Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	982,560	T→A	intergenic (‑122/+481)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	982,560	0	T	A	100.0%	218.8 / NA	67	intergenic (‑122/+481)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (37/30);  total (37/30)

CTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACA  >  CP009273/982414‑982705                                                                                                                                                   |                                                                                                                                                  ccgcaagcatatTCCGCTTCCTCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGGCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTAcc                                                                                                                                                 <  1:1281289/141‑1 (MQ=255)   gCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGATAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAt                                                                                                                                               >  2:210842/1‑149 (MQ=255)    ccAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt                                                                                                                                              >  2:1077936/1‑149 (MQ=255)    ccAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCAtt                                                                                                                                              >  2:689265/1‑149 (MQ=255)        aaTATTGCGGATCATCATTATTTATTACCATCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTAGTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCaaa                                                                                                                                          <  2:886614/149‑1 (MQ=255)            ttGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTaa                                                                                                                                      >  1:1154627/1‑149 (MQ=255)                         ttatttatTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCg                                                                                                                         >  1:674187/1‑149 (MQ=255)                           atttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTTTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTg                                                                                                                       >  1:1306890/1‑149 (MQ=255)                                   cccACATGGTTTTTTTTATGACCCCGGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGGACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCt                                                                                                               <  1:37537/149‑1 (MQ=255)                                        aTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaa                                                                                                          <  1:1356824/149‑1 (MQ=255)                                           gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATTTGTCTGCAGGCATCTTACCCTTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat                                                                                                       >  2:374569/1‑149 (MQ=255)                                           gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat                                                                                                       <  1:1003896/149‑1 (MQ=255)                                           gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat                                                                                                       >  1:498518/1‑149 (MQ=255)                                             tttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa                                                                                                     <  2:1129874/149‑1 (MQ=255)                                                 tttATGACACCTGCCACTGCCGTCACTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat                                                                                                 >  1:833579/1‑149 (MQ=255)                                                 tttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAATTGCTACaaagataaagat                                                                                                 >  1:50249/1‑149 (MQ=255)                                                 tttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTCCaaagataaaggt                                                                                                 >  1:745522/1‑147 (MQ=255)                                                  ttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAtt                                                                                                <  1:830990/149‑1 (MQ=255)                                                   tATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAttt                                                                                               >  1:614922/1‑149 (MQ=255)                                                    ttggccccTGCCGCTGCGGTCAATAAGTTCTGTCAATTAAAATTTACGGAACTATTGATGGGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTg                                                                                              <  2:134047/143‑1 (MQ=255)                                                     tGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCATGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgg                                                                                             <  2:502110/149‑1 (MQ=255)                                                     tGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACCATTGATGAGATTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgg                                                                                             <  2:1046875/149‑1 (MQ=255)                                                        cacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGttt                                                                                          <  1:154864/149‑1 (MQ=255)                                                        cacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGttt                                                                                          <  2:1170959/149‑1 (MQ=255)                                                           cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                       >  1:498231/1‑149 (MQ=255)                                                           cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                       >  1:505184/1‑149 (MQ=255)                                                           cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                       >  1:505580/1‑149 (MQ=255)                                                           cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTAACGCAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGCTAAAAATTTGGTTTCaa                                                                                       >  2:584869/1‑149 (MQ=255)                                                                 cttccGTTAATAAGTTCTGTCAATAAAAATTTTCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaa                                                                                 <  2:629569/146‑1 (MQ=255)                                                                     cGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaaa                                                                             >  1:717994/1‑149 (MQ=255)                                                                     cGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaaa                                                                             >  1:966472/1‑149 (MQ=255)                                                                      gTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAat                                                                            <  1:689265/149‑1 (MQ=255)                                                                        cAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAatat                                                                          <  1:1077936/149‑1 (MQ=255)                                                                          aTAAGTTCGGTCAATAAAAATTTACGGAACTATTGATGAGAGAATGAAATAAATATGTGTCGGCAGGAAACTTACCATTCAAACAACCGTTTCGCTACAGTGAAAGTGCTAAAAAGATAAAGATTTGGAAAAAAAAAAAAAAAATATGt                                                                        <  2:392861/149‑1 (MQ=255)                                                                          aTAAGGTCTGTAAATAAAAATTTGCGGAACTATTGTTGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTAACATTCAAACTAACGTTTCGCTACCGCGAAAGTGCTACAAAGATAATGATTTGGTTTCAAAAAGAAAAAATATGt                                                                        <  2:635701/149‑1 (MQ=255)                                                                               ttCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCa                                                                   >  1:760327/1‑149 (MQ=255)                                                                                tccgTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaa                                                                  <  1:1278572/146‑1 (MQ=255)                                                                                  tggcAATAAAAATTTACGGGACTATTGATGATAGATTGGTGTTTTTATGTGTCTGCAAGCAACTGACCAATCAAAATAACGTTACGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaaaa                                                                <  2:815723/146‑1 (MQ=255)                                                                                       aTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaa                                                           >  1:567704/1‑149 (MQ=255)                                                                                        tAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaaa                                                          >  1:713199/1‑149 (MQ=255)                                                                                        tAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCACTGTTGGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaaa                                                          >  1:681637/1‑149 (MQ=255)                                                                                             aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                     <  1:892954/149‑1 (MQ=255)                                                                                             aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                     >  2:95739/1‑149 (MQ=255)                                                                                              tttACTGAACTATTAATAAGAGTTTAGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAATGCTACAAAGAAAAAAATTAAGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAg                                                    <  2:7151/149‑1 (MQ=255)                                                                                                   ggAACTATTGATGAGAGTTTGGTGTCTTTATCAAACTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                               >  1:1030679/1‑149 (MQ=255)                                                                                                     aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGCACttt                                             >  2:613123/1‑149 (MQ=255)                                                                                                        tATTGATGCGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg                                          <  2:1361526/149‑1 (MQ=255)                                                                                                        tATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg                                          <  2:206152/149‑1 (MQ=255)                                                                                                            gatgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAGGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAaca                                      >  2:802322/1‑149 (MQ=255)                                                                                                              tgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAcc                                    >  1:994423/1‑149 (MQ=255)                                                                                                              tgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAAAAcc                                    >  1:21387/1‑149 (MQ=255)                                                                                                                agagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACTAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAGCTTTGTGAACACAGt                                  >  2:1227316/1‑149 (MQ=255)                                                                                                                          gtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa                        >  1:298078/1‑149 (MQ=255)                                                                                                                              ttATGTGTCTGCCGGCATATGACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATaga                    <  1:27453/149‑1 (MQ=255)                                                                                                                                aTGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAtcgtga                  >  2:290215/1‑144 (MQ=255)                                                                                                                                 tgtgTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAACATTATGTAACCAAAAGTAAAATTTAAGAAACTTGGTGAGCACCGTCATATTTGCAGAGATAc                 >  2:1316178/1‑149 (MQ=255)                                                                                                                                  gcgccTGCAGCCACCTTACCATTCACACTAACGTTCGGCTACGCAGAAAGTGATACAGAGATAAAGATCTGGTTTCAAAAAGAAAAAATATGTAACCTAAAGTAGAAGTTAAGGAACTCTGTGAACACCGTCATATTTCCATAGAGACg                <  2:959465/145‑1 (MQ=255)                                                                                                                                    gtCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAATGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtg              >  1:843490/1‑149 (MQ=255)                                                                                                                                       tGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatg           >  1:1318865/1‑149 (MQ=255)                                                                                                                                          aGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGata        >  1:347132/1‑149 (MQ=255)                                                                                                                                          aGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGata        >  2:598012/1‑149 (MQ=255)                                                                                                                                            gCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt      <  2:717994/149‑1 (MQ=255)                                                                                                                                            gCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt      <  2:505580/149‑1 (MQ=255)                                                                                                                                            gCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt      <  2:505184/149‑1 (MQ=255)                                                                                                                                            gCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt      <  2:966472/149‑1 (MQ=255)                                                                                                                                              aTCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTa    <  1:1240181/149‑1 (MQ=255)                                                                                                                                                cTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAGGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACa  >  2:1300139/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACA  >  CP009273/982414‑982705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑