Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,773,068 A→G 8.3% pseudogene (113/117 nt) yfjV ← pseudogene, CP4‑57 prophage,Phage or Prophage Related, putative pump protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,773,0680AG 8.3% 150.7 / 4.6 48pseudogene (113/117 nt)yfjVpseudogene, CP4‑57 prophage,Phage or Prophage Related, putative pump protein
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base G (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

CCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAG  >  NC_000913/2772780‑2773347
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                       
ccTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATACTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCa                                                                                                                                                                                                                                                                                         >  2:150884/1‑289 (MQ=255)
ccTGAAGGTTGCCAGAGCCTGAATCGAACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCa                                                                                                                                                                                                                                                                                         <  1:176209/289‑1 (MQ=255)
              gATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGAt                                                                                                                                                                                                                                                                                  >  2:8561/1‑282 (MQ=255)
              gATCCTTGATCGCACTGGCAAGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGAt                                                                                                                                                                                                                                                                                  <  1:8561/282‑1 (MQ=255)
                         gCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAAt                                                                                                                                                                                                                                                                <  2:159014/289‑1 (MQ=255)
                           aCTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCg                                                                                                                                                                                                                                                              <  2:68672/289‑1 (MQ=255)
                                                 gAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCgcggcg                                                                                                                                                                                                                                          <  2:213243/287‑1 (MQ=255)
                                                 gAAACTTAATACGTGGCCGGAAAGATGCTGCGAAGGAACGGATACGGCATAAGCACCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACGGCGAAAAGCTCAGACCGAAGAAGGCCGCCGGGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCgcggcg                                                                                                                                                                                                                                          <  1:165997/287‑1 (MQ=255)
                                                               tGCCGGAATAACTCTGCGAAAGAAGGGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaac                                                                                                                                                                                                                           <  1:292873/288‑1 (MQ=255)
                                                               tGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaaca                                                                                                                                                                                                                          >  2:292873/1‑289 (MQ=255)
                                                                gCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAACGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCTAGCACCATCGGCGACAGAATCAGAGCGGCGCCTTCATTGGTAtacaa                                                                                                                                                                                                                         >  1:81810/1‑289 (MQ=255)
                                                                           tctGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAg                                                                                                                                                                                                              <  2:294790/289‑1 (MQ=255)
                                                                                              aGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaac                                                                                                                                                                                                                           >  1:403518/1‑257 (MQ=255)
                                                                                              aGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAaac                                                                                                                                                                                                                           <  2:403518/257‑1 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATACATCCTGCAGCCATGACAAAGGCCAGTGTCGTTCCCTGGCTGAACCCAGTGCGATCAGCATAGCAATCAAAAAGCGCGCCAGGTACCGCGGGCGCCGTAATTGGCAAACAACGCGTCACAAGGGACACCGAGACAGGCTTTCCAgggctacagc                                                                                                                                                                              >  1:191779/1‑281 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAAt                                                                                                                                                                                                                                                                <  2:139745/208‑1 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAAt                                                                                                                                                                                                                                                                >  1:139745/1‑208 (MQ=255)
                                                                                                          gTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGACCAGCATCGCAATCACAATCGGCCTCAGGATCAGCTCGGCGCCGTCATTTGCAAACAACGCATCAACCGCCGCACCACGCAAGACtttaccggcaaaaa                                                                                                                                                                                >  1:218343/1‑277 (MQ=255)
                                                                                                             gCCGCAAGCGCGGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAgcg                                                                                                                                                                             <  1:377072/288‑1 (MQ=255)
                                                                                                               cGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGACAGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGct                                                                                                                                                                           <  1:48969/288‑1 (MQ=255)
                                                                                                                      gCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGctc                                                                                                                                                                          <  2:133518/282‑1 (MQ=255)
                                                                                                                                ccACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCCAGACTTTCCAGGTGAACAGCGACCTAGGTAGGAAcctgtc                                                                                                                                                          >  1:133518/1‑283 (MQ=255)
                                                                                                                                 agtgagtaagggccgggAGGCGGACTGCGAAAAGCTCAGACCGAAGAGGTCCGCCGAGACGATATTAACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACTTATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGt                                                                                                                                                          <  1:192201/272‑1 (MQ=255)
                                                                                                                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACCGCGCTCtaggt                                                                                                                                                                     >  1:277540/1‑267 (MQ=255)
                                                                                                                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCtaggt                                                                                                                                                                     <  2:277540/267‑1 (MQ=255)
                                                                                                                                                ggAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCATGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCCAGGTAGGAACTTTGTCGTTGATAGAGTCTTCa                                                                                                                                         <  2:439766/289‑1 (MQ=255)
                                                                                                                                                       tACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCAt                                                                                                                                    >  1:496612/1‑287 (MQ=255)
                                                                                                                                                       tACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCAt                                                                                                                                    <  2:496612/287‑1 (MQ=255)
                                                                                                                                                          tGCGGAAAGCTCCGACCGACGAAGTCCGCCGGGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAaca                                                                                                                               <  1:60111/289‑1 (MQ=255)
                                                                                                                                                            cgcAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACTTATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATagc                                                                                                                                                >  2:192201/1‑269 (MQ=255)
                                                                                                                                                                             agaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTg                                                                                                                                                     <  2:44983/248‑1 (MQ=255)
                                                                                                                                                                             agaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTg                                                                                                                                                     >  1:44983/1‑248 (MQ=255)
                                                                                                                                                                                                                aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCaa                                                                                                                                                                                                 <  1:447077/169‑1 (MQ=255)
                                                                                                                                                                                                                aCAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCaa                                                                                                                                                                                                 >  2:447077/1‑169 (MQ=255)
                                                                                                                                                                                                                    tGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGctc                                                                                                                                                                          >  1:70688/1‑188 (MQ=255)
                                                                                                                                                                                                                    tGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCGGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGctc                                                                                                                                                                          <  2:70688/188‑1 (MQ=255)
                                                                                                                                                                                                                       gCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGTCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCCCTAGGTAGGAACTTTGTCGTGGATCGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAACTTGGCTGCTAGAAAGTCGATGATTTTTTGCGTATGATTaaaaa                                                                  >  2:244959/1‑289 (MQ=255)
                                                                                                                                                                                                                        cGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAac                                                                                                                                <  1:343617/226‑1 (MQ=255)
                                                                                                                                                                                                                        cGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAac                                                                                                                                >  2:343617/1‑226 (MQ=255)
                                                                                                                                                                                                                                tGGCCGTATCTGCAATACATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTa                                                         <  2:81810/289‑1 (MQ=255)
                                                                                                                                                                                                                                       aTCGGCCATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCt                                                   <  1:32473/288‑1 (MQ=255)
                                                                                                                                                                                                                                                  aTCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGAt                                       <  2:218343/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACt                                                                                                                                                              <  2:426990/166‑1 (MQ=255)
                                                                                                                                                                                                                                                      tGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACt                                                                                                                                                              >  1:426990/1‑166 (MQ=255)
                                                                                                                                                                                                                                                        cAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGCAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATAAATGGATTTGCTa                                 >  1:454210/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           tgtCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCt                                                   <  2:105637/252‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tgtCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCt                                                   >  1:105637/1‑252 (MQ=255)
                                                                                                                                                                                                                                                                                tGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGaaa         >  1:18489/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     tGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTa    >  2:161486/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                       gCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTaag  >  2:328643/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                       
CCTGAAGGTTGCCAGATCCTTGATCGCACTGGCAGGCGTCTTCAGCAGCGAAACTTAATACGTTGCCGGAATAACTCTGCGAAAGAAGAGATACAGCATAATCAGCGTGGCCGCAATCGCTGCCGCATCCACGGAGATCATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAGTGTCGTGCCCTGGCTGAACCCAGTGCGATCAGCATCGCAATCACAATCGGCGTCAGGATCAGCGCGGCGCCGTCATTGGCAAACAACGCATCAACAGCGGCACCGAGCAAGACTTTCCAGGTGAACAGCGCTCTAGGTAGGAACTTTGTCGTTGATAGAGTCTTCAGCCATCAACACTGTATCGAGTAATGAAATTGGTTGCTAGAAAGTCGATGATTTGTTGCGTATGATTAAAAATCAACCTTAAAGGCTTTATACATGGATTTGCTATGGCTTTTGTATCAGAAAGAGAAATTGTAAG  >  NC_000913/2772780‑2773347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: