2:126625/143‑7 (MQ=255)
                                                                                                                     caacaTCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGGCCACCCGCAGGGAGTTTTGGGGGGTgggg        >  2:577519/1‑143 (MQ=255)
                                                                                                                     caacaTCTGTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGGAAAGTCACCCCACAGGCGGGCTTGCGGGATGAgg        >  2:719934/1‑143 (MQ=255)
                                                                                                                      aacaTCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTAGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCCGCAAAGCCCCCCCAAAGGTAGATTTGCGGGCTCAgga       >  2:64887/1‑142 (MQ=255)
                                                                                                                           cTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTAACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCGGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGGGGTTGGAgggttggc  >  1:225171/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGCGGGTTGAGGTATTGC  >  NC_000913/4242500‑4242766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑