Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,084,016 Δ2 bp coding (344‑345/900 nt) hisG → ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,084,0160C.98.1% 230.6 / ‑3.5 53coding (344/900 nt)hisGATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (28/24);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
*CP0092732,084,0170C.98.1% 230.9 / ‑3.1 53coding (345/900 nt)hisGATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (28/24);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGC  >  CP009273/2083876‑2084154
                                                                                                                                            ||                                                                                                                                         
ccTTGGGATTATCGGCGAAAATGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCTGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGt                                                                                                                                  <  1:858200/149‑1 (MQ=255)
    gggATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGc                                                                                                                              >  2:48795/1‑149 (MQ=255)
        ttATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCtctc                                                                                                                          <  1:141619/149‑1 (MQ=255)
        tgagcGGCGAAAGCGTGCGGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTCGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCtctc                                                                                                                          <  1:822874/145‑1 (MQ=255)
          aTCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCt                                                                                                                        <  2:816707/149‑1 (MQ=255)
           tCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGccccactt                                                                                                                       >  2:754345/1‑142 (MQ=255)
            cGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTa                                                                                                                      <  2:82813/149‑1 (MQ=255)
            cGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTa                                                                                                                      <  1:235250/149‑1 (MQ=255)
             ggCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTaa                                                                                                                     >  1:1034428/1‑149 (MQ=255)
               cGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCCCCCCTTAAAc                                                                                                                   >  2:1049933/1‑149 (MQ=255)
                gAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACg                                                                                                                  >  1:772851/1‑149 (MQ=255)
                  aaaCGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGCAG‑‑TGGGACGGCTCGCTCTCCTTAAACGGt                                                                                                                >  2:419592/1‑149 (MQ=255)
                    aCGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTaa                                                                                                              <  2:998701/149‑1 (MQ=255)
                         cTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTa                                                                                                         >  1:86637/1‑149 (MQ=255)
                          tGGAAGAAGAGCTGCTTAACCGCCGCGCCGAGGGTGAAGATCCACGCTACTTTGCCCTGCGTCGTCTAGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TTGGACGGTACGCTCTCCTTCAACGGTAAACGTAt                                                                                                        >  1:752236/1‑149 (MQ=255)
                          tGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTAt                                                                                                        >  1:483776/1‑149 (MQ=255)
                            gaagaaGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCg                                                                                                      >  2:1136743/1‑149 (MQ=255)
                             aagaagAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGc                                                                                                     <  1:1046071/149‑1 (MQ=255)
                                 agagCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCAcc                                                                                                 <  1:1209619/149‑1 (MQ=255)
                                     ctgctTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACATATCGCCACCTCtt                                                                                             >  1:773946/1‑149 (MQ=255)
                                       gctTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGCTGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGGAAACGTACCGCCACCCCTTAt                                                                                           >  2:74425/1‑149 (MQ=255)
                                        cttatgcgccgcGCCCAGGGGGAAGCTCCACGCTACTTGACCCTGCGTCGTCTGGATCTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGGATCGCCACCTCTTATc                                                                                          <  2:288148/143‑1 (MQ=255)
                                        ctTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATc                                                                                          <  1:616403/149‑1 (MQ=255)
                                         ttAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATcc                                                                                         >  2:294151/1‑149 (MQ=255)
                                            aCCGCCGCGCCCAGGGTGAAGATCCCCGCTACTTCACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAA‑‑CTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCa                                                                                      <  2:612513/149‑1 (MQ=255)
                                             cctccgcGCCCAGGGTTAAGATCCTCGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTGTCGCTCGCAGCGCCGGTTGATGAAG‑‑TGTGACGGTCCGCTCTCCTTAAACGGTACTCGTATCGCCACCTCTTATCCTCAc                                                                                     <  2:419620/146‑1 (MQ=255)
                                             ccgccccgccCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCAc                                                                                     <  2:810270/149‑1 (MQ=255)
                                               gccgcGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCTCCACCTCTTATCCTCACCt                                                                                   <  1:1231783/149‑1 (MQ=255)
                                               gccgcGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATCAAGC‑‑GGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCt                                                                                   <  1:819882/149‑1 (MQ=255)
                                                   cgcCGAGGGGGAAGTTCCACGCTCCTTTACCCTGCGTCGGCTGGATTTCGGCGGCCGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCGCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCGCACCTGCTc                                                                               <  2:507707/149‑1 (MQ=255)
                                                      ccAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTCTTGCCACCTCTTATCCTCACCTGCTCAAg                                                                            >  2:554666/1‑149 (MQ=255)
                                                          ggTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACTGATCGCCACCTCTTATCCTCACCTGCTCAAGCGtt                                                                        <  2:1092698/149‑1 (MQ=255)
                                                            tGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTCAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTAt                                                                      >  1:285476/1‑149 (MQ=255)
                                                            ggAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCCTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTAt                                                                      <  1:331227/148‑1 (MQ=255)
                                                                gATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCg                                                                  >  2:634696/1‑149 (MQ=255)
                                                                 aTCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTACACGTATCGCCCCCTCTTATCCTCACCTGCTCAAGCGTTATCTCgg                                                                 >  1:642870/1‑148 (MQ=255)
                                                                   ccACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGTcc                                                               >  1:355767/1‑149 (MQ=255)
                                                                    cACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCCCCTGCTCAAGCGGTATCTCGACCa                                                              >  2:364188/1‑149 (MQ=255)
                                                                         tACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAg                                                         <  2:436008/149‑1 (MQ=255)
                                                                                 ccTGCGTCGTCTGGATTGCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATctct                                                 <  2:700013/149‑1 (MQ=255)
                                                                                   cgCGTCGTCTGGACTTCGCCTGCTGTCGCCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCttt                                               <  2:34776/148‑1 (MQ=255)
                                                                                       tcgtcTGGATTTCGGCGGATGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTaaa                                           <  1:9760/149‑1 (MQ=255)
                                                                                           cTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TAGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCCCATCTTCTCAACACCTGCTCAAGCGTTATCTCGACCAGCAAGGCATCTCTTTTCAAACCt                                       >  1:1083122/1‑149 (MQ=255)
                                                                                               aTTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCtt                                   >  2:1131662/1‑149 (MQ=255)
                                                                                                          tGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGtt                        >  2:661029/1‑149 (MQ=255)
                                                                                                          tGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAG‑‑TCGGACGGTCCGCTCTCCTTAAACGGTAAACGTACCGCCACCTCTTATCCTCACCTACTCAAGCGTTATCACAACCCGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGtt                        >  2:439783/1‑149 (MQ=255)
                                                                                                                  tgtccTGGCAACGCCGGGTGAGGAAG‑‑TGGGACGCTCCGCTCTCCTTAACCGGTAACCGTATCGCCACCTCTTACCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGaa                <  2:27400/145‑1 (MQ=255)
                                                                                                                   tCGCTGGCCACGCCGGTTGATGAAG‑‑TGGCACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAg               <  1:322603/149‑1 (MQ=255)
                                                                                                                      cTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCg            >  2:590783/1‑149 (MQ=255)
                                                                                                                      cTGGCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGGCCTGTTGAAGTCg            >  2:80708/1‑149 (MQ=255)
                                                                                                                         gCAACGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGccc         >  1:183829/1‑149 (MQ=255)
                                                                                                                           aaCGCCGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGACCGGTTCTGTTGAAGTCGCCCcg       >  1:861407/1‑149 (MQ=255)
                                                                                                                                cGGTTGATGAAG‑‑TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGc  >  1:477268/1‑149 (MQ=255)
                                                                                                                                            ||                                                                                                                                         
CCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGGATTTCGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGC  >  CP009273/2083876‑2084154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: