Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,370,460 (T)5→4 coding (320/639 nt) sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,370,4560T.97.8% 386.7 / 4.6 93coding (324/639 nt)sspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (41/47);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTC  >  CP009273/3370308‑3370600
                                                                                                                                                    |                                                                                                                                                
cGACCAGGCTGAACTCATCGCTCAGGACGTACGGCTTCTGACCGAAGCCCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCt                                                                                                                                                  <  2:198099/149‑1 (MQ=255)
      ggCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttcga                                                                                                                                            <  1:529298/149‑4 (MQ=255)
       gCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttcgat                                                                                                                                           >  1:1179119/1‑145 (MQ=255)
           aaCTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTcgatgcga                                                                                                                                      <  2:1477264/149‑1 (MQ=255)
            acccATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTcgatgcgat                                                                                                                                     <  1:1508119/146‑1 (MQ=255)
             cTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTcgatgcgatg                                                                                                                                    >  2:1309468/1‑149 (MQ=255)
                aTCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCAt                                                                                                                                 >  2:203625/1‑149 (MQ=255)
                aTCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGGCTTTTCGAGGCGATGCAt                                                                                                                                 >  1:1617541/1‑149 (MQ=255)
                aTCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGGGTACCAGTCTTTTCGATGCGATGCAt                                                                                                                                 >  2:110120/1‑149 (MQ=255)
                  cGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGACCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGt                                                                                                                               <  2:1352572/149‑1 (MQ=255)
                  cGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGt                                                                                                                               <  2:901810/149‑1 (MQ=255)
                   gCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTa                                                                                                                              <  2:586038/149‑1 (MQ=255)
                    cTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTAc                                                                                                                             >  2:1197495/1‑149 (MQ=255)
                       aGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGa                                                                                                                          >  2:444254/1‑149 (MQ=255)
                        agAAGTACGACTTCTGACCGAAGACCGGCGCAATCGCCAGAAGTTCTTCGCGCAGTGCCTTAAATGCGGCATCTGCTTAAGAGGCTGAACCGTTGATGATGGTGTTCATAAGCGTGTACCAGGAGTTTCGATGGAATGCATgcagcgac                                                                                                                         >  1:434313/2‑142 (MQ=255)
                            gTACGGTTTATGACCGAAGACCGGCGCCATCGCCAGCAGTTTTTCGCGCAGTTGCTTACGTGCGGTATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCt                                                                                                                     <  1:636479/149‑1 (MQ=255)
                            gTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCt                                                                                                                     <  2:812738/149‑1 (MQ=255)
                             tACGGCTTCTGACCGAAGACCGGCGCACTAGCCTGCATTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCtt                                                                                                                    >  2:1217298/1‑149 (MQ=255)
                              agggtctCTGACCGAGGCCCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGTACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCttt                                                                                                                   <  1:452319/143‑1 (MQ=255)
                               cGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATGTGCTTCCGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTTGACCAGGCTTTTCAATGCGAGGCTTGTACAGGCgacgtta                                                                                                                  >  2:372769/1‑143 (MQ=255)
                                ggCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCa                                                                                                                 <  1:795121/149‑1 (MQ=255)
                                  cTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTCCAGCCGGCTTTCAcc                                                                                                               >  1:634517/1‑149 (MQ=255)
                                    tCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACcgc                                                                                                             <  2:262841/149‑1 (MQ=255)
                                    tCTGACCGAAGACCGGCGCAACCGCCAGCATTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACcgc                                                                                                             >  2:859380/1‑149 (MQ=255)
                                     cTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACcgcg                                                                                                            >  1:959338/1‑149 (MQ=255)
                                      tgacggtggtccGGCGCAATCGGCGGGAGTTCTTCGCGCAGTTGCTTGCGTGCGGCATCTGCTTCAGAAGCTGGACCGTTGGTGGTGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGa                                                                                                           <  1:36757/139‑1 (MQ=255)
                                      tGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGa                                                                                                           <  1:1634452/149‑1 (MQ=255)
                                      tGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGa                                                                                                           <  2:500486/149‑1 (MQ=255)
                                      tGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGa                                                                                                           <  1:707009/149‑1 (MQ=255)
                                       gACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCGTGCAACGCGAg                                                                                                          >  2:355777/1‑149 (MQ=255)
                                           gAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTAc                                                                                                      >  2:468282/1‑149 (MQ=255)
                                           gAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTAGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCAAGTACAGACGGCTTTCACCGCGAGCTAc                                                                                                      >  2:1148905/1‑149 (MQ=255)
                                             aGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCg                                                                                                    <  1:1158334/149‑1 (MQ=255)
                                              gACCGGCGCAAGCGCCAGCAGTTCTTCGCGCATTTGCTTACGTGTGGCATCTGCTTCAGAAGCTGAACCGTTGATGATTGTTTTAATCAGGGGGTCCCAGTCTTTTCGATGCGATGCATGTACAGACCGCTTTCACCGCCAGCTACCgg                                                                                                   <  1:495125/149‑1 (MQ=255)
                                                 cGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGCGCTACCGGGTa                                                                                                >  2:90370/1‑149 (MQ=255)
                                                 cGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTGTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGCGCTACCGGGTa                                                                                                >  2:144196/1‑149 (MQ=255)
                                                     gcAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTCCCGGGTAAACa                                                                                            >  1:1122396/1‑149 (MQ=255)
                                                     gcAATCGCCAGCAGTTCCTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACa                                                                                            <  2:484092/149‑1 (MQ=255)
                                                           gCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGCGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCaac                                                                                      >  1:789526/1‑147 (MQ=255)
                                                                 agTTCTTCGCGCGGTTGCTTACGTGCGGCAGCTGCTTCAGAAGCTGACACGTTTAATATGGTGTTCATCAGCGGGCACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGc                                                                                <  2:314244/149‑1 (MQ=255)
                                                                      ttcGCGCAGTTGCTGCCGTGCGGCATCTGCTTACGCGGCTAAACCGTTGGTGATGGTGTTCATCAGCGTGTACCACTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATg                                                                           >  2:1215673/1‑149 (MQ=255)
                                                                       tagcgcAGTGGTTTACTTGCGCCATCTGCTGCACAAGCTGAACCGTTGACGATGGTGTTCATCAGCGTGGACCAGTCCTTTCGATGCGATGCAGGTACAGACGGCTTTCACCGCGTGCTACCGGGTAAACAGGCATCAGTGGCGGATGc                                                                          <  2:634572/147‑1 (MQ=255)
                                                                         gccgaGTTGCTTACGTGCGTCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCgg                                                                        <  1:880219/145‑1 (MQ=255)
                                                                         gcccAGTTGCTTACGTGCGGCATTGGCTGCAGGAGCTGAACCGTTGATGGTGGTGTTCATCAGCGTGTACCAGTCTTTTCGAGGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTTGCGGATGCgg                                                                        <  1:24028/146‑1 (MQ=255)
                                                                             aGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACGGACGGCTTTCACCGCGCGCTACCGGGTAAACAGGCATCAGTGGCGGATGAGGGaaa                                                                    >  2:407041/1‑149 (MQ=255)
                                                                             aGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGaaa                                                                    >  1:862579/1‑149 (MQ=255)
                                                                               ttGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTGCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGGAACg                                                                  >  1:271953/1‑149 (MQ=255)
                                                                               ttGCTTACGTGCGGCACCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTAATAAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCCAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACg                                                                  <  1:372769/149‑1 (MQ=255)
                                                                                 gCTTACGTGCGGCATCTGCTTCAGAAGCTGACCCGTTGAAGTTGGTGTTTATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCt                                                                <  2:1120205/149‑1 (MQ=255)
                                                                                 gCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCt                                                                >  2:97248/1‑149 (MQ=255)
                                                                                  cTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGCTGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTc                                                               <  1:1638854/149‑1 (MQ=255)
                                                                                  cTTACGTCCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTc                                                               <  2:144568/149‑1 (MQ=255)
                                                                                   ttACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATGAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCa                                                              >  2:1133477/1‑149 (MQ=255)
                                                                                   ttACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCa                                                              <  2:556497/149‑1 (MQ=255)
                                                                                   ttACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCCCa                                                              >  2:888283/1‑149 (MQ=255)
                                                                                   ttACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACAGCGAGCTACCGGGTAAACAGGCACCAGTGGCGGATGCGGGAAACGCtga                                                              >  2:42357/1‑147 (MQ=255)
                                                                                     aCGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGGAAACAGGCATCAGTGGCGGAGGCGGGGAACGctcctc                                                            >  1:1016904/1‑149 (MQ=255)
                                                                                       gtccGGCTTCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCa                                                          <  1:489329/146‑1 (MQ=255)
                                                                                       gTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCa                                                          <  2:772094/149‑1 (MQ=255)
                                                                                          cGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttcga                                                                                                                                            <  2:325168/65‑4 (MQ=255)
                                                                                          cGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCttttcga                                                                                                                                            >  1:325168/1‑62 (MQ=255)
                                                                                              aTCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTc                                                   <  2:530367/149‑1 (MQ=255)
                                                                                              aTCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTc                                                   <  2:1035149/149‑1 (MQ=255)
                                                                                               tCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTcc                                                  <  2:360158/149‑1 (MQ=255)
                                                                                                cTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATCTTCCa                                                 >  1:1549475/1‑149 (MQ=255)
                                                                                                 tGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGCCGGCTTGCACCGCGAGCTGCATTCTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCAt                                                >  2:315068/1‑149 (MQ=255)
                                                                                                 tGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCAt                                                <  2:251589/149‑1 (MQ=255)
                                                                                                 tGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCAATGCATTTACAGACGGCTTTAACCGCGAGCTACCGGGTAACCAGGCATCAGGGGCGGATGGGGGAAAGCCTCGTGCACATATTCCAt                                                >  1:516512/1‑149 (MQ=255)
                                                                                                 tGCTTCAGAAGCTGAACCGTTGATGATGGTGTCCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCAGCCAGATATTCCAt                                                <  2:1123065/149‑1 (MQ=255)
                                                                                                  gCTTCCGCAGCTGAACCGTTGATGAGGGTGCTCCTCAGCGTTTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTCCACCGTGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGACATTCCATa                                               <  1:914243/149‑1 (MQ=255)
                                                                                                   cTTCAGAAGCTGAACCGTTGATGATGGTGATCCTCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTAGCGGGTAAACAGGCATCAGTGGCGGGTGCGGGCAACGGTCATCCAGATATTCCATaa                                              >  2:161181/1‑149 (MQ=255)
                                                                                                   cTTCAGAAGCCGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGTGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATaa                                              <  2:1286817/149‑1 (MQ=255)
                                                                                                    ttCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGCCGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCACTGGCGGATGCGGGAACCGCTCATCCAGATATTACATatt                                             >  1:1297835/1‑147 (MQ=255)
                                                                                                    ttCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAat                                             <  2:549859/149‑1 (MQ=255)
                                                                                                    ttCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAat                                             <  1:859380/149‑1 (MQ=255)
                                                                                                       ataaGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAatgat                                          <  2:352105/147‑1 (MQ=255)
                                                                                                         aaGCTGAACCGTTGATGATGGTGTTCATCGGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGAGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCCTCCAGATATTCCATAATGATGc                                        <  1:1215673/149‑1 (MQ=255)
                                                                                                           gCTGAACCGTTGATGAGGGTGTGCATCAGCGTGTACCAGTCTTTTGGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCga                                      <  1:769444/149‑1 (MQ=255)
                                                                                                            cTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCgag                                     >  2:214417/1‑149 (MQ=255)
                                                                                                               aaCCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGAtt                                  >  2:522349/1‑149 (MQ=255)
                                                                                                               aaCCGTTGATGATAGGGTTCATCAGCGTGTACCAGTCATTTCTCTTTGATCACAGTGACGCCGGGGCCCGCGGCGAGCGACCGGTTAAACAGGCATCAGTGGCGGATGCGGGAGACGCTCATCAAGATATTCCATAATGCTGAGTGAtt                                  >  1:1166109/1‑149 (MQ=255)
                                                                                                               aaCCGTGGATGATGGTGTTCAGCAGCGTGTACCAGCCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGCAAACAGGCATCAGTGGCGGCTGCGGGGAGCGCACATCCAGACATTCCATAATGATGCGAGAtt                                  >  1:1142776/1‑149 (MQ=255)
                                                                                                                 ccGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTcc                                <  2:1466886/149‑1 (MQ=255)
                                                                                                                   gTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATACTCCATAAGGATGCGAGATTCCca                              <  1:968660/149‑1 (MQ=255)
                                                                                                                      gatgatGGTGTTCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAg                           <  2:1179119/149‑1 (MQ=255)
                                                                                                                       atgatgGTGTTCATCAGCGTGTACCAGTCTTTCCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTGCCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCGGATATTGCATAATGATGCGAGATTCCcacatg                          >  1:1308448/1‑147 (MQ=255)
                                                                                                                                ttCATCAGCGTGTACCGGTCTTTTCGATGCGGTGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa                 <  1:801716/149‑1 (MQ=255)
                                                                                                                                ttCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCCCCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCCTAATGATGCGAGATTCCCACAGGGTCAGCCCa                 >  2:975366/1‑149 (MQ=255)
                                                                                                                                ttCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACGGGGTCAGCTcc                 >  2:98997/1‑148 (MQ=255)
                                                                                                                                ttCATCAGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa                 <  1:97248/149‑1 (MQ=255)
                                                                                                                                tgcatcaGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa                 <  1:698888/147‑1 (MQ=255)
                                                                                                                                   atccGCGAGGACCAGTCTTTCTGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGGAACGCGCATCCAGATATTCCATAATGATGCGAGATTACCACCGGGTCAGCTCGCGa              >  1:365532/1‑149 (MQ=255)
                                                                                                                                   atcaGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCCCGa              >  2:1477683/1‑149 (MQ=255)
                                                                                                                                    tcaGCGTGTACCAGTCTTTTCGATGCGATGGATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGAt             <  1:468282/149‑1 (MQ=255)
                                                                                                                                    tcaGCGTGTACCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGTACCGCGCGTGCAGATATTCCATAATGATGCGCGCTTCCCACAGGGTCCGCTCCCGac             >  2:789868/1‑148 (MQ=255)
                                                                                                                                          tgtACCAGCCTTTTCGATGCGATGCATGTACAGACGGCCTGCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAAGGCTGCGAGATTCCCACAGGGTCAGCTCACGATccacca       <  1:1237142/149‑1 (MQ=255)
                                                                                                                                             aCCAGTCTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCAACGGCTCAGCTCGCGATCCAACgggg    >  1:623371/1‑149 (MQ=255)
                                                                                                                                               cAGTCTTCTCGGTGCGATGCATGTCCGGCCGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTc  <  2:1396231/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCGTTGATGATGGTGTTCATCAGCGTGTACCAGTCTTTTTCGATGCGATGCATGTACAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTC  >  CP009273/3370308‑3370600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: