Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,305,991 G→T Q80K (CAG→AAG)  ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,305,9910GT99.1% 355.8 / ‑5.2 106Q80K (CAG→AAG) ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (50/55);  minor base C (0/1);  total (50/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 106 total aligned reads displayed.

ACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAT  >  CP009273/2305849‑2306136
                                                                                                                                               |                                                                                                                                                 
aCGTCGGTCCAGGAAGTTACGTCATAAACAACGGCGTAGTTATGACCGAAGTCGAAAGAACCAACATCAGGACTTCTCAAAAACGTTACCCGGACACTGGTCCAGGAGTTGTTTTGGTTTTCAGCGCTGATGCCCTGGATCTTATATTc                                                                                                                                             <  2:1452580/149‑1 (MQ=255)
     ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTc                                                                                                                                             <  1:471203/144‑1 (MQ=255)
     ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTc                                                                                                                                             <  1:1407222/144‑1 (MQ=255)
     ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTACAGGAGGTGTTTTAGTTTTCCACGCTGTTGCCCTGGAACTTATATTc                                                                                                                                             >  1:232812/1‑144 (MQ=255)
      gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGCGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTcc                                                                                                                                            >  1:113315/1‑144 (MQ=255)
         cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGcc                                                                                                                                    <  2:261846/149‑1 (MQ=255)
           ggAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCCCTg                                                                                                                                       >  1:931218/1‑144 (MQ=255)
            gAATTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTgg                                                                                                                                      <  1:1166792/144‑1 (MQ=255)
             aaGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTaa                                                                                                                                >  2:1161318/1‑149 (MQ=255)
              aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGcc                                                                                                                                    <  1:618831/144‑1 (MQ=255)
                ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGGCCTGGATCTTATATTCCCACTGGCCGTAACCg                                                                                                                             >  2:1350616/1‑149 (MQ=255)
                ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCg                                                                                                                             <  2:1388068/149‑1 (MQ=255)
                  aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGt                                                                                                                           >  2:1375782/1‑149 (MQ=255)
                  aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTCATATTCCCACTGGCCGTAACCGGt                                                                                                                           <  2:1474549/149‑1 (MQ=255)
                   cGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTc                                                                                                                          <  2:339908/149‑1 (MQ=255)
                      cATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCgg                                                                                                                            >  1:637102/1‑144 (MQ=255)
                        tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGATCCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTg                                                                                                                     <  2:1106500/149‑1 (MQ=255)
                           acaacGCCGTAGTTACGACCGTAGTCGAAACAACCCACATCCTGGAATTTCAGACCTCCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTAGTGCTTATATTCCCCCTGGCCGTAACCGGTCAGc                                                                                                                       >  1:824660/1‑144 (MQ=255)
                            caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCt                                                                                                                      <  1:522134/144‑1 (MQ=255)
                             aacGCCGTAGTTACGGCCGGAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACAGGTGAGCTg                                                                                                                     <  1:18444/144‑1 (MQ=255)
                             aacGCCGTAGTTACGACCGTAGTCGAACGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCGGCTGGTCAg                                                                                                                <  2:328900/149‑1 (MQ=255)
                             aacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGCCAg                                                                                                                >  2:1502850/1‑149 (MQ=255)
                               cGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCACTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGt                                                                                                                   <  1:75200/144‑1 (MQ=255)
                                gCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTaa                                                                                                             >  2:125616/1‑149 (MQ=255)
                                 ccGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTCTCGTATTCAGCGCTGCTGGACTGGAGCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAAc                                                                                                            <  2:1951143/149‑1 (MQ=255)
                                   gTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCt                                                                                                          >  2:31530/1‑149 (MQ=255)
                                     gtaaccatcggTAGTCGAAAGAACCCACATCCTGGAATTTCACACCTGCGAATGCCACACGGGTTCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTag                                                                                                             <  1:457900/134‑2 (MQ=255)
                                      gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAg                                                                                                       <  2:334832/149‑1 (MQ=255)
                                          cGACCGTAGTCGAATGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGCCAGTAACCTGAGTTTc                                                                                                   <  2:1243765/149‑1 (MQ=255)
                                           gACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCa                                                                                                  <  2:614555/149‑1 (MQ=255)
                                           gACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATGCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAg                                                                                                       >  1:956366/1‑144 (MQ=255)
                                           gACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCGTATATTCCCACTGGCCGTCACCGGTCCGCTGGTCAGTAACCttgg                                                                                                       >  1:488664/1‑141 (MQ=255)
                                            gccGTAGTCGAAAGAACCCACAACCTGGAATTGTAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCGGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc                                                                                                 <  2:1628963/148‑1 (MQ=255)
                                               gTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTc                                                                                                   <  1:575527/144‑1 (MQ=255)
                                                tAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCa                                                                                                  >  1:988596/1‑144 (MQ=255)
                                                tAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCttt                                                                                             <  2:517211/149‑1 (MQ=255)
                                                   tCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGaa                                                                                          >  2:544367/1‑149 (MQ=255)
                                                   tCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGaa                                                                                          >  2:544194/1‑149 (MQ=255)
                                                   tCGAAAGAACCCACATCCTGGAATCTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGaa                                                                                          >  2:1472251/1‑149 (MQ=255)
                                                     gAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCttt                                                                                             >  1:628310/1‑144 (MQ=255)
                                                     gAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGc                                                                                        >  2:119104/1‑149 (MQ=255)
                                                     gAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGGCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCttt                                                                                             <  1:1312821/144‑1 (MQ=255)
                                                       aaGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGa                                                                                           <  1:6116/144‑1 (MQ=255)
                                                       aaGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCa                                                                                      <  2:1861785/149‑1 (MQ=255)
                                                        aGAACCCACCTCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCaa                                                                                     <  2:513316/149‑1 (MQ=255)
                                                        aGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGTATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCaa                                                                                     <  2:1217417/149‑1 (MQ=255)
                                                        aGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGaa                                                                                          <  1:270305/144‑1 (MQ=255)
                                                            cccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACg                                                                                 <  2:1627550/149‑1 (MQ=255)
                                                            cccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTGGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCa                                                                                      <  1:1574733/144‑1 (MQ=255)
                                                             ccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGc                                                                                <  2:1953217/149‑1 (MQ=255)
                                                             ccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGc                                                                                <  2:1953179/149‑1 (MQ=255)
                                                             ccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGc                                                                                <  2:1199242/149‑1 (MQ=255)
                                                             ccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATGCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGc                                                                                >  2:1344958/1‑149 (MQ=255)
                                                               acaTCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACTTTTGAAGCCAAGACGCAt                                                                              <  2:1455175/149‑1 (MQ=255)
                                                               acaTCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCAt                                                                              <  2:1573712/149‑1 (MQ=255)
                                                                   ccTGGAAGTTCAGACCTGCGAATGCCACCCGGGTCCAGGAGTTGTTGTCGTTTTCAGCGCTGTGGCCCTGGATCTTATATTCCCCCTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAg                                                                          <  2:1829268/149‑1 (MQ=255)
                                                                     tGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCATCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCGAGACGCATGGAGGt                                                                        >  2:69009/1‑149 (MQ=255)
                                                                      ggAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTc                                                                       >  2:1601765/1‑149 (MQ=255)
                                                                       gAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCt                                                                      <  2:244530/149‑1 (MQ=255)
                                                                           ttCACACCTGCGACTGCCACACGGGTCCAGGAGTTGTTCTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTc                                                                       <  1:1171120/144‑1 (MQ=255)
                                                                              cAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGc                                                                <  2:1970605/149‑1 (MQ=255)
                                                                               aGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGcc                                                               >  2:653960/1‑149 (MQ=255)
                                                                                gACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTCCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGt                                                                   <  1:975764/144‑1 (MQ=255)
                                                                                   cTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCt                                                           >  2:614247/1‑149 (MQ=255)
                                                                                    tGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGTGTTTCACCTTTTAAGCCAAGACGCATGTAGGTCTGGTCGcc                                                               <  1:838420/144‑1 (MQ=255)
                                                                                        aaTGCCTCACGGGTACAACAGCTTTATCCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCt                                                           >  1:844848/1‑144 (MQ=255)
                                                                                           gCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACa                                                        >  1:1150951/1‑144 (MQ=255)
                                                                                             cacaCGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATc                                                      >  1:72608/1‑144 (MQ=255)
                                                                                              acacGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCt                                                     >  1:158616/1‑144 (MQ=255)
                                                                                              acacGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGAATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCt                                                     >  1:617375/1‑144 (MQ=255)
                                                                                              acacGGGTCCAGGAGTTGTTTTCGTTTGCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgtt                                                <  2:1599953/149‑1 (MQ=255)
                                                                                              acacGGGTCCAGGAGTTGTTTTCGTATTCAGCGCTGTTGCCCTGGATCTTATATTCCCTCTGGCCGTAACCGGTCAGCTGGGCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgtt                                                <  2:1223249/149‑1 (MQ=255)
                                                                                                 cGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTc                                             <  2:509277/149‑1 (MQ=255)
                                                                                                   gggccAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgtt                                                >  1:1216231/4‑144 (MQ=255)
                                                                                                   ggTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTCGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCag                                           <  2:550633/149‑1 (MQ=255)
                                                                                                       cAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCa                                            >  1:1329909/1‑144 (MQ=255)
                                                                                                        aGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGGGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCag                                           >  1:13017/1‑144 (MQ=255)
                                                                                                          gAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATa                                    <  2:1274005/149‑1 (MQ=255)
                                                                                                          gAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCCTCGTTGTCcgag                                         >  1:250255/1‑144 (MQ=255)
                                                                                                                  ttcgttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTg                                 <  1:1625368/144‑1 (MQ=255)
                                                                                                                      ttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTGACCTGAGTTTCACCTTTGAAGCCAAGACGCATGAAGGTCTGGTCGCCATCTACATCTTGGTTGTCAGAGAAATGGTGCAgg                             >  1:1835563/1‑144 (MQ=39)
                                                                                                                      ttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGGAGGCCGTc                        >  2:592281/1‑149 (MQ=255)
                                                                                                                       tttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGAATGCTGGGCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                       >  2:1355193/1‑149 (MQ=255)
                                                                                                                           aGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCGGGCCGTc                        >  1:1545259/1‑144 (MQ=255)
                                                                                                                           aGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTc                        >  1:1582029/1‑144 (MQ=255)
                                                                                                                           aGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACtt                   <  2:1658313/149‑1 (MQ=255)
                                                                                                                            gcgcTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAAAAGTGCAGGCCGTCt                       >  1:288795/1‑144 (MQ=255)
                                                                                                                             cgcTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTa                      <  1:108305/144‑1 (MQ=255)
                                                                                                                             cgcTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTa                 >  2:292258/1‑149 (MQ=255)
                                                                                                                              gctgctGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTAc                     <  1:1475709/144‑1 (MQ=255)
                                                                                                                              gcTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTAc                     <  1:79158/144‑1 (MQ=255)
                                                                                                                                tGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGAGCAGGCCGGCTACGTTACCg              >  2:1426949/1‑149 (MQ=255)
                                                                                                                                    gCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGATGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCATCTACTTTAcc               >  1:1225228/1‑144 (MQ=255)
                                                                                                                                    gCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAcc               <  1:28213/144‑1 (MQ=255)
                                                                                                                                    gCCCTGGATCTTATATCCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCAAccggagc               <  1:17992/144‑7 (MQ=255)
                                                                                                                                      ccTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa        >  2:1413729/1‑149 (MQ=255)
                                                                                                                                       cTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGAt       >  2:1756426/1‑149 (MQ=255)
                                                                                                                                            tCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGCGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt  >  2:839725/1‑149 (MQ=255)
                                                                                                                                            tCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGAt       >  1:727621/1‑144 (MQ=255)
                                                                                                                                            tCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGGTTCACCTTTGAAGCCAAGCCGCTTGTAGGTCTGGTTGCCATCTACATCTTTGTTGTCAGAGAAATATTGCAGGCCGTCTACTTTACCGTCCAGCTCTAAt  >  2:1559206/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
ACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAT  >  CP009273/2305849‑2306136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: