Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,466,438	T→G	20.4%	pseudogene (1047/2513 nt)	ydbA →	pseudogene, autotransporter homolog, interrupted by IS2 and IS30

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,438	0	T	G	20.4%	58.2 / 11.6	34	pseudogene (1047/2513 nt)	ydbA	pseudogene, autotransporter homolog, interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (13/14);  new base G (2/5);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

GCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACG  >  NC_000913/1466173‑1466705                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                             ggggcggTCACGGCATTGATATCACCGATGACAGCGCGACGGTGGATAACAAGGGCACCATAACCGTCACCGATCCGTAGTCCATGGGTATCAAGATCGACGGTGGCAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGGGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGa                                                                                                                                                                                                                                                                      <  1:317534/272‑1 (MQ=255)                        aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATCACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGCAAAGTTATTCAGGACGGCGATCTGGAAGACAgcggcgg                                                                                                                                                                                                                                              >  1:303119/1‑275 (MQ=255)                        aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                                                                                                                                              >  1:233357/1‑275 (MQ=255)                        aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                                                                                                                                              >  1:245758/1‑275 (MQ=255)                          cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                                                                                                                                                            >  2:351571/1‑275 (MQ=255)                             tGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTAAGGACGGCGATCTGGATGTCAGCGGCGGCGGTc                                                                                                                                                                                                                                         >  1:254778/1‑275 (MQ=255)                              gagtgcgcGTAGGTGGATATAAAGGGCACAACGACCGTCAGACATCCGTAGGTGATGTGTATCCAGAGCGACGGGAACAACGGAATCGTCACTGACGACGCCGGGCGGACCAGTACGAAGTGTGGAACAGGCACGGAGATTACTGGTGATAATGCCACAGTAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                                        <  2:410651/271‑1 (MQ=9)                              gACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                                        <  2:245758/275‑1 (MQ=255)                              gACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                                        <  2:233357/275‑1 (MQ=255)                                         ggTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                                                                                                                                             <  1:351572/275‑1 (MQ=255)                                         cgtggtaaaCCAGGGCACCAGGCCCGTCACCCATCCGGAGTCCATTGGTATCCAGGTCGACGTTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCAACACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                                                                                                                                             <  2:254778/268‑1 (MQ=17)                                                     gggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTACACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACa                                                                                                                                                                                                                >  1:511450/1‑275 (MQ=255)                                                       gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAg                                                                                                                                                                                                               >  1:448672/1‑274 (MQ=255)                                                             tGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTACACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACg                                                                                                                                                                                                        <  2:511449/275‑1 (MQ=255)                                                                   tCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATAACCAACGGTGGCACCGGCACCAAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTTGATAGGTACGAAAATAAACGGTAATAACGGAAAAGTTATTAAGGACGGCGATCTGGATGTCAGCGGCGGCGGTTAAGGTATTGATA‑TAACCGGCGACAGGGCAACGGTGGAt                                                                                                                                                                                                  >  1:198506/1‑275 (MQ=17)                                                                           ccGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACa                                                                                                                                                                                              >  1:199729/1‑271 (MQ=255)                                                                               aGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAggg                                                                                                                                                                                          <  2:199729/271‑1 (MQ=255)                                                                                         tggaaaagagacaggggACAAGGCCATCGTCAAAAACAAAGGCGGGAGCACCACAACCAACGGTGGCACCGGCACCCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGACGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCa                                                                                                                                                                            <  2:110298/259‑1 (MQ=11)                                                                                          tgggctcggagaagtcgataagagacagTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCCCCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCGGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCAc                                                                                                                                                                           <  1:204064/248‑1 (MQ=14)                                                                                               aaTCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                                                                                                                                             <  2:323828/220‑1 (MQ=255)                                                                                                                ccATCCTCAATAACAAAGTCAAGAGCACCATCACCAACGGTTGCACCGTCACCCAGATTAATGGTGATAACGCCACGTCAAACAACAACGGCAAAACCACCTTTTACGGCAATGATTCCACCGTTACGTAAATCAACGGTAATAACGGAAAATTTATTCAGTACGGCGATCTATATGTCAGCCGCCGCGATCACCGTATTGATA‑TCACCCGCCACAGCCAAACGTTTGATAACAAGAGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATc                                                                                                                                                     <  2:198506/275‑1 (MQ=14)                                                                                                                  aTCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGCCGACCGGTATCCa                                                                                                                                                   >  1:110298/1‑275 (MQ=255)                                                                                                                           aaCGAAGGCGCGAACACCAACACCAACGATGGCGCCGGCACCCATAGTAATGGTGATAAGGCCACAGAAAACAACAACGGCAAAACCACCGTTGACGGAAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACgg                                                                                                                                          <  1:289443/275‑1 (MQ=14)                                                                                                                           aaCGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCAATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGa                                                                                                                                                                        >  1:468048/1‑245 (MQ=11)                                                                                                                               aaGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCAATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                                                                                                    <  2:468047/245‑1 (MQ=11)                                                                                                                                 ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCg                                                                                                                                                                               >  2:204064/1‑232 (MQ=255)                                                                                                                                              tCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAAGCCATCGGCATCCAGATTGAAGGCGACCAGGCCgttgtaca                                                                                                                        >  1:270188/1‑271 (MQ=255)                                                                                                                                                          gcaccggcaccCAGATTGATGGGGAGGACGCCACGGCAAACAACAACGGCAAAACCACCGGTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAGAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGTCGACGGTCGGGAGAGTCAGATG‑GGTAGAGGAAAGATGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCgag                                                                                                           <  2:448671/275‑1 (MQ=17)                                                                                                                                                                                 ggtgaCGCCACGGGAAACAACAACGTCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAGCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCa                                                                                     <  2:499544/272‑1 (MQ=255)                                                                                                                                                                                  atgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCTCACCGGTGGCTAACAAGGGCACCATGACCGTCATCGATCCTGATTCCATCGGTATCAAGGTTGACGGCGACCAGGCGGTTGTTAACAACGCAGGCGAGAGCGCCATCACCAACGGt                                                                                         >  1:499545/1‑269 (MQ=255)                                                                                                                                                                                      tgCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGTGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCa                                                                               <  2:253150/274‑1 (MQ=255)                                                                                                                                                                                                                                       gccGGGACGGAAATCAACGGGAATAACGGAAAAGTGATGCAGGACGGCGGTCTGGATGTGGGCGGGGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAacca                               <  2:359129/273‑1 (MQ=255)                                                                                                                                                                                                                                                           taataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                                                                                                                                                                                                                                        <  2:356732/54‑1 (MQ=255)                                                                                                                                                                                                                                                                   gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCGATCACCAACGGTGGAACCGGCACGCAGATAAACGGTGATGACGCCACGGCAAACCACAACGTAAAAAACACAGTTGACGGCAAGGGTTACACCGGAACg  >  1:511349/1‑275 (MQ=17)                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                             GCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATA‑TCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACG  >  NC_000913/1466173‑1466705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑