Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,677,913 T→C 100% intergenic (‑10/‑514) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,677,9130TC100.0% 187.8 / NA 51intergenic (‑10/‑514)pntA/ydgHpyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/28);  total (23/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACTCACCAGCGTTGTCCCAGGATTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACGGTCGGGTAAAACGAGACATCGCCCCGGCACGAAT  >  NC_000913/1677630‑1678195
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                          
caCTCACCAGCGTTATCCCAAGATTCAATAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATAATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGATGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCAt                                                                                                                                                                                                                                                                                       <  1:308149/288‑1 (MQ=255)
 aCTCACCAGCGTTGTCCCAGGATTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCAt                                                                                                                                                                                                                                                                                       >  1:27081/1‑288 (MQ=255)
 aCTCACCAGCGTTGTCCCAGGATTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATc                                                                                                                                                                                                                                                                                      >  2:57459/1‑289 (MQ=255)
      ccAGCGTTGTCCCAGGATTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGttt                                                                                                                                                                                                                                                                                 >  2:391999/1‑289 (MQ=255)
      ccAGCGTTGTCCCAGGATTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGttt                                                                                                                                                                                                                                                                                 >  2:260231/1‑289 (MQ=255)
                      aTTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGt                                                                                                                                                                                                                                                                    <  2:27081/286‑1 (MQ=255)
                         cAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTg                                                                                                                                                                                                                                                                 >  2:346713/1‑286 (MQ=255)
                         cAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCt                                                                                                                                                                                                                                                              <  2:188549/289‑1 (MQ=255)
                              aCGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTc                                                                                                                                                                                                                                                         <  2:363746/289‑1 (MQ=255)
                              aCGCAATTTCATCATCGAACGGCGCATTGACCTTCAGAAGGATCTCTGACTGCCAGACGCTATTCCCGTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTGATCGTCAAAACGTGCCAGTTGACCCGCGCCGCTCGCGACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTc                                                                                                                                                                                                                                                         <  2:425702/289‑1 (MQ=255)
                               cGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCCCAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTTGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCTATTCGCATGATATTCCCCTCCCTCGGTTTCATTGATGATGGTTTGCCTGTGTc                                                                                                                                                                                                                                                         >  2:336155/1‑288 (MQ=255)
                               cGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCAt                                                                                                                                                                                                                                                                                       >  1:103823/1‑258 (MQ=255)
                               cGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCAt                                                                                                                                                                                                                                                                                       <  2:103823/258‑1 (MQ=255)
                                  aaTTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGa                                                                                                                                                                                                                                                     <  2:328018/289‑1 (MQ=255)
                                                aCGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAgctg                                                                                                                                                                                                                                        >  1:272528/1‑288 (MQ=255)
                                                    cgcATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATtgat                                                                                                                                                                                                                                                                          <  2:133903/250‑1 (MQ=255)
                                                    cgcATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATtgat                                                                                                                                                                                                                                                                          >  1:133903/1‑250 (MQ=255)
                                                      cATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATCTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCCAACCAGCTGCTCATGt                                                                                                                                                                                                                                 >  1:347191/1‑289 (MQ=255)
                                                       aTTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATCTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTa                                                                                                                                                                                                                                <  2:347191/289‑1 (MQ=255)
                                                                          tctGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcaca                                                                                                                                                                                                                                               <  2:235059/255‑1 (MQ=255)
                                                                          tctGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCcaca                                                                                                                                                                                                                                               >  1:235059/1‑255 (MQ=255)
                                                                          tctGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCg                                                                                                                                                                                                             >  1:195831/1‑289 (MQ=255)
                                                                             gACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTa                                                                                                                                                                                                          >  2:314062/1‑289 (MQ=255)
                                                                                               cctgccaaactttCACCGCCCGCTTGCACCAACGCTCTATCGTCAAAACTTGCCAGTGGACGCGCGCCTCTCTCTACCTCGACGGTAAAACCCCGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTATCCTTTCTCTTGGTATGCCAATTCGCATGATATTCCCCGCCATCGGCTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTGATATACGAGGTAAATGTGACGCCGTTAAAATAAATTAGTAACaaa                                                                                                                                                                                        <  1:45880/279‑1 (MQ=255)
                                                                                               ccTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaaa                                                                                                                                                                                        <  1:57459/289‑1 (MQ=255)
                                                                                               ccTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaaa                                                                                                                                                                                        <  1:260231/289‑1 (MQ=255)
                                                                                                 ttccaagcgtcccgcccccGCTTGCCCAAACCCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACg                                                                                                                                                                                      <  1:346713/273‑1 (MQ=255)
                                                                                                  tCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACg                                                                                                                                                                                      >  2:251259/1‑288 (MQ=255)
                                                                                                  tCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACg                                                                                                                                                                                      >  2:77915/1‑288 (MQ=255)
                                                                                                   cTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGcc                                                                                                                                                                                    <  1:251259/289‑1 (MQ=255)
                                                                                                    tACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTa                                                                                                                                                                                                                                                                               >  1:67440/1‑197 (MQ=255)
                                                                                                    tACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTa                                                                                                                                                                                                                                                                               <  2:67440/197‑1 (MQ=255)
                                                                                                          ttCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAAc                                                                                                                                                                             <  1:314062/289‑1 (MQ=255)
                                                                                                                gcCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACt                                                                                                                                                                        <  1:387567/288‑1 (MQ=255)
                                                                                                                          acaAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGc                                                                                                                                                             <  1:77915/289‑1 (MQ=255)
                                                                                                                                          cAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCa                                                                                                                                             <  2:272528/289‑1 (MQ=255)
                                                                                                                                                                               gACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAAc                                                                                                                                                                                       <  2:157604/210‑1 (MQ=255)
                                                                                                                                                                               gACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAAc                                                                                                                                                                                       >  1:157604/1‑210 (MQ=255)
                                                                                                                                                                                   gTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGAt                                                                                                    >  2:468823/1‑289 (MQ=255)
                                                                                                                                                                                    tAAAACCCAGTTTCAGCGGCGTTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGAt                                                                                                    <  1:373649/288‑1 (MQ=255)
                                                                                                                                                                                               ttCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATa                                                                                        <  2:195831/289‑1 (MQ=255)
                                                                                                                                                                                                    cagcTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAACTATTAACGATTCAGCCGTGATa                                                                                        >  1:157952/1‑284 (MQ=255)
                                                                                                                                                                                                    cagcTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATa                                                                                        <  2:157952/284‑1 (MQ=255)
                                                                                                                                                                                                                       ggCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCt                                                                                                                                                                                                                          >  2:231038/1‑135 (MQ=255)
                                                                                                                                                                                                                       ggCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCt                                                                                                                                                                                                                          <  1:231038/135‑1 (MQ=255)
                                                                                                                                                                                                                                               ttGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTc                                        <  2:189191/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACgg                                   >  1:387683/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                         aaTTCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACGGTCGGGTAAAACGAGACATCg               >  1:83162/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                           ttCGCATGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACGGTCGGGTAAAACGAGACATCGcc             >  1:15311/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                aTGATATTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACGGTCGGGTAAAACGAGACATCGCCCCGGCa       <  2:83162/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     atTCCCCTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACGGTCGGGTAAAACGAGACATCGCCCCGGCACGAAt  <  2:387683/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                          
AACTCACCAGCGTTGTCCCAGGATTCAGTAACGCAATTTCATCATCTAACGGCGCATTGACCTTCAGAATGATCTCTGACTGCCAGACGCTATTCCCTTCTACAATTTCAGCGCCCGCTTGCACAAACGCTTTATCGTCAAAACTTGCCAGTTGACCCGCGCCGCTCTCTACCGCGACGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAATCAGTATTTCGGCGTGAACTCGCAAAATATTAACGATTCAGCCGTGATAGTGGGATAAACACCTTAGAACGCCGGATAAAGACTGATAATTGTCTTCGACGGTCGGGTAAAACGAGACATCGCCCCGGCACGAAT  >  NC_000913/1677630‑1678195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: