Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,742,902 C→T 5.6% A104V (GCG→GTG)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,742,9020CT 5.6% 196.5 / 2.1 71A104V (GCG→GTG) sgbUputative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (29/38);  new base T (2/2);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

TTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGG  >  CP009273/3742759‑3743048
                                                                                                                                               |                                                                                                                                                  
ttCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGcgcgcg                                                                                                                                               >  1:321863/1‑149 (MQ=255)
   gCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGAtc                                                                                                                                            >  1:156539/1‑149 (MQ=255)
      ggTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCg                                                                                                                                         <  1:391457/149‑1 (MQ=255)
        ttGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGc                                                                                                                                       >  1:111848/1‑149 (MQ=255)
        ttGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGc                                                                                                                                       <  1:392074/149‑1 (MQ=255)
         tGCCGCGATGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCa                                                                                                                                      <  1:29875/149‑1 (MQ=255)
             gcgATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGTGCGCGATCTCGGCATTCg                                                                                                                                  >  1:297074/1‑149 (MQ=255)
             gcgATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGTGCGCGATCTCGGCATTCg                                                                                                                                  >  1:297016/1‑149 (MQ=255)
              cgATGATCGAAACAGGTGTTGGGATCCCGGCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCAGGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGc                                                                                                                                 <  2:142987/149‑1 (MQ=255)
                  gatCTAATCAGGTGTTGGTATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGTCGCGCGATCTCGGCATTCGCACCa                                                                                                                             <  2:453534/149‑1 (MQ=255)
                   atCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCAt                                                                                                                            >  2:337657/1‑149 (MQ=255)
                   atCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCAt                                                                                                                            >  1:29519/1‑149 (MQ=255)
                    tCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGGAATCATGAGTAAAGCGATTCGCCGGGCGCGCGATCTAGGCATTGGCACCATc                                                                                                                           >  1:218796/1‑149 (MQ=255)
                      gAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCa                                                                                                                         >  2:164194/1‑149 (MQ=255)
                         acagtggtTGGGACCCCGTCGATGTGCCTGTCCGCACACCGTCGCTTGCCCTTTGGTGGCCGTGACGAGGCAGGGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCt                                                                                                                      <  2:87072/143‑1 (MQ=255)
                           aGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTgg                                                                                                                    <  2:148726/149‑1 (MQ=255)
                             gtgtTGGGATCTCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGTATTCGCACCATCCAGCTGGCa                                                                                                                  <  1:331691/149‑1 (MQ=255)
                              tgtTGGGATCCCGTCGATGTGCCTGTAAGCGAAGTGGCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAg                                                                                                                 >  1:228012/1‑149 (MQ=255)
                                 tGGGTTCCCGTCCGGGGGCCTGTCCGCGCATCGCCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGGGCGCGAGCGGGCGCGGGAAACGCTCAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGtt                                                                                                              <  2:243952/149‑1 (MQ=255)
                                   ggATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTACCCGTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTAt                                                                                                            <  1:202482/149‑1 (MQ=255)
                                    gctCCCGGCGATGTGCCTTGCCGCACAGCGTCGTTTTCCCTTTGGCAGCCGGGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATg                                                                                                           <  2:196428/147‑1 (MQ=255)
                                      tCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGAc                                                                                                         >  2:364754/1‑149 (MQ=255)
                                         cGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTc                                                                                                      >  2:451254/1‑149 (MQ=255)
                                            cGATGTCCCTGCCCGCACGTCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGAATTCGCACCATCCAGCTGGCAGGTTATGACGTCTAt                                                                                                   <  2:332703/149‑1 (MQ=255)
                                              gtgcgCCTGTCCGCACGTCGTCGCTGTCCCTGTTGTAGCCGTGTCGTGGCAGTGCGCGAACGGGCGCGGGTCCTCATGGGTATCGCTATTCTCCCGGCGCGCGAGCTCGGCCTTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTa                                                                                                 <  2:115372/145‑1 (MQ=255)
                                                gtgCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACTATGCAGTGTGCGAACGGGCGCGGGAAATCGTGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACg                                                                                               <  1:243765/149‑1 (MQ=255)
                                                gtgCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACg                                                                                               >  1:63840/1‑149 (MQ=255)
                                                gtgCCTGGCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACg                                                                                               <  1:12574/149‑1 (MQ=255)
                                                 tgCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGa                                                                                              <  1:261141/149‑1 (MQ=255)
                                                   ccTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAg                                                                                            >  1:418544/1‑149 (MQ=255)
                                                      gTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGGGCGCGAACGGGCGCGGGAAAGCATGAGTAAAGCGATTCGCCTGGCGCGCGGTCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGGCTATTACGAAGAcg                                                                                         >  2:389644/1‑148 (MQ=255)
                                                         cGCACATCGTCGCTTTCCCTTTGGTAGCAGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCCTCCAGCTGGCAGGTTATGACGTCGATTACGAAGACCacg                                                                                      >  1:249098/1‑149 (MQ=255)
                                                          gCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGACAGGTTATGACGTCTATTACGAAGACCacga                                                                                     >  2:65587/1‑149 (MQ=255)
                                                             cttcgtcgCTTTCCCTTTGGTAGGCGTGCCGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCAcgacga                                                                                  <  1:136728/147‑1 (MQ=255)
                                                              agcgtcgcggctccTTTGGGAGCCGTGACGAGGCAGTGCGCGATCGGGCGCGGGAAGTCTTGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGaa                                                                                 <  2:130440/137‑1 (MQ=255)
                                                                cgtcgCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCCGGAAAGCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAgg                                                                               <  1:204585/149‑1 (MQ=255)
                                                                        tCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGc                                                                       >  1:26823/1‑149 (MQ=255)
                                                                                gTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGCCGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGtttt                                                               <  1:95150/149‑1 (MQ=255)
                                                                                 tAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGTGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTg                                                              <  2:297074/149‑1 (MQ=255)
                                                                                 tAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGTGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTg                                                              <  2:297016/149‑1 (MQ=255)
                                                                                  aGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGc                                                             <  2:111848/149‑1 (MQ=255)
                                                                                    ccGTGACGAGGCAGTGCGCGCACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTg                                                           <  1:62552/149‑1 (MQ=255)
                                                                                      gggACGAGGCGGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGaa                                                         <  2:421939/147‑1 (MQ=255)
                                                                                        gACGAGGCCGGGCGCGAACGGGCCCGGGAAACCATGAGTAAATCGCTTCGCCTGGCGCGCGATCTCGGCCTTCGCACCATCCAGCGGGCAGGTTCTGACGTCTACTACGAAGCCCACGACGAAGGCACCCGGCAACGTTTTGCTGAAgg                                                       <  2:248781/149‑1 (MQ=255)
                                                                                        gACGAGGCAGTGCGCGAACGGGCGCGGGCAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACAACGACGAAGGCACCCGGCAACGTGTTGCTGAAgg                                                       >  1:246688/1‑149 (MQ=255)
                                                                                            aGGCCGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTg                                                   <  2:404149/149‑1 (MQ=255)
                                                                                                 gTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGGAGGGCTGGCgcg                                              >  2:69896/1‑147 (MQ=255)
                                                                                                  tGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTgg                                             <  2:156539/149‑1 (MQ=255)
                                                                                                   gcgcGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGCCGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTggg                                            <  1:20276/149‑1 (MQ=255)
                                                                                                    cgcgAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGc                                           >  1:432293/1‑149 (MQ=255)
                                                                                                           gggCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGaa                                    <  2:29519/149‑1 (MQ=255)
                                                                                                             gcgcGGGAAATCATGCGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACa                                  <  1:370916/149‑1 (MQ=255)
                                                                                                             gcgcGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCGCCCAGCTGGCCGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCACCGTTTTGCGGAAGGGCTGGCGTGGGCAGTCGACCa                                  >  1:97130/1‑149 (MQ=255)
                                                                                                                   gagaTCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGACGGCACACGGCAACGTTTTGCTGACGGGCTGCCGTGTGCAGTCGCACAggcgac                            >  1:317200/4‑147 (MQ=255)
                                                                                                                   gAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAggcggc                            >  1:383732/1‑149 (MQ=255)
                                                                                                                    aaaTCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAggcggcg                           <  1:364754/149‑1 (MQ=255)
                                                                                                                        aattaGTAAAGCGAGTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCAt                       <  1:125407/145‑1 (MQ=255)
                                                                                                                         aTGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATc                      >  1:244879/1‑149 (MQ=255)
                                                                                                                             gTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCGGGCGTGGGCAGTCGAACAGGCGGCGGCATCACaa                  >  1:253338/1‑149 (MQ=255)
                                                                                                                             gTAAAGCGATTCGCCTGGCGCGCGATCTCAGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACaa                  >  1:286504/1‑149 (MQ=255)
                                                                                                                               aaaGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCGGAAGGGCTGGCGTGGGCAGTGCAACAGGCGCCGGCCTcaccgta                >  2:209374/1‑145 (MQ=255)
                                                                                                                                 aGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGCGCAGTCGAACAGGCGGCGGCATCACAAGTaa              >  1:314449/1‑149 (MQ=255)
                                                                                                                                    gATTCCCCTGCCGCGCGATCTCGGCATTCGCACCATCCAGCGGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGc           <  1:169338/149‑1 (MQ=255)
                                                                                                                                     aTTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCt          <  1:451254/149‑1 (MQ=255)
                                                                                                                                     aTTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGGAATGCt          >  1:383419/1‑149 (MQ=255)
                                                                                                                                         gCCTGGCGCGCGATCTCGGCATTCGCACCATCCGGCTGGCAGGTTCTGACGTCTATTACGAAGACCACGACGAAGGCACCGGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCg      >  2:185766/1‑149 (MQ=255)
                                                                                                                                         gCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCg      >  2:149744/1‑149 (MQ=255)
                                                                                                                                           ccggCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTCAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCCGCCTCACAAGTAACGCTGGCAGt    <  2:465314/147‑1 (MQ=255)
                                                                                                                                            tGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTg   <  2:228012/149‑1 (MQ=255)
                                                                                                                                            tGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTg   <  2:184618/149‑1 (MQ=255)
                                                                                                                                            tGGCGCGCGAGCGCGGCATTCGCACCGTCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTg   <  1:164194/149‑1 (MQ=255)
                                                                                                                                             ggCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTgg  <  2:414227/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACAGGCGGCGGCATCACAAGTAATGCTGGCGGTGG  >  CP009273/3742759‑3743048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑