Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	CP009273	2,160,620	IS5 (+) +4 bp	intergenic (+110/+160)	yegQ → / ← ogrK	putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	2095230 =	NA (NA)	54 (0.990)	42/292	0.3	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	2160620 =	0 (0.000)					intergenic (+110/+163)	yegQ/ogrK	putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)
*	?	CP009273	= 2096424	NA (NA)	40 (0.730)	38/292	0.5	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	CP009273	= 2160623	0 (0.000)					intergenic (+113/+160)	yegQ/ogrK	putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)

AAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATAT  >  CP009273/2096287‑2096568                                                                                                                                          |                                                                                                                                                 cactctcTTTTCTGGCCTGACGGCGCTTACTGCTGAATTCACGGTCGGCGAAGGCAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGCTCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTaa                                                                                                                                       <  1:248021/146‑1 (MQ=11) aaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGCAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTaa                                                                                                                                       <  1:175271/149‑1 (MQ=21)               gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACaa                                                                                                                         <  1:104487/149‑1 (MQ=255)                tCTGACGGCGCTTACTGGTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGGACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCGTCCTTAATCCCTAACATAAATGac                                                                                                                             >  2:625018/1‑144 (MQ=255)                  tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGGCTCAGTCTCACCTTCCTTAATCCCTAACATAAATGACACAATaa                                                                                                                      >  1:402547/1‑149 (MQ=17)                  tGACGGCGCTTACTGCTGAATTCACCGTCGGAGCAGGTATGTTGATGACTCATGATGAACACTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGaaac                                                                                                                           >  2:601378/1‑144 (MQ=14)                  ggACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGacac                                                                                                                           >  2:584329/2‑144 (MQ=255)                    aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGCTGCACCCTGTTCTATGACTCCCGATGACAAACAGGATTTCTTATCAGGTACTTCTGCGCACCTTCCTTATTCCCTAACATAAATGACACaa                                                                                                                         >  2:489358/1‑144 (MQ=14)                         gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGAACTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATATATGACACAATaaaa                                                                                                                    <  2:623475/144‑1 (MQ=255)                                gctcAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAGCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCACATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGaaa                                                                                                             <  2:748521/144‑1 (MQ=255)                                   gAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATACAGGACACAATAAAATCAGAAAGca                                                                                                          >  2:815534/1‑144 (MQ=255)                                    aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGaa                                                                                                    >  1:813434/1‑149 (MQ=255)                                     aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGaaa                                                                                                   >  1:648104/1‑149 (MQ=255)                                          ccgTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTc                                                                                              <  1:871446/147‑1 (MQ=255)                                           tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACACTAAAATCAGAAAGCACAGGAAATTTTCt                                                                                             <  1:495225/149‑1 (MQ=255)                                            gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAAtt                                                                                                 >  2:293731/1‑144 (MQ=255)                                                      gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCAACGGAAATTTGCTTACGGa                                                                                       >  2:856467/1‑144 (MQ=255)                                                       tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATc                                                                                 <  1:267748/149‑1 (MQ=255)                                                           ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGaataat                                                                                  >  2:696022/1‑144 (MQ=255)                                                                    cATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATAATGTAAAg                                                                         >  2:700447/1‑144 (MQ=255)                                                                     ctgatgaACCCTGTTCTATGGCTCCAGCGGGCAAGCATGAGCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGATAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCg                                                                   <  1:78568/148‑1 (MQ=37)                                                                         tgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTGCGGAATAATCATGTAAAGATTTCGCTTc                                                               <  1:511557/149‑1 (MQ=255)                                                                           aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGc                                                                  >  2:577987/1‑144 (MQ=255)                                                                                    cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCg                                                         >  2:54958/1‑144 (MQ=255)                                                                                    cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCg                                                         >  2:54957/1‑144 (MQ=255)                                                                                    cTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCg                                                         >  2:54883/1‑144 (MQ=255)                                                                                     tATGGCTCCAGATGACAAACATGCTCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTCAGGAATAATCATGTAACGATTTCGCTTCACCTCGGAACAg                                                   >  1:843471/1‑149 (MQ=37)                                                                                                     aaaCATGATCTCATATCAGGGCCTACCAAGCGCCGTCCTTAATCGCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCACGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTAc                                        >  2:96452/1‑144 (MQ=255)                                                                                                     aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATACTGtcc                                        >  2:26503/1‑142 (MQ=255)                                                                                                     aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATCTat                                        >  2:327491/1‑143 (MQ=255)                                                                                                        cATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCa                                >  1:784369/1‑149 (MQ=255)                                                                                                           gATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATGATGTACTAAAAGCATaa                             >  1:538611/1‑149 (MQ=255)                                                                                                           gATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATaa                             >  1:538511/1‑149 (MQ=255)                                                                                                             tctcATATCAGGGAGTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACg                           <  1:802461/149‑1 (MQ=255)                                                                                                                  tatCAGGGACTTGTCCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGCCATAATGTACTAAAAGCATAACGCTTAc                      <  1:496884/149‑1 (MQ=255)                                                                                                                   atCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACt                     >  1:726253/1‑149 (MQ=255)                                                                                                                    tCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACtt                    >  1:501819/1‑149 (MQ=255)                                                                                                                       gggACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAAGCAGAAAGCACAGGAAATTTTCTTAAGGAATAATCATGTAAAGATTTCGCTTCATCGCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCg                 <  1:131112/149‑1 (MQ=255)                                                                                                                                  gCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAat      >  1:657460/1‑149 (MQ=255)                                                                                                                                   cACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATAtt          >  2:801128/1‑144 (MQ=255)                                                                                                                                      cttccttAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAatatat  <  1:735845/149‑1 (MQ=255)                                                                                                                                       ttccttAATCCCTAACATTAATGACACAATAAAATCAGCTGGCCCAGGTGATTGTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAat      <  2:818171/144‑1 (MQ=255)                                                                                                                                          cttCATCCCTAGCATGAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAatata   >  2:517384/1‑144 (MQ=255)                                                                                                                                          |                                                                                                                                                 AAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAATCCCTAACATAAATGACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATAT  >  CP009273/2096287‑2096568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.