Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,528,506 A→C 50.1% V241G (GTA→GGA)  envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,528,5060AC50.1% 6.1 / 33.3 32V241G (GTA→GGA) envZsensory histidine kinase in two‑component regulatory system with OmpR
Reads supporting (aligned to +/- strand):  ref base A (9/7);  new base C (9/7);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAAC  >  CP009273/3528364‑3528642
                                                                                                                                              |                                                                                                                                        
cAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCg                                                                                                                                    >  2:10004/1‑149 (MQ=255)
    tAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCAt                                                                                                                                >  1:263779/1‑149 (MQ=255)
          agaaaCTGCTCAAGGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATcagcag                                                                                                                          >  2:412986/3‑149 (MQ=255)
          aTAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCACcagcag                                                                                                                          >  2:179215/1‑149 (MQ=255)
            aaaCTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCg                                                                                                                        >  2:193307/1‑149 (MQ=255)
                tGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCg                                                                                                                    >  2:115212/1‑149 (MQ=255)
                  cTCAATGATTGCGTTGCACTCTTCGATATCTTGATTGTTCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCTAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGt                                                                                                                  <  2:95799/149‑1 (MQ=255)
                   tCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGGCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACCTCCCCCCGCCATcagacgccctgggtc                                                                                                                 >  2:190214/1‑137 (MQ=255)
                           ggCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCa                                                                                                         >  1:51960/1‑149 (MQ=255)
                           ggCGATGCCCTCTTCTGTCTCTTTATTGATCGATCCTGCCAGATAGCCATCCTGCTCGCTCATCATCCCAGTCGCCAGGCGAACACGCGCCAGCGGCGTGCGCAAGTCGTGCCTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCa                                                                                                         <  2:261937/149‑1 (MQ=255)
                                      ctTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAaca                                                                                              >  1:5093/1‑149 (MQ=255)
                                              gcTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCTCAAGTCGTGACTTACCCCCGCCATCAGCATCGTGCGGTCATCCGCCAGTTGCTTAACACCAgccgc                                                                                      <  2:199892/148‑1 (MQ=255)
                                                    ttGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATg                                                                                <  1:398631/149‑1 (MQ=255)
                                                               tGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCAc                                                                     >  1:388956/1‑149 (MQ=255)
                                                               tGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCAc                                                                     >  2:241652/1‑149 (MQ=255)
                                                                gCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACg                                                                    >  1:374265/1‑149 (MQ=255)
                                                                   aGATAGCCATCCTGCTCGCTCCTCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGt                                                                 <  1:340484/149‑1 (MQ=255)
                                                                   aGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGt                                                                 <  1:307876/149‑1 (MQ=255)
                                                                     aTAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTaa                                                               <  2:298608/149‑1 (MQ=255)
                                                                       aGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGAGTTCCCCCCGCCATCCGCAGCGTGCGGTCATGCGCCAGTTGCTTAACACCAGCCGCCACAGGGTTAAAGGCACGGGGAACg                                                             >  2:112032/1‑149 (MQ=255)
                                                                       aGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACg                                                             <  1:115212/149‑1 (MQ=255)
                                                                            tCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACg                                                        >  2:349256/1‑149 (MQ=255)
                                                                                gctcgctcATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCAGCCGCCAGTTGCTTAACACGAGCCCCCAGACGGCTAACGGCGCGGGTAACGGacccgcac                                                    >  2:410807/1‑142 (MQ=255)
                                                                                  tcgctcATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTc                                                  <  2:204369/149‑1 (MQ=255)
                                                                                     ctcATCATCTCAGGCGCCAGGCCAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGa                                               <  2:190504/149‑1 (MQ=255)
                                                                                       catcatCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAg                                             <  1:193307/149‑1 (MQ=255)
                                                                                          catcTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAgcgc                                          <  1:73118/149‑1 (MQ=255)
                                                                                                       aGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGGTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGGGCCATACTAACGCAg                             >  1:164298/1‑149 (MQ=255)
                                                                                                         gCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAgcg                           <  1:305819/149‑1 (MQ=255)
                                                                                                          cGAATACGCGTCAGCGGCGGGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAgcgg                          <  2:276676/149‑1 (MQ=255)
                                                                                                                   gTCAGCGGCGTGCGCAAGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGGTTAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCCGCGGCGGCGGgat                 >  1:224164/1‑149 (MQ=255)
                                                                                                                                  aaGTCGTGACTTCCCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAAGGCCCCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAAc  >  1:179432/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                        
CAGGTAGTCGATAAACTGCTCAATGATGGCGTTGCACTCTTCGATATCTTTATTGATCGATTCTGCCAGATAGCCATCCTGCTCGCTCATCATCTCAGTCGCCAGGCGAATACGCGTCAGCGGCGTGCGCAAGTCGTGACTTACCCCCGCCATCAGCAGCGTGCGGTCATCCGCCAGTTGCTTAACACCAGCCGCCATATGGTTAAAGGCACGGGTAACGGAACGCACCTCCGAAGCGCCATACTCACGCAGCGGCGGCGGAATAATCCCTTTACCAAC  >  CP009273/3528364‑3528642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑