Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	444,779	C→T	W440* (TGG→TAG)	cyoB ←	cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	444,779	0	C	T	100.0%	226.7 / NA	77	W440* (TGG→TAG)	cyoB	cytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/38);  total (39/38)

CAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGC  >  CP009273/444636‑444917                                                                                                                                                |                                                                                                                                           cAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGTCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAg                                                                                                                                       <  2:515820/149‑1 (MQ=255)  aacctgctgGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGt                                                                                                                                      <  1:409605/146‑1 (MQ=255)  aaTGTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCACAAAGGGAACGAAGAAGGCGATGATCCAGGACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGt                                                                                                                                      <  1:285884/149‑1 (MQ=255)           gCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGGTTAACCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGGCATcc                                                                                                                             >  1:956704/1‑149 (MQ=255)           gCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGAGCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAATAGGTCATcc                                                                                                                             >  2:338487/1‑149 (MQ=255)             tCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCt                                                                                                                           >  1:1101820/1‑149 (MQ=255)             tCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCt                                                                                                                           >  2:753795/1‑149 (MQ=255)               aaaCGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGc                                                                                                                         >  2:131352/1‑149 (MQ=255)               aaaCGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTGTCGTTCAGTTTGAAACCGAAAGCTTTAGGCTACCAGTAGGTAATCACTGc                                                                                                                         >  2:353748/1‑149 (MQ=255)                aaCGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATACAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGGTTGAAACCGAACGCTTTAGGCTGCCAGTAGGTCATCCCTGca                                                                                                                        >  1:647280/1‑148 (MQ=255)                  cgacgGGTCCTGCCCATGCAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCTTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGaa                                                                                                                      <  1:117807/149‑1 (MQ=255)                   ggcgGGGCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAag                                                                                                                     <  2:361714/147‑1 (MQ=255)                   gacgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAag                                                                                                                     <  2:872071/149‑1 (MQ=255)                      cggTCATGCCCAGTAAGCCCAGCGCACACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCTTTCAGTTTCAAACCCAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAagcag                                                                                                                  <  2:65948/148‑1 (MQ=255)                          cATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGa                                                                                                              <  2:1139904/149‑1 (MQ=255)                           aTGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGaa                                                                                                             >  2:135349/1‑149 (MQ=255)                            tGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGCAg                                                                                                            >  2:397782/1‑149 (MQ=255)                             gCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGGACGCGTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGa                                                                                                           >  2:532680/1‑149 (MQ=255)                             gCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGa                                                                                                           <  2:271690/149‑1 (MQ=255)                             gCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGa                                                                                                           <  2:1038974/149‑1 (MQ=255)                              cccgggAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGAc                                                                                                          <  2:362457/144‑1 (MQ=255)                              cccATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGAc                                                                                                          >  1:530918/1‑149 (MQ=255)                              cccATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAAAAGACAATCGCGCGTTTGAAACTGTGTTCGTTCAGTTTGAAACCGAACGCATTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGAc                                                                                                          >  2:591696/1‑149 (MQ=255)                                   gAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAcgc                                                                                                     >  2:382832/1‑149 (MQ=255)                                   gAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAcgc                                                                                                     >  2:382191/1‑149 (MQ=255)                                    aaGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTTGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAcgcc                                                                                                    >  2:830845/1‑149 (MQ=255)                                        ccAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCACTAGGTCATCCCTGCGACGCAGCCAAAGACCACGcccccg                                                                                                >  1:28351/1‑149 (MQ=255)                                         cAGCGCATACAGTGGCATAAACGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAATACCACGCCGCCga                                                                                               >  2:874355/1‑149 (MQ=255)                                           gcgcAAACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatg                                                                                             <  1:1120556/149‑1 (MQ=255)                                                  cagggtcATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGGTTACCCCAGGTATCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGt                                                                                      <  1:705766/143‑1 (MQ=255)                                                    gtcgCAAAAAGGCAACGAAGAAGCCGACGATCCAGGAACAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTa                                                                                    <  1:1025833/146‑1 (MQ=255)                                                         aTAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGaa                                                                               >  2:702293/1‑149 (MQ=255)                                                            aaGGCAACGAAGAACCCGAGGAACCAGAACCAGAACGCGCGTTGACCCCAGGTTACGTTCAGTTTGAAACCGAACGCATTAGGCTACCAGTAGGCCAACCCTGCGAAGCAGCCGAAGACCCCGACGCCAATGAGCACGATATGGAAGta                                                                            >  1:705779/1‑148 (MQ=255)                                                             aGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAc                                                                                                       <  2:379295/121‑1 (MQ=255)                                                             aGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAc                                                                                                       >  1:379295/1‑121 (MQ=255)                                                              ggCAACGAAGAAGCCGATGATCCAGAACCAGACCGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCTGATGATCACGTTATGGAAGTgcg                                                                          <  2:599284/149‑1 (MQ=255)                                                              ggCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCGGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTgcg                                                                          <  2:599236/149‑1 (MQ=255)                                                               gCAACGAAGAAGCCGATGATCCAGAAACCGCTCGAACTGTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGCAgcgcgc                                                                         >  1:868788/1‑149 (MQ=255)                                                                 aaCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCGCGTTATGGAAGTGCGCaa                                                                       >  2:217572/1‑149 (MQ=255)                                                                 aaCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCaa                                                                       >  1:970364/1‑149 (MQ=255)                                                                    gaagaaGCCGATGATCCAGAACCAGAACGCGCGGTTACACCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGATCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCa                                                                    >  1:809651/1‑149 (MQ=255)                                                                    gaagaaCCCGATGATCCAGAACCAGAACGCGGGTTTCCCCCAGGTCTCGTTCAGTTTTAAACCGAACGATTTAGGCTACCAGTAGGTCATCGCTGCGAAGAAGCCGAAGACAACGCCGCCGATGATCCAGTCATGGAAGTgcgcgctcc                                                                    >  1:583186/1‑144 (MQ=255)                                                                     aagaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAg                                                                   >  2:115490/1‑149 (MQ=255)                                                                      agaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg                                                                  <  2:724088/149‑1 (MQ=255)                                                                      agaagCCGATGATCCAGAACCAGAACGCGCCTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGACCAGATAATGGAATTACGCAAACAgg                                                                  >  1:840825/1‑149 (MQ=255)                                                                        aagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGAAGATCACCTTATGGAAGTGCGCACTCAGGaa                                                                >  2:336604/1‑149 (MQ=255)                                                                          gCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACa                                                              <  2:1101820/149‑1 (MQ=255)                                                                                  tCCAGAAGCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGCCATGGAAGTGCGCAATCAGGACCAGGcagcga                                                      >  1:147350/1‑144 (MQ=255)                                                                                  tCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGCGCGCAAGCAGGAACAGGCAGtgc                                                      >  1:663263/1‑147 (MQ=255)                                                                                    cagaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATg                                                    >  2:442415/1‑149 (MQ=255)                                                                                     agaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGc                                                   >  1:614776/1‑149 (MQ=255)                                                                                      gaaccagaccGCGCGTTTCCCCCAGTTTTCGTTCAGTTTGAACCCGAACGCTTTCGTCTACCTGTAGGTCATCCCTGCGAAGCAGCCGAAGCCCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCa                                                  <  1:989122/149‑1 (MQ=255)                                                                                         ccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGaa                                               <  2:487923/149‑1 (MQ=255)                                                                                         ccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTATGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAACCAGCAACAGGCTGTTCTGCAGaa                                               <  1:830845/149‑1 (MQ=255)                                                                                          cgggacGCGCGTTTACCCCAGGTTTCGGTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTGATGCAGAAc                                              <  1:653611/145‑1 (MQ=255)                                                                                          cagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAAc                                              <  1:115490/149‑1 (MQ=255)                                                                                             cacGCGCCTCTACCCCCGGTTTAGTTCAGTTTTAAACCGAACGCTGTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGaa                                           <  1:516739/148‑1 (MQ=255)                                                                                              tcggcggTTTACCCCAGGTTTCGTTCAGTTTGAAAGCGGACGCTTTGGGCTACCAGTAGGTCATCCCTGCGGAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAg                                          <  1:670911/143‑1 (MQ=255)                                                                                               cgcgcgTTTACCCCAGGTTTCGTTCCGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCTGCCCTGCGAAGCAGCCGAAGACCACGCCCCCGATGATCACGCTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACCAAGt                                         >  1:748575/1‑149 (MQ=255)                                                                                                gcgcgTTTACCCCGGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTc                                        <  1:29244/149‑1 (MQ=255)                                                                                                gcgcgTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTc                                        >  1:1159762/1‑149 (MQ=255)                                                                                                 cgcgTTTCCCCTCGGTTTCGTTCAGTTTGAAAGCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGTCCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCATGAACAGGCTGTTATGCAGAACGAAGTcc                                       <  1:687919/149‑1 (MQ=255)                                                                                                      ttACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTAGTTCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAGCGAAGTCCGCGcc                                  <  2:835032/149‑1 (MQ=255)                                                                                                        aCCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCg                                <  1:753795/149‑1 (MQ=255)                                                                                                          tccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGTCTGTCATGCAGAACGAAGTCCGCGCCCGGt                              <  2:632898/148‑1 (MQ=255)                                                                                                          cccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATGAGGAACAGGCTGTTATGCAGAACGAAGTCAGCGCACgtt                              >  1:1094598/1‑147 (MQ=255)                                                                                                          cccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGt                              >  2:1215935/1‑149 (MQ=255)                                                                                                                tttCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGcc                        <  2:721907/149‑1 (MQ=255)                                                                                                                tttCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGcc                        <  2:1061300/149‑1 (MQ=255)                                                                                                                  tCGGTCAGGCTGAAACCGAACGCTGTAGGCTACCAGTAGGTCATCCCTGCCAATCAGCCGAAGCCCACGCCGCCGATGATCACGTTATGGAAGGGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcag                      <  2:96127/149‑1 (MQ=255)                                                                                                                       cAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAgcagca                 >  2:1168547/1‑149 (MQ=255)                                                                                                                       cAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCAGCCCTGCGAAGCAGACGAAGAAAACGACGCCGAAGCTCACGTTAAGGAAGTGCGCAATCAGGAACAGGCAGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAgcagca                 <  1:244388/149‑1 (MQ=255)                                                                                                                            tGAACCCGAACGCTTTAGGCTACCCGTAGGTCATCCCTCGAAAGTGGCCGAAGGCCGCGCCGCCGATGATAATGTTATGGAAGTTCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCa            <  1:591696/149‑1 (MQ=255)                                                                                                                            tGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCa            <  2:100811/149‑1 (MQ=255)                                                                                                                                 ccGAACGCTTTAGGCTACCAGTAGGTCGTAGAGGCTAAGCATGCGAAGACTACGCCGCCGATGATCACGTTATGGAAGTGCGCCATCAGGAACAGGCTGTAATGCAGAACGAAGTCAGAGCCCGTTACGGCAAGCGGCAGGcccagcac       >  1:1174719/1‑143 (MQ=255)                                                                                                                                   gAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCGGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATcc     >  1:1087645/1‑149 (MQ=255)                                                                                                                                      cGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTAGGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGc  <  2:840825/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                           CAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGC  >  CP009273/444636‑444917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑