New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4584202 =28 (0.670)3 (0.070) 3/272 6.2 9.4% coding (2561/3513 nt) hsdR endonuclease R Type I restriction enzyme
?NC_000913 4584216 = 31 (0.770)coding (2547/3513 nt) hsdR endonuclease R Type I restriction enzyme
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGA  >  NC_000913/4584095‑4584340
                                                                                                           |                                                                                                                                          
tGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACCCGCGCCACCGCGCTGTAGATATCGACACAGTCa                                                                                                         >  1:514173/1‑143 (MQ=255)
     ccATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTCCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAAt                                                                                                    <  2:556131/143‑1 (MQ=255)
      cagacGCTTCGGTGATTCTATAGGTTGCTGAATCGGTAATTTCAGTGACCAGCGTGTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATc                                                                                                   <  2:53397/139‑1 (MQ=255)
       aTCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTTCAGTTCCACCTTCGGGCGCACCACCACCGGGCGCATGGTGTCGACGCTCTCCAGCGTGCTGTGGATATCGACACAGTCAAACATCt                                                                                                  >  2:17083/1‑143 (MQ=255)
       aTCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGATGTCGCCGCTCTCCATCGTGCTGTTGATAGAGTACCAGTCAAAAATcg                                                                                                  >  1:164064/1‑142 (MQ=255)
        tCCGCTTCAGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCtt                                                                                                 <  2:42548/143‑1 (MQ=255)
               cGGTGATTTTATAGGTTTCTGAACCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTg                                                                                          <  2:131265/143‑1 (MQ=255)
                    aTTTTATCGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGtttt                                                                                     <  2:523147/143‑1 (MQ=255)
                       ttATAGGTGTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTAtt                                                                                  <  1:96726/143‑1 (MQ=255)
                             gTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGCCGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTCAAGCTGGTTTTCTTCGCCTc                                                                            >  2:364473/1‑143 (MQ=255)
                             gTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCCTGGTGTCGCCGCCAATCTGCGGGCTGTCGAGCTCGACGCAGTCAAAACGCCGCAAGCTGGttgttagcagcgc                                                                            >  1:255246/1‑132 (MQ=255)
                               ttCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACCGTCAAACATCTTAAAGCTGGTTTTATTCACCTCCg                                                                          >  2:489478/1‑143 (MQ=255)
                                         gTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGGCGCATGGTGTCCACGCTCTCCAGCGTGCGGTGGATACCGACACAGTCACGCCTCTTAAACCTGGTGTCAACCACACCCGGGCATacccc                                                                >  1:56451/1‑139 (MQ=255)
                                         gTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAcgcg                                                                >  1:679248/1‑143 (MQ=255)
                                                 aTTGACCAGCGTTTGCATCTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGgcgc                                                        <  2:46732/143‑1 (MQ=255)
                                                  ttGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCCCCGGACGCATGGTGTCGACGCTCTCCAGCGGGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCCTACGCGCGTGGcgcg                                                       >  2:78970/1‑143 (MQ=255)
                                                  ttGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATGAGCGCGTGGcgcg                                                       >  2:388235/1‑143 (MQ=255)
                                                         gCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAACTCTTAAAGCTGGTTTTCTGCACCGCCGTGCATCAGCGCGTGGCGCGGCCTTTc                                                >  2:304011/1‑143 (MQ=255)
                                                           gTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCAt                                              <  2:423910/143‑1 (MQ=255)
                                                                 aGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCGAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTc                                        <  2:594964/143‑1 (MQ=255)
                                                                 aGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTTTCCGCTGGGGATAGGGGCGAGGCGCGGCCTTTCTTCTGTTc                                        >  2:455444/1‑143 (MQ=255)
                                                                 aGTTCCACCTGCGGGCGCGCCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTc                                        <  2:499839/143‑1 (MQ=255)
                                                                   ttCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGCCGCTCTCCAGCGTGCTGTAGATATCGCCAGAGGCAAAAACCTCGACGCCGGTTTGATTCACCTCCGCGCATAAGGGGGTGGCGCGGCCtcccggcagtacgt                                      >  1:398393/1‑130 (MQ=255)
                                                                              ggCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCgg                           <  2:560808/143‑1 (MQ=255)
                                                                                  caccaccacCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGc                       <  2:630175/143‑1 (MQ=255)
                                                                                         accGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACttt                <  1:337919/143‑1 (MQ=255)
                                                                                            ggACGCAGGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACg             <  2:277443/143‑1 (MQ=255)
                                                                                                       gccGACGCGCGCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTGTTATTCACCTCCGGGCCTAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGAGTACTTGACGCAGGAACACGa  <  1:262001/141‑1 (MQ=255)
                                                                                                           |                                                                                                                                          
TGCGGCCATCCGCTTCGGTGATTTTATAGGTTTCTGAATCGGTAATTTCATTGACCAGCGTTTGCAGTTCCACCTTCGGGCGCACCACCACCGGACGCATGGTGTCGACGCTCTCCAGCGTGCTGTAGATATCGACACAGTCAAAAATCTTAAAGCTGGTTTTATTCACCTCCGGGCATAAGCGCGTGGCGCGGCCTTTCATCTGTTCGTACAGAATGCGGCTGCGTACTTTACGCAGGAACACGA  >  NC_000913/4584095‑4584340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.