Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,563,861 G→T L296M (CTG→ATG)  gadC ← glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,563,8610GT97.5% 377.1 / NA 119L296M (CTG→ATG) gadCglutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (67/48);  total (69/50)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

AAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCAGTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTT  >  CP009273/1563717‑1564003
                                                                                                                                                |                                                                                                                                              
aaaTGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCGTTGCg                                                                                                                                            >  2:1030380/1‑149 (MQ=255)
 aaTGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCgg                                                                                                                                           <  2:304691/149‑1 (MQ=255)
  ctGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGga                                                                                                                                          <  2:1813434/148‑1 (MQ=255)
  aTGACCAGCTTTCCCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGga                                                                                                                                          <  2:1800363/149‑1 (MQ=255)
  aTGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGga                                                                                                                                          >  2:253338/1‑149 (MQ=255)
  aTGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGga                                                                                                                                          <  2:1079294/149‑1 (MQ=255)
    gACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGgaga                                                                                                                                        <  2:1570172/149‑1 (MQ=255)
     aCCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCACTACGgagct                                                                                                                                       >  2:361081/1‑147 (MQ=255)
       cAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGga                                                                                                                                          >  1:326916/1‑144 (MQ=255)
       cAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCa                                                                                                                                     >  2:625342/1‑149 (MQ=255)
       cAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAAAACACCCAGCAACAGCATTGCGGAGATCa                                                                                                                                     >  2:1182444/1‑149 (MQ=255)
        aGCGGTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCAc                                                                                                                                    <  2:111615/149‑1 (MQ=255)
          cGTTCCCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGAt                                                                                                                                       <  1:1122094/144‑1 (MQ=255)
            ttACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCAcgcgg                                                                                                                                >  2:199784/1‑148 (MQ=255)
            ttACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGa                                                                                                                                >  2:892489/1‑149 (MQ=255)
              aCCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAAc                                                                                                                              >  2:1745851/1‑149 (MQ=255)
              aCCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAAc                                                                                                                              >  2:1746449/1‑149 (MQ=255)
                cGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATGCATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCGGAACGCCCAGCAACAGCATTGCGGAGATGACGCGAACCg                                                                                                                            >  2:1153916/1‑149 (MQ=255)
                  gTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTGCATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTc                                                                                                                          >  2:1318645/1‑149 (MQ=255)
                  gTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGaa                                                                                                                               >  1:1169112/1‑144 (MQ=255)
                     cGCAATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCAc                                                                                                                       <  2:614638/149‑1 (MQ=255)
                        cATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCa                                                                                                                    >  2:1762416/1‑149 (MQ=255)
                         aTTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCaa                                                                                                                   <  2:629290/149‑1 (MQ=255)
                          tttttGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCAc                                                                                                                       <  1:1258579/144‑1 (MQ=255)
                          tttttGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACAGACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCAc                                                                                                                       <  1:884115/144‑1 (MQ=255)
                           ttttGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAAtt                                                                                                                 <  2:1750156/149‑1 (MQ=255)
                                ttCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGCTTTACGCTGTTACACGCAGCAACACCATTGCGGAGATCACGCGAACCGTCACCTCAGTTTGTgg                                                                                                            >  2:464539/1‑149 (MQ=255)
                                 tCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCGCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATTACGCGAACCGTCCACTCAATTTCTGGt                                                                                                           >  2:1132986/1‑149 (MQ=255)
                                  cATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAGTCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCGCGCGAACGGTGCGCTCAATTTc                                                                                                               >  1:1623325/1‑144 (MQ=255)
                                  cATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTg                                                                                                          >  2:196200/1‑149 (MQ=255)
                                     tttAGCGAAGGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCca                                                                                                       <  2:538587/149‑1 (MQ=255)
                                      ttAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCcac                                                                                                      >  2:1398733/1‑149 (MQ=255)
                                          cGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCca                                                                                                       >  1:1095191/1‑144 (MQ=255)
                                          cGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATgg                                                                                                  >  2:169496/1‑149 (MQ=255)
                                           gAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATggg                                                                                                 >  2:469556/1‑149 (MQ=255)
                                            aaTGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGa                                                                                                >  2:631910/1‑149 (MQ=255)
                                                 cGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCTTTGCGGGGATCACGCGAACCGTCCACTCATTTTCTGGTGCCACCTGGGa                                                                                                >  1:1628041/1‑144 (MQ=255)
                                                  gCTGGCAGCAGGTTTTTCTGCGCTGTTACATACCTCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCTACATGGGAc                                                                                               <  1:842720/144‑1 (MQ=255)
                                                        agcagGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGa                                                                                         <  1:232561/144‑1 (MQ=255)
                                                           agGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTaaa                                                                                 >  2:809833/1‑149 (MQ=255)
                                                             gTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAgg                                                                               >  2:1080257/1‑149 (MQ=255)
                                                             gTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAAAGCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAgg                                                                               >  2:1148891/1‑149 (MQ=255)
                                                                 ttatGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCAACTCAATTTCTGGTGCCACATGGGAACTCAGAACGGTAAAg                                                                                >  1:502074/4‑144 (MQ=255)
                                                                  tCTGCGCTGTTACATACATCCCGCGCGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGTGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGc                                                                          <  2:1222755/149‑1 (MQ=255)
                                                                   cTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGt                                                                              >  1:466216/1‑144 (MQ=255)
                                                                    tGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGCAAAGGtt                                                                             >  1:1539861/1‑144 (MQ=255)
                                                                    tGCGCTGTGACAGACAGCGCGCGAGAAGGACCAACTTCCTAGGATGCAACTCCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCAt                                                                        <  2:1348038/149‑1 (MQ=255)
                                                                      cgcTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTg                                                                           >  1:1612324/1‑144 (MQ=255)
                                                                         tGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGCTCACGCGAACCGTCCACTCAATTTCAGGTGCCACATGGGACATCAGAACGGTAAAGGtaggcagtgccc                                                                   >  2:336817/1‑138 (MQ=255)
                                                                           ttACATACATACCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTa                                                                      <  1:529733/144‑1 (MQ=255)
                                                                            tacatacaTCCCGCGAGAAGGACCAACAATCCAGAAGGCGCTTTCCGCCAAAACACCCAGCAACAACATTGCGGCGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGGAAAGGTTTGCATTAc                                                                     >  1:878148/1‑144 (MQ=255)
                                                                             acatacatCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACCCAATTTCTCGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTAcc                                                                    >  1:1138581/1‑144 (MQ=255)
                                                                              catacatCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTAccc                                                                   >  1:1767956/1‑144 (MQ=255)
                                                                               atacatCCCGCGAGAAGGACCAACAATCCAGGAGGCGCTTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAGAAGTCTGCATTAcccc                                                                  >  1:1291079/1‑144 (MQ=255)
                                                                               atacatCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCACCAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGCACGGTAAAGGTTTGCATTAcccc                                                                  >  1:1040633/1‑144 (MQ=255)
                                                                                  caccccGCGAGAAGGACCAACAAGCCAGGAGGCGATTTCCGCCAGAACACCACGCAACAGCAGTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGgaga                                                          <  2:951564/146‑1 (MQ=255)
                                                                                   atCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCg                                                              >  1:138733/1‑144 (MQ=255)
                                                                                     cccGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGACAGGAAAAGGTTTACATGACCACCCCGGTGAGGt                                                       >  2:1929140/1‑149 (MQ=255)
                                                                                      ccGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGGGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTCTGCATTACCCCTGCGgag                                                           >  1:1068229/1‑144 (MQ=255)
                                                                                       cgcgAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGTACATCAGAACGGTAAAGGTTTGCATCACCCCTGCGgaga                                                          <  1:1105930/144‑1 (MQ=255)
                                                                                        gcgAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGAACACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGa                                                    >  2:1256414/1‑149 (MQ=255)
                                                                                         cgAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAgg                                                        <  1:179818/144‑1 (MQ=255)
                                                                                           agaAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGtt                                                      <  1:327635/144‑1 (MQ=255)
                                                                                            gaAGGACCAACAATCCCGGAGGCCATTCCCGCCACAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCCCTCAATTTCTGGGGCCACATGGGACATCAGACCGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTc                                                <  2:1306328/149‑1 (MQ=255)
                                                                                            gaAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTc                                                <  2:1274886/149‑1 (MQ=255)
                                                                                             aaGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCa                                               <  2:1625896/149‑1 (MQ=255)
                                                                                             aaGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTGGa                                                    >  1:607642/1‑144 (MQ=255)
                                                                                             aaGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACACAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGAAATCAGAACGGAAAAGGTTTGCATTACCCCTGCGGAGAGGTTGa                                                    >  1:1051813/1‑144 (MQ=255)
                                                                                               ggACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGAtt                                                  >  1:1214383/1‑144 (MQ=255)
                                                                                                   caacaaTCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTAccc                                         >  2:1850923/1‑149 (MQ=255)
                                                                                                    aacaaTCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGCTGATTTCAtt                                             >  1:1834634/1‑144 (MQ=255)
                                                                                                     acaaTCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGCGAGGTTGATTTCATTACCCgg                                       >  2:812466/1‑149 (MQ=255)
                                                                                                       aaTCCAGGAGGCGAGTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCCATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGaa                                     <  2:382915/149‑1 (MQ=255)
                                                                                                        aTCCGGGAGGCGATTTGCGCCTGTGCACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCCGGTGCCACATGAGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAAt                                    <  2:453534/149‑1 (MQ=255)
                                                                                                         tCCAGGCGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCg                                        >  1:669838/1‑144 (MQ=255)
                                                                                                         tCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATg                                   >  2:1213323/1‑149 (MQ=255)
                                                                                                         accAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCg                                        <  1:621181/143‑1 (MQ=255)
                                                                                                          ccAGGAGGCGGTTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGa                                  <  2:505931/149‑1 (MQ=255)
                                                                                                           ctggaggCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGATAGCAGTACCCCTGCGGAGAGGTTGATTTCATTACCCGGa                                      <  1:396011/142‑1 (MQ=255)
                                                                                                            aggaggCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGAcc                                >  2:1810921/1‑149 (MQ=255)
                                                                                                              gaggCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGAAATCATCACCCCGAATg                                   >  1:287215/1‑144 (MQ=255)
                                                                                                                ggCGATTTCCGCCAGAACCCCCCGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTCCCCCTGCGGAGCGGTTGATTTCATTTCCCGGAATGAc                                 <  1:167832/144‑1 (MQ=255)
                                                                                                                ggCGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCACTTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTCTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCg                            >  2:1865711/1‑149 (MQ=255)
                                                                                                                  cGATTTCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGGGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGGGGATGATTTCATTACCCGGAATGACCATCGCa                          >  2:470384/1‑149 (MQ=255)
                                                                                                                     tttCCGCCAGAACACCCAGCAACAGCAGTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACAGCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATa                       <  2:37051/149‑1 (MQ=255)
                                                                                                                      ttCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAg                      >  2:1739001/1‑149 (MQ=255)
                                                                                                                      ttCCGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGCTGATTTCATTGCCCGGACTGGCCATCGc                           >  1:665289/1‑144 (MQ=255)
                                                                                                                         cGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCaaa                        >  1:1039716/1‑143 (MQ=255)
                                                                                                                         cGCCAGAACACCCAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACa                   >  2:1240337/1‑149 (MQ=255)
                                                                                                                          gCCAGAACACCCAGCAACAGCCTTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGAACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATATACaa                  >  2:1344348/1‑149 (MQ=255)
                                                                                                                          gCCAGAACACCCAACAACAGCATTGCGGAGATCCCGCGAGCCGTCCCCTCACGGCGTAGGACCACACGGGACATCAGAACGGTAAAGGTTTGCATTACACCTGCGGAGAGGTGGATTTCATTACCCGGAATGACAATAGCACTa                       >  1:1108026/1‑144 (MQ=255)
                                                                                                                              gAACACCCAGCAACAGCATTGCGGAGATCACGCGCCCCGTCCGCTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAacca              >  2:1957065/1‑149 (MQ=255)
                                                                                                                                 cacCCAACAACCGGCTTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAac                <  1:248941/144‑1 (MQ=255)
                                                                                                                                   cccAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAacca              >  1:1639554/1‑144 (MQ=255)
                                                                                                                                   cccAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAAc         <  2:1229127/149‑1 (MQ=255)
                                                                                                                                    ccAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAaccac             <  1:332957/144‑1 (MQ=255)
                                                                                                                                    ccAGCAACAGCATTGCGGAGAGCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGGTTGCATTACCCCTGCGGAGCGGTTGATTTCATTACCCGGAATGACGATCGCAATAGACACACCACCAACa        >  2:921119/1‑149 (MQ=255)
                                                                                                                                     cAGCAACAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAaccacc            >  1:473728/1‑144 (MQ=255)
                                                                                                                                          aCAGCATTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAAACAAACCACCAACAGAGCtt  <  2:1503633/149‑1 (MQ=255)
                                                                                                                                           ccgcatTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACaga      <  1:433598/142‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
AAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACATCCCGCGAGAAGGACCAACAATCCAGGAGGCGATTTCCGCCAGAACACCCAGCAACAGCAGTGCGGAGATCACGCGAACCGTCCACTCAATTTCTGGTGCCACATGGGACATCAGAACGGTAAAGGTTTGCATTACCCCTGCGGAGAGGTTGATTTCATTACCCGGAATGACCATCGCAATAGACAAACCACCAACAGAGCTT  >  CP009273/1563717‑1564003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑