Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,004,531 G→T 5.0% Q56K (CAA→AAA)  yigI ← 4HBT thioesterase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,5310GT 5.0% 758.1 / 2.0 250Q56K (CAA→AAA) yigI4HBT thioesterase family protein
Reads supporting (aligned to +/- strand):  ref base G (81/146);  new base T (18/2);  total (102/148)
Fisher's exact test for biased strand distribution p-value = 2.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 254 total aligned reads displayed.

GCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTA  >  NC_000913/4004260‑4004802
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                 
 aaaatttaaGTTGTCCTTCATTGGTGAAGTCGACGCGGGCGCCGGCGACTGTATTGCCGGCACGCAAAAGGCTACTAGTAGCAGTAAAACGCTCCCCCCTGCCTGGTCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGcc                                                                                                                                                                                                                                                                               <  2:498051/268‑1 (MQ=255)
          gCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGGTACTT‑CCCACGCAAACCAGACCGGCGGCGCCATCCCGCGCC‑GACCCACAG‑ACCCCGCC‑CTGCAAAAA‑CCTTTgtcaccagtgtcc                                                                                                                                                                                                                                                                       >  2:368505/1‑262 (MQ=255)
          agagacggtcATTGTGGAATTCAAGGCGGAGGAAGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGGCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGcc                                                                                                                                                                                                                                                                      <  2:838632/266‑1 (MQ=255)
              ttcttcATTGTGTAATTCAACGCGGGCGACGGCGTCTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCCATAGCCGCCGGCGTAGTTCATCTTCACTGATGGTTCCGTGGCGGGGGTAGGTTCTTCCCCACGC‑CACCCAACAGGCCGCGCAAACCAGCGCC‑GACGCAATGAACCCCCCC‑GTCCAAAGT‑CCTTGCCCCCCA‑GTT‑Cccacca                                                                                                                                                                                                                                                                 >  2:1072589/1‑275 (MQ=255)
              ttcttcATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTACGGGACTT‑CCCACCCCCACCCCACCGGCGGCGCGCACCCGCGCCGGACAC‑CTG‑ACCCCCCC‑GGTCAAAAT‑ACTTTTCCGCCC‑GTTGCccccca                                                                                                                                                                                                                                                                 >  2:791057/1‑275 (MQ=255)
              ttcttcATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GCCGCAATG‑ACCCCCCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTTCccacca                                                                                                                                                                                                                                                                 >  1:853614/1‑275 (MQ=255)
                     ttGTGTAATTCAACGCGGGCGACGGTGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTgg                                                                                                                                                                                                                                                          <  1:691610/275‑1 (MQ=255)
                      tgtgTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACCCCACACCCGCGGCGACACCCCGCCCC‑GACACCATG‑ACCCCGCCGGTCCAAAAT‑‑CTTTGCGCCCA‑GTTTCCCACCATCATTgg                                                                                                                                                                                                                                                          >  2:31141/1‑274 (MQ=255)
                      tgtgTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGGTACTT‑CCCCCGCACACACGACCGGCGGCGACACCCCCGGCC‑GACGGAAAG‑ACCCCCCC‑CCGCAAAAA‑GCTTTTCGCCCA‑GTTGTCCCCCCTCCTTgg                                                                                                                                                                                                                                                          >  2:1462767/1‑274 (MQ=255)
                        tgtAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTGAGGTACTT‑TCCACGCACACCCGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑AACCCGAC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTg                                                                                                                                                                                                                                                       >  1:1071516/1‑275 (MQ=255)
                        tgtAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCCGACCGGCGGCGACCTCCAGCCCC‑GACGCCATG‑ACCCCCCC‑GTGCAAAAT‑GCCTGGCGCCCA‑GTTGCCCACCATCATTTGCTg                                                                                                                                                                                                                                                       >  1:502171/1‑275 (MQ=255)
                        tgtAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCCCCGGCGACCTCCCGCGCC‑GACGCAATG‑ACCCCCCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCCTTGGCt                                                                                                                                                                                                                                                        >  1:544367/1‑274 (MQ=255)
                        tgtAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GCAGAAATG‑GATCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑TTTGCCCACCCTAATTTGCtt                                                                                                                                                                                                                                                       >  1:1171518/1‑274 (MQ=255)
                        tgtAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGGTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGCGACTG‑CCCACGCCCAACAAACCGGGGGCGCACTCCAGGCCC‑GACCCCAAG‑ACACCCCC‑CGGCCACAT‑GCCGTGCGCCCACGTTGccccccctactgtgca                                                                                                                                                                                                                                                        >  2:1506256/1‑265 (MQ=255)
                          tAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGGACAT‑CCCCCGCACACCAGAACCGCGGCGACCTCCCGCGCC‑GACGCACTG‑ACCCCGCC‑GTGCAAAATCGCTTT‑CACCCA‑CTTGTCCCCCCCTATTGGCTgt                                                                                                                                                                                                                                                      >  2:1080431/1‑273 (MQ=255)
                               ctccgcgGGAGGCGGCGACTATATTGCCGGAACGCAACAGGCTACTATCGTGAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCGGGCGTAGTTCATCTTCACTGATGGGTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTa                                                                                                                                                                                                                                                <  2:389145/272‑1 (MQ=255)
                                  cgcgGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCCGATTTTTaaa                                                                                                                                                                                                                                              >  1:79581/1‑274 (MQ=255)
                                  cgcgGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATATTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCCCACCACACCGGCGGCGCCCTCCAGCGCC‑CACGAGATG‑ACCCCCGC‑CTTCAAAAA‑GGTTTGCACCGC‑CTTGCCCACCCACCTTCGCTGATTTTTAAAg                                                                                                                                                                                                                                             >  2:201011/1‑275 (MQ=255)
                                       gcgacggcgacTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGCGTAAGGTACTT‑CCTACGCCCCCCAGAACGGCGGCGACATCCAGCGAC‑GCCGCAATG‑AACCCGCC‑GTGCAAAAA‑GCTTTTCGCCCA‑GTTGCCCACCACCATTTGCTGATTTTTTAAGGCTAg                                                                                                                                                                                                                                        >  2:1488092/1‑275 (MQ=255)
                                        cgacggcgacTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGc                                                                                                                                                                                                                                       <  1:1080432/275‑1 (MQ=255)
                                                acTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGaa                                                                                                                                                                                                                               <  1:1462768/275‑1 (MQ=255)
                                                           tGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGGTACTT‑CCCACCCACACCAGACCGGGGGCGGCATCCCGGCCC‑GCAGCACTG‑ACCCCGCC‑GCGGAAAAT‑GCTTTTCGCCCA‑GTTGCCCACCACCATTTGCTGTTTTTTTAAGGCAAgtcttgccacaccttttctg                                                                                                                                                                                                                     >  2:111621/1‑255 (MQ=255)
                                                           tGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGGGACTT‑CCCCCGCACACCCCACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑AACCCCCC‑CGGCAAAAA‑GCTTTGCGCCCC‑GTTTCCCACCCCCATTGGCCGATTTTTAAATGCCAGCCGGGTGAACCCTTTTTcc                                                                                                                                                                                                                     >  2:511778/1‑273 (MQ=255)
                                                                        ctactaGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGtt                                                                                                                                                                                                        <  1:198365/274‑1 (MQ=255)
                                                                          actaGTAACAGTAAAACGCCCGCCCCTGCCTGGGGGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGGGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAAGG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCa                                                                                                                                                                                                     <  2:1315119/275‑1 (MQ=255)
                                                                           ctaGTAACAGTAAACACCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGGTAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTG‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTGTTCGTAACGCTCCAGTTCCa                                                                                                                                                                                                     <  2:120673/274‑1 (MQ=255)
                                                                                      aaaaCGCCCCCCCCTGCGTGTGCGCAGATAATCAACGCGAAGATAAATGGTCCCCATCCGCGATAGCCGCTTGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATgcg                                                                                                                                                                                          <  2:966692/274‑1 (MQ=255)
                                                                                      aaaaCCCCCCCCCCTGCCTTGGGCCAAATAATCAACCCGAAGTTCAATGGTCCCCATCCGCGGTTGCCGCTGGGGTAGTTCATCTTCACTGATGGTTTCGTGTCGGGTTAAGGTACTT‑CCCACGGACACCAGACCGGCGGCGACAGCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTTGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATgcg                                                                                                                                                                                          <  2:455294/274‑1 (MQ=255)
                                                                                         aCGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGt                                                                                                                                                                                                         <  1:516052/256‑1 (MQ=255)
                                                                                              cGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTGAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTaaa                                                                                                                                                                                  <  1:31141/274‑1 (MQ=255)
                                                                                                    tgcctgGGCGCATATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGc                                                                                                                                                                              <  1:274682/272‑1 (MQ=255)
                                                                                                    tgcctgGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGc                                                                                                                                                                              <  1:111622/272‑1 (MQ=255)
                                                                                                      cctgGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTTAGGGACTT‑CCCCCGCCCACCAGACCGGCGGCGGCATCAACCCCC‑CACCCCATG‑ACCCCCCC‑GCGAACAAA‑GCTTTGCGCCCA‑GTTTCCCACCATCCTCTGCTGACTTTTAAAGGCCCGCTGTCCGAACTCTTTTTCGTTACCCTCCAATTCCACCCCCAAACCCCGGCTAAAAGCCAttt                                                                                                                                                                          >  2:1243092/1‑272 (MQ=255)
                                                                                                                gATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCCACCTCCCGCGCC‑GACGCCATG‑ACCCCCCC‑GTGCAAAAA‑GCTTTTCGCCCC‑GTTTCCCCCCCCCCTTGGCCGAtttttt                                                                                                                                                                                                                                                >  2:1104510/1‑193 (MQ=255)
                                                                                                                         cgcgAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCAc                                                                                                                                                       >  2:644334/1‑273 (MQ=255)
                                                                                                                         cgcgAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCAc                                                                                                                                                       <  1:644335/273‑1 (MQ=255)
                                                                                                                             aaGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACt                                                                                                                                                  <  1:1033677/274‑1 (MQ=255)
                                                                                                                                       ggccccATCCGCGATAGCCGCGGGCGTAGTTCGTCTTGACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACt                                                                                                                                                  <  1:1308955/262‑1 (MQ=255)
                                                                                                                                       gTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGc                                                                                                                                       <  1:1181683/275‑1 (MQ=255)
                                                                                                                                       gTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGc                                                                                                                                       <  1:382273/275‑1 (MQ=255)
                                                                                                                                          cccATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCTGGTTAAAGTACTT‑CCCACGCACAACAGACCAGCGGCGACATCCAGCACC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTTCGCCCA‑GTTGCCCACCATCATTGTCTGATTTTTAAAGGCCAGCTGTTCGAACTCTTTTTCGTAACACTCCAGTTCTATCCCCAAAGCGTGGTTAAATGGCACAGTAT‑AAACAAAACTCTCACCCACTAACTTCAGGGCtt                                                                                                                                     >  1:951447/1‑274 (MQ=255)
                                                                                                                                          cccATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCCCGCCCACCACACCGGCGGCGACATCCACCGCC‑GACGCAACC‑ACCCCGCC‑GTTTCAAAT‑GCTTTGCCCCCA‑GTTCCCCACCATTCTTTGCTGATTTTTCTAGGCCCGATGTGCGAACACCTTTTCGTAACGCTCCAGTTCCACACCCAATGCGCGGGTAAATGGCATGTGTTAAAACAAACA‑CTCCACCACTTAATTCAGGGGTTg                                                                                                                                    >  2:1258308/1‑275 (MQ=255)
                                                                                                                                              tCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGc                                                                                                                                                                                                                                           <  1:496656/169‑1 (MQ=255)
                                                                                                                                              tCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTaa                                                                                                                                                                                                                  <  1:982741/194‑1 (MQ=255)
                                                                                                                                                  cgATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACCCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGGGAAATCTTTTTCGTAACGCCCCCGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCCACCCCCCTAATTTCAGGGCTTGTTCAg                                                                                                                               >  1:1025000/1‑272 (MQ=255)
                                                                                                                                                   gtgaGCCGCTGGCGTCGTTCATCTTCACTGATGGTGTCGTGGCGGGTTAAGGTACTT‑CCCACGCTAACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATT‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGt                                                                                                                           <  2:1528221/272‑1 (MQ=255)
                                                                                                                                                   gATAGCCGCTGGCGGAGTTCATTCTCCCTGATGGTTTCGTGGCGGGTTAAGGTACTC‑CCCACGCACACCAGACCGGCGGCGACAGCCAGCGCC‑GACCCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGt                                                                                                                           <  2:1024999/275‑1 (MQ=255)
                                                                                                                                                     tggCCGCTGGCGTAGTTCAGCGTCCCGGGTGGTTTCTTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCTACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTTCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCa                                                                                                                                <  2:1032925/266‑1 (MQ=255)
                                                                                                                                                        ncNCTGGCGTAGTTCATCTTCACCTAGGGTGTCGTGGACGGGTAAGGGACGT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCTCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTa                                                                                                                      <  2:1171517/274‑1 (MQ=255)
                                                                                                                                                             tgCGTAGTTCAGCTTCACTGATGGTTTCGTGGCGGGTTAAGGAACTT‑CCCACGCACACCAGAACGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCa                                                                                                                 <  1:1474733/274‑1 (MQ=255)
                                                                                                                                                                    ttCAACCTCACTGATGGGTTCGTTGCGGGTTCAGGGACTT‑CCCACCCACCCCAGACCGGCGGCGACAGCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCCGGGCTTGTTCAGCGGTCAGTACGGCAGACatat                                                                                                          <  2:694039/275‑1 (MQ=255)
                                                                                                                                                                     tcctCTTCACGTATGGTTTCGTTGCGGTTTCAGGTACTT‑CCCACGCCCGCCAGACCGGCTGCGACATCCAGCGCC‑GGCGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat                                                                                                          <  2:916777/271‑1 (MQ=255)
                                                                                                                                                                     tCATCTTGACTGCGGGTTTCGTTGCGTGTTAAGGTAGTT‑CCCACGCAAACGAGACCGGCCGCGACATCCAGCGCC‑GCCGCAATG‑ACCCCGTC‑GTGCAACAT‑CCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAAATCTTATTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATGTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat                                                                                                          <  2:932233/274‑1 (MQ=255)
                                                                                                                                                                     tCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCGCGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAGACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat                                                                                                          <  1:1497381/274‑1 (MQ=255)
                                                                                                                                                                     cgcgcttccaggATGGTTTCTTGGCGGGGTAAGGTGCTT‑CCCACGCAACCCTCGCCGCGGGCGTCCTCCAGGGCC‑GACGCCATG‑ACCCCGCC‑GTGCAAAAT‑GCGTTGCGACCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGAATGTGAT‑AAACAAACATCTCACCCACTAATTTCATGGCTTGTTCAGCGGTCAGTACGGGAGACatat                                                                                                          <  2:544366/263‑1 (MQ=255)
                                                                                                                                                                       accTTCACTGACGGTTGCGTGGCGGGCTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCCGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatc                                                                                                         <  2:875704/271‑1 (MQ=255)
                                                                                                                                                                       aTCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatcat                                                                                                       <  1:332114/275‑1 (MQ=255)
                                                                                                                                                                           tccttgaggcgttCGTTGCTGATCAAGGTACTT‑CACAGTATCACCAGACCGGCGGCGAGTTCCAGCGCC‑AACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGGCCA‑GTTCCCCACCTTCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAAAGCTCCAGTTCCTTCCCCAATGCGCGGTTAAGTGGCATGTAAT‑ACACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCt                                                                                                    <  2:299575/263‑1 (MQ=255)
                                                                                                                                                                            cACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGc                                                                                                                                                                              <  1:1192427/200‑1 (MQ=255)
                                                                                                                                                                              cTGATGTTTTCGTTGCGGGTTAAGGTACTT‑CCCACGCACACTAGACCGGCGGCGACCTCCAGCGCC‑TACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTCGCTGATTTTTAAAGGCGCGTTGGGCGAATTTTTTTTCGTACCGGTCCAGTTCCCTCTCCAATTCGCGGTTAAATGGCTTGTGCT‑AACCACAGCGCTCAACCACTAATTGCCGAGCTTGTTCAGCGGTCAGTCCGGCAGATATATCATCCTTaca                                                                                                <  2:1019219/275‑1 (MQ=255)
                                                                                                                                                                                   tgTTTCGTGGCGGGTTAATGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCTATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTAGCTGATTTTTAAATGCCCGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCa                                                                                           <  1:1258309/274‑1 (MQ=255)
                                                                                                                                                                                                   aaGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATgcg                                                                                                                                                                                          <  1:1398634/165‑1 (MQ=255)
                                                                                                                                                                                                         cTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCaa                                                                                                                                                                                              >  2:694502/1‑155 (MQ=255)
                                                                                                                                                                                                           t‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTa                                                                   >  1:510874/1‑275 (MQ=255)
                                                                                                                                                                                                              ccACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATgcg                                                                                                                                                                                          <  1:694503/155‑1 (MQ=255)
                                                                                                                                                                                                               cACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCTCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATg                                                                 <  1:1236604/274‑1 (MQ=255)
                                                                                                                                                                                                                 cGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAAT‑‑GCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGctt                                                              >  1:573383/1‑273 (MQ=255)
                                                                                                                                                                                                                    acacCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGAc                                                                                                              >  1:1522625/1‑224 (MQ=255)
                                                                                                                                                                                                                     cacCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTCAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTACATCCCCAATGCGCGGTTAAATGGCATGTGCT‑AAACCAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCATTACGGCAGACATATCCTCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGcttctt                                                           >  1:1096879/1‑274 (MQ=255)
                                                                                                                                                                                                                     cacCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTc                                                                                                                                                                                                             <  1:486492/129‑1 (MQ=255)
                                                                                                                                                                                                                      acCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGcttctt                                                           >  1:605096/1‑273 (MQ=255)
                                                                                                                                                                                                                        cAGACCGGCGGCGACATCCAGCGCC‑GCCGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat                                                                                                          <  2:1522624/224‑1 (MQ=255)
                                                                                                                                                                                                                        cAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTTCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAATGCCAGCTGTGCGAACTCTTTTTCGTAACGCGCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATCTGAT‑ACACAAACATCTGACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGAAATATCATCCTTACACTTCATTTGTTAATGAAATGTTGATTTTATACTTTTttgtt                                                       >  1:674767/1‑275 (MQ=255)
                                                                                                                                                                                                                        cAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCa                                                                                           <  1:921877/239‑1 (MQ=255)
                                                                                                                                                                                                                         aGACCGGCTGCGACATCCAGCGCC‑GACGCAATG‑ACCCCTCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTTGCTGATTTTTAAAGGCCATCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTttgttg                                                      <  1:1002219/275‑1 (MQ=255)
                                                                                                                                                                                                                            ccGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCCCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTAAGGGCTTGATCAGCGGTCAGTACGGCAGACATATCAACCTTACACTTACTTGGTTCATGAAATGTTGATTTTATGCATCTgtgttgttg                                                   >  2:535400/1‑275 (MQ=255)
                                                                                                                                                                                                                                cggccACATTCAGCGCC‑GACGCAATG‑ACCCCTCC‑GTGTCAAAG‑ACTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTGTTAAAGGCCAGCCTTGCGAACTCTTGTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGttt                                               <  1:802820/275‑1 (MQ=255)
                                                                                                                                                                                                                                          ccgcgcC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCTCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAgg                                     <  1:1571116/273‑1 (MQ=255)
                                                                                                                                                                                                                                           tgcgcC‑TGCGAAATG‑ACCCCGCC‑ATGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATTCGCGGTTAAATGGCATGTCAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGa                                    <  2:184282/274‑1 (MQ=255)
                                                                                                                                                                                                                                           aGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGa                                    <  1:405788/275‑1 (MQ=255)
                                                                                                                                                                                                                                           aGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGa                                    <  1:1126731/275‑1 (MQ=255)
                                                                                                                                                                                                                                                  gACGCAATG‑GCCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCTTCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGa                               <  1:1336474/274‑1 (MQ=255)
                                                                                                                                                                                                                                                   aCGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTttgttgttg                                                   >  2:745214/1‑253 (MQ=255)
                                                                                                                                                                                                                                                    cGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATatcatc                                                                                                      >  1:1159001/1‑201 (MQ=255)
                                                                                                                                                                                                                                                    cGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGAtaa                            >  2:1353695/1‑275 (MQ=255)
                                                                                                                                                                                                                                                      cAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGAtaact                          <  2:471678/275‑1 (MQ=255)
                                                                                                                                                                                                                                                      cAAGG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑CTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGAtaact                          <  1:1421088/275‑1 (MQ=255)
                                                                                                                                                                                                                                                       aaTG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGttt                                               <  1:745215/253‑1 (MQ=255)
                                                                                                                                                                                                                                                        aTG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTa                                                                                                  <  2:1159000/201‑1 (MQ=255)
                                                                                                                                                                                                                                                        aTG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGAtaactaa                        <  1:1524006/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                cgcc‑gTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCCGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACACCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATACTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTAt                 >  1:762163/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                cgcc‑gTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTa                  <  1:1353696/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                cgcc‑gTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTAt                 <  1:259492/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cc‑gTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGAtaacta                         >  2:588446/1‑265 (MQ=255)
                                                                                                                                                                                                                                                                     ttccAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCCTTGGCTGAGTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAAT‑‑GCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGtt           <  2:573382/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACatat                                                                                                          <  2:17454/180‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACgg                     <  1:588447/265‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aacaT‑GCTTTGGGCCCA‑GTTTCCATCCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTaa         <  2:811054/272‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aattgtttGCGCCCA‑GTGGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTTAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATGGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGt       <  2:951446/270‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tttGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACCAATTTCCGGGCTTGTTCCGCGGCCAGTACGGCAGACATATCAATCTTTCACTTCATTGGTTAATGAAATGTTGCTTTTATGCTTCTTTGTTGTTGGTTTTTCCTTTCGGCAGGGATATCTAACGGCTATGGCTTTAAGtgtgca  >  2:232750/1‑273 (MQ=255)
                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                 
GCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTT‑CCCACGCACACCAGACCGGCGGCGACATCCAGCGCC‑GACGCAATG‑ACCCCGCC‑GTGCAAAAT‑GCTTTGCGCCCA‑GTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGTAACGCTCCAGTTCCATCCCCAATGCGCGGTTAAATGGCATGTGAT‑AAACAAACATCTCACCCACTAATTTCAGGGCTTGTTCAGCGGTCAGTACGGCAGACATATCATCCTTACACTTCATTGGTTAATGAAATGTTGATTTTATGCTTCTTTGTTGTTGGTTTCTACTTTAGGAAGGGATAACTAACGGCTATGGAGTTAAGTATGTA  >  NC_000913/4004260‑4004802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑