Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 444,779 C→T W440* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,7790CT96.4% 156.9 / NA 57W440* (TGG→TAG) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (28/26);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCA  >  CP009273/444634‑444920
                                                                                                                                                 |                                                                                                                                             
ttCAATCTGCTCGCCCAAAAGACGGGTCATGCCTATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGctacc                                                                                                                                            <  2:44392/148‑1 (MQ=255)
       tgctgGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCAGAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGCTATTGGTGAAACCGAACGCTTTAGGCTACCAGTAGGt                                                                                                                                     <  2:219244/149‑1 (MQ=255)
       tgctgGCTCAAACGACGGGTCATGCCCAGGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGt                                                                                                                                     <  1:75676/149‑1 (MQ=255)
          tgGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCAt                                                                                                                                  >  1:445169/1‑149 (MQ=255)
              tCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTCGCATAAAGGCAACGAAGAAGCCGACGATCCAGAACCAGAACGCGCGCTTACCCCAGGCTTCGCTCAGATTGAAACCGAACGCTACAGGCTCCCAGTAGGTCAGcccc                                                                                                                              >  2:685438/1‑148 (MQ=255)
                aaaCGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGGTTGAAACCGAACGCCTTAGGCTACCAGTAGGTCATCCCTGc                                                                                                                            >  2:18710/1‑149 (MQ=255)
                  acgacgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGa                                                                                                                          >  2:973353/1‑149 (MQ=255)
                   cgacgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCGCTACGCTACCAGTAGGTCATCCCTGCGaa                                                                                                                         <  1:990369/149‑1 (MQ=255)
                    gacgGGTCATGCCCATGAAGCCCAGCGCATAGAGTGGCATGAAGGCAGCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCGCAGGTTTCGTTCAGTTTGAAACGGAACGCTTTAGGCTACCAGTAGGTCATCACTGCGAag                                                                                                                        >  2:480890/1‑149 (MQ=255)
                    gacgGGTCATGCCCATGAAGCCCAGCGCATACAGGGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGAGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAag                                                                                                                        <  1:333881/149‑1 (MQ=255)
                     atggggCAGGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAGGCCGATGATCCAGAACCAGACCGCGCGGTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAagc                                                                                                                       <  2:99076/147‑1 (MQ=255)
                       gggTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGAACCAGAACCAGAACACGCGTTTACCCCAGGTTTCGGTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGCCATCCCTGCCGagcag                                                                                                                     >  2:821043/1‑149 (MQ=255)
                       gggTCATGCCCATGAAGCCCAGCGCAGACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAagcag                                                                                                                     >  2:758560/1‑149 (MQ=255)
                                ccATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGGTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGAcc                                                                                                            >  1:503508/1‑149 (MQ=255)
                                ccATGAAGCCCAGCGCATACAGTGGCATAAAGGACACGAAGAAGCCGATGATCCAGAACCAGAAGGCGCGTTCACCCCAGGTTTCGATCAGTTTGAAACCGCACGCTATAGGCTACCAGTCGGTCATCCCTGCGAAGCAGCCGagaacg                                                                                                            >  2:269776/1‑144 (MQ=255)
                                  aTGAAGCCCAGCGCATACAGGTGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAc                                                                                                          <  1:540494/149‑1 (MQ=255)
                                          cAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCga                                                                                                  >  1:698172/1‑149 (MQ=255)
                                              gcATACAGTGACATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCAAAGACCACGCCGCCgatgat                                                                                              >  1:594472/1‑149 (MQ=255)
                                               cATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCACGTTTCGTTCAGTTTGAAACCGAACGCTTTAGCCAACCAGTAGGTCATCCCAGCGAAGCAGCCGAAGACCACGCCGCCgacgcac                                                                                             >  2:122501/1‑144 (MQ=255)
                                                  aCAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGAGGATCACg                                                                                          >  2:307052/1‑149 (MQ=255)
                                                          aTAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGaa                                                                                  <  1:890411/149‑1 (MQ=255)
                                                               gggaaCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGGTATGGAAGTgcg                                                                             <  2:616957/146‑1 (MQ=255)
                                                               ggcaatcaacacgCCGATGACCCAGAATCAGAACGCGCGTTCACCCCAGGTTTCGCTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGGCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTgcg                                                                             <  1:124702/137‑1 (MQ=255)
                                                               ggCCACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCCGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCCCCAGTAGGTAAACCCTGCGAAGCAGCCGAAGACCAAGCCGCCGATGATCACGTTATGGAAGTgcg                                                                             <  2:366473/149‑1 (MQ=255)
                                                                      aagaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAg                                                                      <  2:932634/149‑1 (MQ=255)
                                                                        gaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGa                                                                    <  1:203864/149‑1 (MQ=255)
                                                                           gCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAAcg                                                                 >  2:933986/1‑148 (MQ=255)
                                                                           gCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACa                                                                 >  1:797892/1‑149 (MQ=255)
                                                                           gCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCGTTAGGCTACCCGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGAGGAGCCCGTTATGGAAGTGCGCAATCAgaggaa                                                                 >  1:87576/1‑144 (MQ=255)
                                                                            ccGATGCTCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAGCGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGCGCGCAATCCCGAACAg                                                                >  2:551200/1‑149 (MQ=255)
                                                                              gatgatCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCACTCAGGGACAGGc                                                              >  2:237280/1‑149 (MQ=255)
                                                                               aggccccagaaccagaaCGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCt                                                             <  2:586643/144‑1 (MQ=255)
                                                                                  atCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAATACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGtt                                                          <  2:553579/149‑1 (MQ=255)
                                                                                    ccagaaccagaaCGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTAt                                                        >  1:175659/1‑149 (MQ=255)
                                                                                    ccagaaccagaaCGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCGGTTAt                                                        >  1:1174/1‑149 (MQ=255)
                                                                                     cagaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATg                                                       <  2:315304/149‑1 (MQ=255)
                                                                                     cagaaccagaacGCGCGTTTAACCCAGGTTTCGTCCAGTTTGACACCGAACGCTATAGCCTACCAGTAGGCCACCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATg                                                       >  2:291142/1‑149 (MQ=255)
                                                                                      agaaccggaacGCGCGTCTACCCCAGGTTTAGCTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGc                                                      <  1:834/149‑1 (MQ=255)
                                                                                               acGCGCGTTTCCCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAg                                             <  2:992193/149‑1 (MQ=255)
                                                                                                   gcgTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCcg                                         >  1:551514/1‑149 (MQ=255)
                                                                                                     gTTTGCCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCcgcg                                       <  2:839913/149‑1 (MQ=255)
                                                                                                     gTTTCCCCCAGGGTTCGTGCAGTTTGAAACCGAACGCTATAGGGTAGCAGTAGGTCATCCCGGCGAAGCAGCCGAAGACCACGCCGCCGATGAGTACTTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCcgcg                                       <  2:356913/149‑1 (MQ=255)
                                                                                                     gTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCcgcg                                       <  2:445169/149‑1 (MQ=255)
                                                                                                           cccAGGTTTCGTTCAGTTTGAAACCGAAAGCTATAGGCTACCAGTACGTCATCCCGGCGAAGCAGCCGAGGACAACGCCGCCGCTGATCACGAGATGGAAGTGCGCAATCAGGAACAGGCGGTTAGACAGAACGAAGGAcgcggcggat                                 >  2:251471/1‑143 (MQ=255)
                                                                                                            ccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCATTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTa                                <  1:868886/149‑1 (MQ=255)
                                                                                                            ccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGGACACGCCGCCGATGGACTGGTTATGGAAGTGTGCAATGAGGAACAGGCTGTTATGCATGACGAGGTCCGCGCCGGGTa                                >  2:340045/1‑149 (MQ=255)
                                                                                                              atggttCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACg                              <  2:192945/145‑1 (MQ=255)
                                                                                                                gTTTCCTTCAGTTTGAAACCGAACCCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGc                            <  2:67775/149‑1 (MQ=255)
                                                                                                                      ttCAGTTTGAAACCGAACGCTTTAGGCTACCGGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCGATCAGGAACAGGCTGTTATGCAGAACGAAGCGCGCGCCCGGTACGGCccgcgg                      >  2:774644/1‑147 (MQ=255)
                                                                                                                       tCAGTGTGCAAACGAACGCTTCAGGCTATCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGttatgtgagtagc                     <  2:172904/149‑13 (MQ=255)
                                                                                                                            ttGAAACCGAACGCGATAAGCTACCAGTAGACACTGAATGGGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGcc                >  1:738099/1‑149 (MQ=255)
                                                                                                                             tGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCa               <  1:829469/149‑1 (MQ=255)
                                                                                                                             tGAAACCGAACGCCTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCa               <  1:335534/149‑1 (MQ=255)
                                                                                                                               aaaCCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGt             >  2:96688/1‑149 (MQ=255)
                                                                                                                                      aCGCTTCAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCg      <  1:237167/149‑1 (MQ=255)
                                                                                                                                          tttAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCGGTCATCCCGcccc  >  1:750060/1‑148 (MQ=255)
                                                                                                                                          tttAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCa  >  1:799070/1‑149 (MQ=255)
                                                                                                                                          tttAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCa  >  1:170860/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCA  >  CP009273/444634‑444920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑