BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,556,097	Δ1 bp	2.9%	coding (225/831 nt)	yjiC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,097	0	T	.	2.9%	515.6 / 4.5	138	coding (225/831 nt)	yjiC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (60/74); new base . (2/2); total (62/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 138 total aligned reads displayed.

TCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAA  >  NC_000913/4555827‑4556361

tCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGcc                                                                                                                                                                                                                                                                      >  1:282155/1‑275 (MQ=255)

tCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAAAAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGCTTTTCAAGTTCCGATATAGCATACGTCCTGTTGTTGTAATACCTTCGGATTCTCTTCCTGCTCCTGTCACATAATTAAATggac                                                                                                                                                                                                                                                                      >  1:110500/1‑273 (MQ=255)

   tATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAg                                                                                                                                                                                                                                                                   >  2:294820/1‑275 (MQ=255)

   tATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAg                                                                                                                                                                                                                                                                   >  2:81568/1‑275 (MQ=255)

   tATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCCCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAg                                                                                                                                                                                                                                                                   >  1:281934/1‑275 (MQ=255)

         ggTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCa                                                                                                                                                                                                                                                                     <  2:151207/267‑1 (MQ=255)

                          gAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGtttt                                                                                                                                                                                                                                            >  2:176649/1‑275 (MQ=255)

                                tGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAAtt                                                                                                                                                                                                                                      >  2:214002/1‑275 (MQ=255)

                                tGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGAATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAAtt                                                                                                                                                                                                                                      >  2:422879/1‑275 (MQ=255)

                                        gggCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAAATCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGAATACGTCCTGTTGTTGTAATACCATCGGATTATCTGCATGCCCCTTTCACTTTATTACATGGCCAAGGCTCTTTACTACTCCTAAGTTCTCCAATTTCaacaac                                                                                                                                                                                                                              >  1:91308/1‑275 (MQ=255)

                                         ggCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACa                                                                                                                                                                                                                                <  2:207617/272‑1 (MQ=255)

                                               aTGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCttttt                                                                                                                                                                                                                       >  1:191141/1‑275 (MQ=255)

                                                 gtgaTTTCAGGAACTTGATAGTCCTAAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTTAACCTTCTGCTAACTGTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTACAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTcc                                                                                                                                                                                                                     <  1:509601/272‑1 (MQ=255)

                                                   tATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGTCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGCTCCGATAGAGCATACGTCCTGTTGGTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCAt                                                                                                                                                                                                                   <  1:49941/275‑1 (MQ=255)

                                                   tATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCAt                                                                                                                                                                                                                   <  1:228752/275‑1 (MQ=255)

                                                   tATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCAt                                                                                                                                                                                                                   >  1:37925/1‑275 (MQ=255)

                                                        cATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCg                                                                                                                                                                                                                 <  1:6400/272‑1 (MQ=255)

                                                                   taGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTAtt                                                                                                                                                                                                   >  2:19074/1‑275 (MQ=255)

                                                                      tCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTc                                                                                                                                                                                                                                    >  2:496635/1‑239 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCttttt                                                                                                                                                                                                                       >  2:322023/1‑247 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTAtt                                                                                                                                                                                                   >  2:496825/1‑267 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAg                                                                                                                                                                                           >  2:426082/1‑275 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAg                                                                                                                                                                                           >  1:154958/1‑275 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAg                                                                                                                                                                                           >  2:112804/1‑275 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAg                                                                                                                                                                                           >  2:510076/1‑275 (MQ=255)

                                                                           aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAA‑‑GGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTa                                                                                                                                                                                         >  2:8551/1‑275 (MQ=255)

                                                                               ggATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCAt                                                                                                                                                                                                                   <  1:322023/247‑1 (MQ=255)

                                                                               ggATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCa                                                                                                                                                                                               <  1:496826/267‑1 (MQ=255)

                                                                                gATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGCGTAaga                                                                                                                                                                                      >  1:79803/1‑275 (MQ=255)

                                                                                gATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAaga                                                                                                                                                                                      >  2:40709/1‑275 (MQ=255)

                                                                                  tCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAgg                                                                                                                                                                                    <  2:37925/275‑1 (MQ=255)

                                                                                         ggCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAaga                                                                                                                                                                             >  2:488812/1‑275 (MQ=255)

                                                                                                cATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGt                                                                                                                                                                      >  1:191113/1‑275 (MQ=255)

                                                                                                        aatTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGtt                                                                                                                                                                     >  2:164671/1‑268 (MQ=255)

                                                                                                               ccGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGc                                                                                                                                                       >  2:73976/1‑275 (MQ=255)

                                                                                                                cGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAA‑‑GGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTg                                                                                                                                                    <  1:8551/275‑1 (MQ=255)

                                                                                                                       cTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCatat                                                                                                                                               <  1:488813/275‑1 (MQ=255)

                                                                                                                              tAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAAtt                                                                                                                                        <  2:191113/275‑1 (MQ=255)

                                                                                                                                 ctcgGTGGTAGCATTCGCCTTTTTACATATGCCACCATATATTTCGTCTTAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGGTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCTGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTa                                                                                                                                      <  2:410878/274‑1 (MQ=255)

                                                                                                                                     gtgggagcagtggcCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACgtgt                                                                                                                                 <  2:9541/263‑1 (MQ=255)

                                                                                                                                       ggTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTaa                                                                                                                               >  2:46717/1‑275 (MQ=255)

                                                                                                                                          aGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACt                                                                                                                            <  1:510077/275‑1 (MQ=255)

                                                                                                                                          aGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACt                                                                                                                            <  1:182130/275‑1 (MQ=255)

                                                                                                                                           gCATGCGCCTTTGTACATATACCACCATATATTTCGGCTAAAGCTGAGTTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACt                                                                                                                            <  2:134357/274‑1 (MQ=255)

                                                                                                                                            cATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCa                                                                                                                          <  1:112804/275‑1 (MQ=255)

                                                                                                                                              tGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACt                                                                                                                        >  2:131525/1‑275 (MQ=255)

                                                                                                                                                cgcCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCg                                                                                                                                                                >  2:270109/1‑233 (MQ=255)

                                                                                                                                                cgcCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATg                                                                                                                                                        >  2:164940/1‑241 (MQ=255)

                                                                                                                                                   cTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACt                                                                                                                            >  2:371767/1‑266 (MQ=255)

                                                                                                                                                   cTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGa                                                                                                                   <  1:46717/275‑1 (MQ=255)

                                                                                                                                                    tttttACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATc                                                                                                                                                            <  1:270109/233‑1 (MQ=255)

                                                                                                                                                    tttttACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTg                                                                                                                                                    <  1:164940/241‑1 (MQ=255)

                                                                                                                                                    tttttACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTg                                                                                                                                                    <  1:325812/241‑1 (MQ=255)

                                                                                                                                                      tttACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAaga                                                                                                                                                                                      >  2:65383/1‑205 (MQ=255)

                                                                                                                                                      tttACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCg                                                                                                                                                      >  2:122500/1‑237 (MQ=255)

                                                                                                                                                       ttACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACt                                                                                                                        <  1:371768/266‑1 (MQ=255)

                                                                                                                                                        tACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTAt                                                                                                               <  1:294820/274‑1 (MQ=255)

                                                                                                                                                        tACATATGCCACAATATAATTCGTCTAAAGCTGAGGTGAATTTCAAGTTCCGATAGAGCATACGTCCTGTTTTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTAtt                                                                                                              <  1:433007/275‑1 (MQ=255)

                                                                                                                                                          cATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCg                                                                                                                                                                                  <  1:65383/205‑1 (MQ=255)

                                                                                                                                                          cATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCa                                                                                                                                                  <  1:122500/237‑1 (MQ=255)

                                                                                                                                                           atatGCCACCATATATTTCGTCTAAAGCTTAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTgg                                                                                                            <  1:422880/274‑1 (MQ=255)

                                                                                                                                                           agatGTCACCACAGATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTTCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCAGCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTgg                                                                                                            <  2:394602/272‑1 (MQ=255)

                                                                                                                                                               gCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTCTTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTa                                                                                                                >  1:76031/1‑266 (MQ=255)

                                                                                                                                                                 caccaTATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGtgatga                                                                                                     <  2:79803/275‑1 (MQ=255)

                                                                                                                                                                    caTATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATgaagg                                                                                                  <  1:148196/275‑1 (MQ=255)

                                                                                                                                                                         atTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTg                                                                                             <  1:73976/275‑1 (MQ=255)

                                                                                                                                                                             cGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCa                                                                                         >  2:29163/1‑275 (MQ=255)

                                                                                                                                                                              gTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCa                                                                                         >  2:427271/1‑274 (MQ=255)

                                                                                                                                                                                cgaaaggttgataagagacagaGTTCCGATAGAGCATACGTCCTGTTTTTGTAATTCCATCGGATTCTCTTCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCAt                                                                                        <  1:218928/252‑1 (MQ=255)

                                                                                                                                                                                cTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCt                                                                                      <  2:191141/275‑1 (MQ=255)

                                                                                                                                                                                         aGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTaataat                                                                              >  2:312435/1‑274 (MQ=255)

                                                                                                                                                                                         aGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAAtt                                                                             >  2:60248/1‑275 (MQ=255)

                                                                                                                                                                                           gTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAAtt                                                                                                                                                                                                            >  2:331764/1‑146 (MQ=255)

                                                                                                                                                                                             gATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCaa                                                                                                                                                                                                                                                                    <  1:454461/88‑1 (MQ=255)

                                                                                                                                                                                             gATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCa                                                                                                                                                                                               <  1:199167/157‑1 (MQ=255)

                                                                                                                                                                                             gATTTTCAAGTTCCGATAGAGCATACGTACTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGa                                                                          <  1:312435/274‑1 (MQ=255)

                                                                                                                                                                                               ttttCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATgg                                                                                                                                                                                                        <  1:331765/146‑1 (MQ=255)

                                                                                                                                                                                                     atttCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATTATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGaa                                                                   <  1:363048/271‑1 (MQ=255)

                                                                                                                                                                                                         ccGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCa                                                              <  2:95980/274‑1 (MQ=255)

                                                                                                                                                                                                             tAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCt                                                         <  1:131525/275‑1 (MQ=255)

                                                                                                                                                                                                               tggCATACTTCCTGTTGTTTTAATACCAGCGGATTTTCTTCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTACGGGTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTa                                                       <  2:260614/273‑1 (MQ=255)

                                                                                                                                                                                                                 gCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAggctgtctcttat                                                      >  1:414189/1‑263 (MQ=255)

                                                                                                                                                                                                                      cGTCCTGTTGTTGTAATACCATCGTATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCAt                                                <  2:281934/275‑1 (MQ=255)

                                                                                                                                                                                                                      cGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCAt                                                <  2:154958/275‑1 (MQ=255)

                                                                                                                                                                                                                       gTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                    >  1:155548/1‑270 (MQ=255)

                                                                                                                                                                                                                                  gtAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGt                                    <  1:40709/275‑1 (MQ=255)

                                                                                                                                                                                                                                   tAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                    >  1:50335/1‑258 (MQ=255)

                                                                                                                                                                                                                                    aaTACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTa                                  <  1:60248/275‑1 (MQ=255)

                                                                                                                                                                                                                                     aTACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGGCAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGAGTAAACTCACTTTCGACTATTGGGTGATGAAGTCAGTGAGCATCTGTAATAATTTGACTATGAAGGGCATTGCTTAATGGAATAGACATAATAGttcc                                 >  2:345013/1‑273 (MQ=255)

                                                                                                                                                                                                                                     aTACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGa                                                                    >  1:109452/1‑240 (MQ=255)

                                                                                                                                                                                                                                       aCCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCAt                                                <  2:50335/258‑1 (MQ=255)

                                                                                                                                                                                                                                         cATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAggg                                                                <  2:109452/240‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGActc                                                                       >  2:323889/1‑232 (MQ=255)

                                                                                                                                                                                                                                             ggATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTaa                                                      >  1:49092/1‑246 (MQ=255)

                                                                                                                                                                                                                                                 tctcTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTa                     <  1:358184/275‑1 (MQ=255)

                                                                                                                                                                                                                                                      gCATGCTACTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCTGAAGATATATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCa                <  1:384845/275‑1 (MQ=255)

                                                                                                                                                                                                                                                       cATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTg                                                                     <  2:62258/221‑1 (MQ=255)

                                                                                                                                                                                                                                                                gTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGAAAACTCACTGTCGACTATTGGGtga                                                                                                        <  2:335480/177‑1 (MQ=255)

                                                                                                                                                                                                                                                                    caTAATTAAATGGCCAAGACTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATTGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGaaa  <  1:176649/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                     aTAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTAt                          <  1:9893/250‑1 (MQ=255)

TCCTATCGTGGTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTCTTTACTAATATCAAGTTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAA  >  NC_000913/4555827‑4556361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`