Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA97.9% 131.0 / NA 49P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (26/21);  minor base G (0/1);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184405‑4184679
                                                                                                                                         |                                                                                                                                         
tgcgtgtacaCGCTGTATCCAGGCTGGTGACAAGATGGCAGGTCGTCACGGTAGCAAGTGCGGAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                      <  1:87600/134‑1 (MQ=255)
ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                      <  2:373944/143‑1 (MQ=255)
ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGGCGTCACGGTAAAAAGGGTGTAATTTCTAAGATCAACACGATCGAAGGTAAGGCTGAAGATGAAGACGGGACGACGGGAGACATCGTACTGAACGAGCTgg                                                                                                                                      >  1:254687/1‑143 (MQ=255)
     ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                 <  2:606638/143‑1 (MQ=255)
       aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGCAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                               >  1:224880/1‑143 (MQ=255)
       aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                               <  2:415745/143‑1 (MQ=255)
            ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGtcacgccgc                                                                                                                          >  1:722447/1‑135 (MQ=255)
               tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGAACCGTCTCGTa                                                                                                                       >  1:209198/1‑143 (MQ=255)
                aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGCACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                      >  1:2137/1‑143 (MQ=255)
                   cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                   <  1:106404/143‑1 (MQ=255)
                        tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGCAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                              <  2:27043/143‑1 (MQ=255)
                          gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAtcaccctccgt                                                                                                            >  1:723281/1‑133 (MQ=255)
                           tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGCTTAAACCGGGCGAAGATGTGAATGAGAAAGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCgggc                                                                                                           >  2:352270/1‑141 (MQ=255)
                                   tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTGTAAGCTCAACGCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                   <  1:544161/143‑1 (MQ=255)
                                    ggCAGGTCGTCACTGTAACAAGGTTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCg                                                                                                  <  2:163570/143‑1 (MQ=255)
                                    ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACTAGTTGGGAGGGCCACCACCTATGCACATCGGTCAGATCCTca                                                                                                  >  2:138600/1‑142 (MQ=255)
                                    ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACACCGGTCAGCTCCTCg                                                                                                  >  2:651024/1‑143 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                               <  2:205805/143‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGGAAGCCGGTAGACAGCGTACTGAACCAGCTGGGCGAACCGTCGCGCAATAACCTCGGCCCAATCCTGGaaa                                                                                               >  1:84921/1‑143 (MQ=255)
                                                cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                      >  2:82194/1‑143 (MQ=255)
                                                   gaacggtggTGGAATTTCTAAGAGCGACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTCGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                   <  1:637157/136‑1 (MQ=255)
                                                         gggTGTAAGTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                             <  2:11956/143‑1 (MQ=255)
                                                          ggTGTAATTTCTAAGATCAACCCTATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCATATCCTCGATACCCACCTGGGTATGGCTGc                                                                            <  1:100962/143‑1 (MQ=255)
                                                              tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGCTCCCGGGAACACAACCGGGGAAGGCTGCGaaa                                                                        >  1:153787/1‑143 (MQ=255)
                                                              tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACAGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAAATCGGTAAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                        >  2:329263/1‑143 (MQ=255)
                                                               aaTTTCTAAGGTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                       <  1:713493/143‑1 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                 >  2:374839/1‑143 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTGTCg                                                                 >  1:409902/1‑143 (MQ=255)
                                                                         aTCAACCCGATCGAAGAGAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                             <  2:98477/143‑1 (MQ=255)
                                                                            gaCCCGATTGAAGATATGCCTTTGGATGAAATCGGTACGTCGGTAGACATCGTACTGAACCAGCTGGGCTTACCGGCGCGTATGAACATCGGTCAGATCCTCGAAACCTACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                          <  1:618114/142‑1 (MQ=255)
                                                                             aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAACTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAGGGCTGCGAAAGGTAtcgtcggcgcg                                                         >  2:125429/1‑138 (MQ=255)
                                                                              cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGa                                                        >  2:382643/1‑143 (MQ=255)
                                                                               ccGGTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGAt                                                       >  1:133028/1‑143 (MQ=255)
                                                                                cGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTc                                                      >  2:421482/1‑143 (MQ=255)
                                                                                      aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACATGATCAACGcc                                                >  1:200661/1‑143 (MQ=255)
                                                                                         acatGCCCTACGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                             <  2:694154/141‑1 (MQ=255)
                                                                                                    gATGAAGGCGGTACGCGGGTAGGCATCGTACTGAACCTGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                  <  1:38153/143‑1 (MQ=255)
                                                                                                     aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                 >  2:619935/1‑143 (MQ=255)
                                                                                                        agcaCGGTACGCCGGTAGACATCGTACTGACCCATCTGGGCGAAACGCGACAATGGAGCATCGATCATAACCTCGACACCCACCCGGGTATGGCTGCGAACGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                              <  1:159843/140‑1 (MQ=255)
                                                                                                        aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTCTCGGCGACAAGATCCACGCCATGCTGAAACAGCAGCaa                              >  2:221186/1‑143 (MQ=255)
                                                                                                           aCGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  2:209198/143‑1 (MQ=255)
                                                                                                               tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                       <  1:648386/143‑1 (MQ=255)
                                                                                                               tACGCCGGCAGACATCGTACTGAACCAGCTGGCCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGGGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                       <  1:708591/143‑1 (MQ=255)
                                                                                                                    cGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAAAATCGGGCAGATCCTCGCAACCCACCTGGGCATGGCTGCGACAGGTCCCGGCGACACGCCCAACCCCCTGCACCCACAGCAGCACGAAGTcgacaca                  >  1:620749/1‑138 (MQ=255)
                                                                                                                         gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGCATGAACAGAGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAACAAGTCGCGAAACTgcg             >  1:344938/1‑143 (MQ=255)
                                                                                                                            aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGGAGTCGCGAAACTGCGCGa          >  2:321523/1‑143 (MQ=255)
                                                                                                                              cGTACTGAACCGGCTGGGCGTACCGGCTCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        <  2:329990/143‑1 (MQ=255)
                                                                                                                                  cTGAACCAGCTGGGCGTACCGTCACGTAGGAACAGCGGTCAGCTCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt    <  2:104166/143‑1 (MQ=255)
                                                                                                                                    gAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  >  2:43371/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4184405‑4184679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑