Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,305,991	G→T	Q80K (CAG→AAG)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,305,991	0	G	T	100.0%	216.8 / NA	63	Q80K (CAG→AAG)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/28);  total (35/28)

ACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAT  >  CP009273/2305849‑2306136                                                                                                                                               |                                                                                                                                                  aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGTTATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGGTGGTTTCGTTTTGAGCGCTGTTGCCCTGGATCTTatatgc                                                                                                                                             >  2:171175/1‑147 (MQ=255)  cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCTAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTcc                                                                                                                                            <  2:147866/149‑1 (MQ=255)  cGTCGGGCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTcc                                                                                                                                            <  2:147892/149‑1 (MQ=255)   gTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACAAGGCTCCAGGGCTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTCTATTccc                                                                                                                                           >  2:34234/1‑149 (MQ=255)     cGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCAc                                                                                                                                         >  1:512247/1‑149 (MQ=255)          cAGGAAGTTACGACATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGcc                                                                                                                                    <  2:177376/149‑1 (MQ=255)            ggAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGt                                                                                                                                  >  1:114080/1‑149 (MQ=255)              aaGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTaa                                                                                                                                <  2:115934/149‑1 (MQ=255)                 ttACGTCATAAAGCACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCg                                                                                                                             <  1:71091/149‑1 (MQ=255)                 ttACGTCATAAACAATCCAGTAGTTACGACCGTAGACGAAATAACCCACATCCTTTAAGTTCAGCCCTTGTAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCATCGCTGTGGCGCTGGATCTTATATTCCCGCTGCCCGTAACCg                                                                                                                             <  1:109116/149‑1 (MQ=255)                 ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCATATATTCCCACTGGCTGTAACCg                                                                                                                             <  1:833491/149‑1 (MQ=255)                 ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCATTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCg                                                                                                                             >  2:479119/1‑149 (MQ=255)                       caaacacaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGc                                                                                                                       <  1:970490/144‑1 (MQ=255)                         tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTg                                                                                                                     >  2:271730/1‑149 (MQ=255)                         tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTg                                                                                                                     >  2:815789/1‑149 (MQ=255)                           accaacGTCGTCGTTACGACCGCAGTCGCAAGAACCCACCGCCTGGAGTTTCAGACCTGCGCATGCCATACGGGTCCAGGAGTTGTTTTCGTTTTCATCGCTGCCGCCCTGGATGTTATATTCCCACTGGCCGTAACCGGTCAGCTGGt                                                                                                                   <  1:405802/147‑1 (MQ=255)                           aacaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGt                                                                                                                   <  1:424951/149‑1 (MQ=255)                              aacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGCTCTTATATTCCCTCTGGCCGTAACAGGTCAGCTGGTCAg                                                                                                                >  2:1217467/1‑149 (MQ=255)                               acGCCGTAGTTACGACCGTAGTCGAAAGACCCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGt                                                                                                               <  1:705515/149‑1 (MQ=255)                                 gCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCCCTGGCCGTAACCGGTCAGCTGGTCAGtta                                                                                                             >  1:545810/1‑147 (MQ=255)                                        ttACGACCGTAGTCGAAAGAACCCACATCCTGGACTTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTACCCTGAGt                                                                                                      >  1:682412/1‑149 (MQ=255)                                             acggtagtaGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc                                                                                                 <  2:249470/146‑1 (MQ=255)                                             aCCGTAGTCGAAAGAACCCACATCCGGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc                                                                                                 <  1:1114881/149‑1 (MQ=255)                                             aCCGTAGGCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCAc                                                                                                 <  2:1120758/149‑1 (MQ=255)                                               cGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCt                                                                                               >  2:920327/1‑149 (MQ=255)                                                      gAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGc                                                                                        >  2:305518/1‑149 (MQ=255)                                                      gAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGc                                                                                        <  2:42236/149‑1 (MQ=255)                                                       aaaGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATCTTCCCGGGGGCTTTTACCGGTCAGCTGGTCAGTATGCTGAGTTTCACCTTTGACGcc                                                                                       >  2:581346/1‑149 (MQ=255)                                                          gAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAg                                                                                    <  2:503692/149‑1 (MQ=255)                                                             cccACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGGTCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTCACCTGAGTTTCACCTTTGAAGCCAAGACg                                                                                 >  2:1069584/1‑149 (MQ=255)                                                                  aTCCTGGAATTTCAGGCCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGt                                                                            <  2:84287/149‑1 (MQ=255)                                                                   tCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGCGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCTCTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGGGTTTCACCTTTGAAGCCAAGACGCATGTa                                                                           <  2:859830/149‑1 (MQ=255)                                                                      tGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGtcagt                                                                        >  1:683561/1‑145 (MQ=255)                                                                      tGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGt                                                                        >  2:217369/1‑149 (MQ=255)                                                                              cAGTCCTGCGAATGCCACTCGGCTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGc                                                                <  2:112586/149‑1 (MQ=255)                                                                                 aCCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCAt                                                             >  1:705897/1‑149 (MQ=255)                                                                                      cGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCACGACGCATGTAGGTCTGGTCGCCATCTACa                                                        >  1:851280/1‑149 (MQ=255)                                                                                           gCCACACGGGTCCAGGAGTTGTGTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCttt                                                   <  1:51872/149‑1 (MQ=255)                                                                                             cacgCGGGTCGAGGAGTTGTTTTCGTTTTCAGCGCTGTGGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgt                                                 <  1:34234/149‑1 (MQ=255)                                                                                             cacaCGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgt                                                 >  2:1006482/1‑149 (MQ=255)                                                                                              acacGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgtt                                                <  2:920417/149‑1 (MQ=255)                                                                                              acacGGGCCCAGGGGTTGTTTGCTGTTTCAGCGCTGTTGCACTGTGTGTTTGGTTCCCACTGGCCGGAACCGGTCATCTGGTCAGTAGCCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgtt                                                <  1:44986/149‑1 (MQ=255)                                                                                                acGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTtgttgt                                              >  1:119065/1‑149 (MQ=255)                                                                                                 cGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTc                                             <  2:512247/149‑1 (MQ=255)                                                                                                        aGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGGGaaa                                      >  1:431076/1‑149 (MQ=255)                                                                                                               gttttcgttttcAGCGCTGTTGCCCTGGATCTTATATTCACACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTCCATCTTTGTTGTCAGAGAAATAGTGcg                               >  1:222105/1‑148 (MQ=255)                                                                                                               gttttcgttttcAGCGCTGGTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCGCCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCa                               <  2:603705/149‑1 (MQ=255)                                                                                                                 tttcgttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTGCATCTTTGTTGTCAGAGAAATAGTGCAgg                             >  1:602757/1‑149 (MQ=255)                                                                                                                  ttcgttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGGTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGc                            >  2:358269/1‑149 (MQ=255)                                                                                                                    cgttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTCACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACCTCTTTGTTGTCAGAGACATAGTGCAGGCCg                          >  2:203524/1‑149 (MQ=255)                                                                                                                     gttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCCTCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                         >  1:167269/1‑149 (MQ=255)                                                                                                                     gttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgttg                                               >  2:977045/1‑127 (MQ=255)                                                                                                                     gttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTttgttg                                               <  1:977045/127‑1 (MQ=255)                                                                                                                     gttttcAGCGCTGTTGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                         >  2:772425/1‑149 (MQ=255)                                                                                                                                  ttGCCCTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTa            <  2:154988/149‑1 (MQ=255)                                                                                                                                     cccTGGATCTTATATTCCCACGGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGGGAAATAGTGCAGGCCGTCTACTTTACCGTACAg         >  1:719140/1‑149 (MQ=255)                                                                                                                                       cTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGag       >  2:1178087/1‑148 (MQ=255)                                                                                                                                       cTGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGAt       >  2:339718/1‑149 (MQ=255)                                                                                                                                        tGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc      >  1:476181/1‑149 (MQ=255)                                                                                                                                        tGGATCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc      >  1:99953/1‑149 (MQ=255)                                                                                                                                           aTCTTATATTCCCGCTGGCCGTAACCGGTCAGGTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTaa   <  1:479119/149‑1 (MQ=255)                                                                                                                                            tCTTATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTact  >  2:382769/1‑147 (MQ=255)                                                                                                                                            tCTTATATTCCCACTGGCCGTAACCGAACACTTCGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAt  >  2:1208844/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                  ACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGTTTTCAGCGCTGTTGCCCTGGATCTGATATTCCCACTGGCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAAT  >  CP009273/2305849‑2306136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑