BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	982,560	T→A	intergenic (‑122/+481)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	982,560	0	T	A	100.0%	121.6 / NA	39	intergenic (‑122/+481)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (24/15); total (24/15)

TGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAC  >  CP009273/982415‑982704

tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCa                                                                                                                                               <  2:923146/149‑1 (MQ=255)

    aGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGGTTGGTGTCTTTATGTTGCTGCAGGGATCTTACCATTCa                                                                                                                                           >  1:291021/1‑149 (MQ=255)

                ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTc                                                                                                                               >  1:969749/1‑149 (MQ=255)

                    tcatTATTTATTACCCTCATGGTTTTTTTTTTTTTTTTTGCCACTGGCGTCAATAAGATCTGTCAATTAAAATATACGGAACTATTGATGGGAGTTTGGTGTATTTATTTGTTTGCAGCCATCTTACTATTTATACTAATTTTTcccca                                                                                                                           >  2:185710/1‑145 (MQ=255)

                    tcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTa                                                                                                                           >  1:937141/1‑149 (MQ=255)

                      attattTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAGTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTGTGTGTCTTCAGGCATCTTAACATTCAAACTAACGTTTCGCTTcc                                                                                                                         >  1:597425/1‑149 (MQ=255)

                      attattTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCTTTCAAACTAACGTTTCGTTAcc                                                                                                                         >  1:72175/1‑149 (MQ=255)

                       ttatttatTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCg                                                                                                                        <  1:912327/149‑1 (MQ=255)

                        tatttattCCCCTCAGGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGt                                                                                                                       <  1:834345/149‑1 (MQ=255)

                            tactcaccTCAGTGTTTTTTCTTGGACACCTGCCACGGCCGTCAATAAGTTCTGTCAATAAAAAGTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGaaa                                                                                                                   <  2:777083/143‑1 (MQ=255)

                                                  aTGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTg                                                                                             >  1:794897/1‑149 (MQ=255)

                                                   tGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgg                                                                                            <  2:128521/149‑1 (MQ=255)

                                                   tGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAACGATTTgg                                                                                            >  2:6861/1‑149 (MQ=255)

                                                     acacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGtt                                                                                          >  1:750097/1‑149 (MQ=255)

                                                     acacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAAATTTGGtt                                                                                          >  2:726144/1‑149 (MQ=255)

                                                       acCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc                                                                                        <  2:199790/149‑1 (MQ=255)

                                                            ccACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaaa                                                                                   >  1:796119/1‑149 (MQ=255)

                                                             cccggccgcaaaTTAGTTCTGGCAATAAAAATTTACGGAATTATTTATGAGAGTTTGGTGTTGTTATTTGTCTGCAGGTATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTTCTACAAAGATAAAGATTTGGTTTCAAAAAg                                                                                  <  2:28119/139‑1 (MQ=255)

                                                             cACTGCCGTCAATAAGTTCTGTCATTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg                                                                                  >  1:744107/1‑149 (MQ=255)

                                                                tGCCTTCAATAATTTCTGGCAATAAAAATTTACGGAACTATTGATGAGAGTGTGGTGTCTTTATGTGTCTGCATGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaa                                                                               <  2:388420/149‑1 (MQ=255)

                                                                         tAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTa                                                                      >  1:408057/1‑149 (MQ=255)

                                                                            gTTCTGTCAATAAAAATCTCTGGCACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCAGCTTACCATTAAAACTAACGTTTCGCTAACGTGAAAGTGCGACAAAGATAAAGATCTGGTTTCAAAAAGAAAAAATATGTAAcc                                                                   <  2:917675/149‑1 (MQ=255)

                                                                              tCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaa                                                                 <  2:750097/149‑1 (MQ=255)

                                                                              ccTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGGCTGCAGGCATCTTACCATTCAAACCAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaa                                                                 <  1:404699/148‑1 (MQ=255)

                                                                                     aTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATTTAACCAAAAGTaaa                                                          >  2:55299/1‑149 (MQ=255)

                                                                                     aTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaa                                                          >  1:464347/1‑149 (MQ=255)

                                                                                                 ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCGAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                              >  2:640794/1‑149 (MQ=255)

                                                                                                 ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                              >  1:499547/1‑149 (MQ=255)

                                                                                                     cTATTGATGGGGATTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                          <  1:376001/149‑1 (MQ=255)

                                                                                                     cTATTGATGGGAGTTTGGTGTCTTTATGTGTGTGCAGGCATCTTACCCTTCAAACTGACGTTACGCTACCATGAAAGTGCTACCAAGGTAAGGATTGTTTTTCATGAAGAGAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                          <  2:291021/149‑1 (MQ=255)

                                                                                                      tATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg                                         >  1:223204/1‑149 (MQ=255)

                                                                                                       aTTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                        >  1:982770/1‑149 (MQ=255)

                                                                                                       aTTGATGAGAGTTTCATGTCTTTATGTGTCTGACGGCATTATACCATTCGAACTAACGTTTCGCTACCGTGAAAGGGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                        >  1:738902/1‑149 (MQ=255)

                                                                                                                      gtctCTTTATGTGTCTGCAGGCATCTTACCATTCTAACTAACGTTTCGCTACCATGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTc                         <  2:453299/146‑1 (MQ=255)

                                                                                                                       tgtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcc                        >  1:333876/1‑149 (MQ=255)

                                                                                                                        gtCTTTATGTGTCTGCAGGCATCTTACCCTTCACCCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAACGTAAAATTTAAGGAACTTTGTAAAAACCGTCACATTTCCa                       >  2:218677/1‑149 (MQ=255)

                                                                                                                        gtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa                       >  2:978965/1‑149 (MQ=255)

                                                                                                                                     tgcagggtgcTTACCATTCAAACTACCGTTTCGATACCGTGAAAGTGCTACAAATATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg          <  2:882249/140‑1 (MQ=255)

                                                                                                                                             tCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAc  >  2:459189/1‑149 (MQ=255)

TGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAC  >  CP009273/982415‑982704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`