Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 630,174 C→T A378V (GCA→GTA)  ybdL → methionine aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273630,1740CT98.8% 274.8 / NA 84A378V (GCA→GTA) ybdLmethionine aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (48/35);  minor base G (1/0);  total (49/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTT  >  CP009273/630031‑630318
                                                                                                                                               |                                                                                                                                                
tACCCTTGATGTTGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGTGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGACAAGAAGGAATCGACGTTGCTGGtagcag                                                                                                                                             <  2:12346/149‑1 (MQ=255)
tACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGtagcag                                                                                                                                             >  2:1034503/1‑149 (MQ=255)
tACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGtagcag                                                                                                                                             >  2:1115781/1‑149 (MQ=255)
tACCCTGGATGATGTTGAGTTTTGCCAGTGGCGGACGCAGGCGCCCGGAGTGACTTCTATTCCGCTGGCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGtagcag                                                                                                                                             <  1:267345/149‑1 (MQ=255)
   ccTGGATGATGTTGAGTTTTGCCAGGGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTg                                                                                                                                          <  1:448542/149‑1 (MQ=255)
     catttttagtggAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGaa                                                                                                                                        >  2:1214934/12‑149 (MQ=255)
          gatgTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACATTGCGGGGAGCAGCTGAACGCCt                                                                                                                                   >  2:978127/1‑149 (MQ=255)
              ttGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTgagc                                                                                                                               >  2:990669/1‑149 (MQ=255)
                  gTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGc                                                                                                                           >  1:833031/1‑149 (MQ=255)
                   ttttGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCt                                                                                                                          >  2:891234/1‑149 (MQ=255)
                   ttttGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCGTCCCACATAAACTGATTCGTCTCTGTGTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCt                                                                                                                          >  2:774923/1‑149 (MQ=255)
                    tttGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCtt                                                                                                                         <  1:291833/149‑1 (MQ=255)
                      tGCCTGTGGCGGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATACCCCGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTa                                                                                                                       <  1:1060910/149‑1 (MQ=255)
                      tGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGCATCGACGTTGCTGGTAGCAGCTGAACGCCCGCGCCAGCTTTa                                                                                                                       >  2:226904/1‑149 (MQ=255)
                        ccAGTGGCGGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGGGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCACCTTTAGc                                                                                                                     >  1:201692/1‑149 (MQ=255)
                         cAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCGGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCt                                                                                                                    >  2:313605/1‑149 (MQ=255)
                         cAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCATTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGGATCGACGTTGCTGGTAGGAGCTGAACGCCTGCGCCAGCTTTGGCt                                                                                                                    >  1:1161364/1‑149 (MQ=255)
                          aGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGGGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGCCTCTGTATTGTCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTa                                                                                                                   <  1:46188/149‑1 (MQ=255)
                            tGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTAtt                                                                                                                 <  1:1147314/149‑1 (MQ=255)
                            tGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTAtt                                                                                                                 >  1:248461/1‑149 (MQ=255)
                              gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTa                                                                                                               >  1:1085291/1‑149 (MQ=255)
                              gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTa                                                                                                               <  1:492382/149‑1 (MQ=255)
                              gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCCAtttt                                                                                                               >  2:212380/1‑148 (MQ=255)
                              gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGACGATCCCTTCCAACATAAAATGATTCGTCGCTGTTTTTCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGGCTGCGCCAGCTTTAGCTATTTa                                                                                                               >  1:376419/1‑149 (MQ=255)
                              gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTACGCCGATCCCTACCCACATAAACTGGTTCGGCTCTGTTTTGCCTAGAAGGAATCTACGGTGCGTGTAGCAGCTGAACGCCTCCGCCAGCTTTAGCTATTTa                                                                                                               >  2:1025474/1‑149 (MQ=255)
                              gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGGGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGCACGCCTGCGCCAGCTTTAGCTATTTa                                                                                                               >  1:1338797/1‑149 (MQ=255)
                                tGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAAc                                                                                                             >  1:1128433/1‑149 (MQ=255)
                                 gACGCAGGTGCAGGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATACACTGATGCGTCTCTGTTGTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTATcc                                                                                                            <  2:800793/149‑1 (MQ=255)
                                    gCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGCTTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGTTAGCAGCTGCACGCCTGCGCCCGCTTTAGCTATTTGACGGTc                                                                                                         >  2:540268/1‑149 (MQ=255)
                                    gCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTGTTTAACCGTc                                                                                                         >  1:1276679/1‑149 (MQ=255)
                                    gCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTc                                                                                                         >  1:757926/1‑149 (MQ=255)
                                    gCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACATTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTc                                                                                                         >  2:955299/1‑149 (MQ=255)
                                          gCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGGCCAGgcg                                                                                                   >  1:713924/1‑149 (MQ=255)
                                          gCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCCATTTAACCGTCCAGgcg                                                                                                   >  1:1111843/1‑149 (MQ=255)
                                           cACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTGTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCAGGgcgg                                                                                                  >  1:155085/1‑148 (MQ=255)
                                            aCGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGcgcg                                                                                                 <  1:1286671/149‑1 (MQ=255)
                                               gCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGa                                                                                              <  2:1357487/149‑1 (MQ=255)
                                                   aGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAatat                                                                                          <  1:1350637/149‑1 (MQ=255)
                                                   aGCGGCGATTCCGCTGTCGGTGTGTTGCGCCGACCCCTTCCCACATAAACGGATTCGTCTCTGTTTTGCCAAGAAGGAATCGAAGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAatat                                                                                          <  1:471536/149‑1 (MQ=255)
                                                    gcggcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATAtt                                                                                         <  2:5165/149‑1 (MQ=255)
                                                    gcggcgATTCCGCGGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATAtt                                                                                         <  2:392754/149‑1 (MQ=255)
                                                      ggctATTCCGCCGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGa                                                                                       <  2:36361/149‑1 (MQ=255)
                                                      ggcgGTTCCGGTGTCGGTGTTGTGTGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGATCGCTGCAGCCATTTAACCGTCCAGGCGCGGGAATATTGa                                                                                       <  2:303435/149‑1 (MQ=255)
                                                       gcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGAc                                                                                      <  1:531842/149‑1 (MQ=255)
                                                       gcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGAc                                                                                      >  1:126673/1‑149 (MQ=255)
                                                            tCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGCACGCCTGCGCCAGCTTTAGCTGTTTAACCTTCCAGGGGCGCGCATATTGACGGTCg                                                                                 >  1:292173/1‑149 (MQ=255)
                                                            tCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGGCGGCCg                                                                                 <  2:1064806/149‑1 (MQ=255)
                                                            tCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCg                                                                                 >  2:713431/1‑149 (MQ=255)
                                                               gCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCa                                                                              >  1:1122199/1‑149 (MQ=255)
                                                               gCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCa                                                                              >  2:1193333/1‑149 (MQ=255)
                                                                cTGGCGGTGTTTTGCGCCGATGCCTTCCCACAAAAACTGCTTCGACTCTGTGTTGCCAAGAAGGACTCGCCGTTTCGGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGCCCAGGCGCGGGAATATTGACGGTCGGCaa                                                                             <  2:1195200/149‑1 (MQ=255)
                                                                     ggTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaacaac                                                                        >  1:1100162/1‑149 (MQ=255)
                                                                         ttttGCGCCGATCACTTCCCACATAAACTGATTCGTCTCTGCTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCTGCAAACAACTCtt                                                                    >  1:1002491/1‑149 (MQ=255)
                                                                          tttGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAACCAACTCTTg                                                                   >  2:1144836/1‑149 (MQ=255)
                                                                          tttGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTg                                                                   <  2:794734/149‑1 (MQ=255)
                                                                          tttGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTg                                                                   <  1:1115781/149‑1 (MQ=255)
                                                                              cgcCGACCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAgg                                                               <  2:413842/149‑1 (MQ=255)
                                                                                ccGTTCCCTTCCCACATAAACTGATTCGTTTCTGTTTGGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGcc                                                             <  2:1232860/149‑1 (MQ=255)
                                                                                 cGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCg                                                            >  1:1008358/1‑149 (MQ=255)
                                                                                       cTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAAtt                                                      >  2:1148492/1‑149 (MQ=255)
                                                                                        ttCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACGCTTGCAGGCCGTTAAttt                                                     >  2:935517/1‑149 (MQ=255)
                                                                                         gcccACATAGACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTTGTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTAGGCAAACAACTCTTGCAGGCCGTTAATTTg                                                    <  2:958921/148‑1 (MQ=255)
                                                                                          cccACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGc                                                   <  1:1261070/149‑1 (MQ=255)
                                                                                            cacaTAAACTGATTCGTCTCTGTTTTGCCAAGAAGGACTCGACGTTGCTTGTAGCAGATGAACGCCTGCGCAAGCTTTAGCTATTTAACCGTCCAGGCGGGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCtt                                                 >  2:1050639/1‑149 (MQ=255)
                                                                                                tAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAgg                                             <  1:571605/149‑1 (MQ=255)
                                                                                                  aaCTGCTTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGgcg                                           <  1:420985/149‑1 (MQ=255)
                                                                                                  aaCTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGgcg                                           >  1:1358576/1‑149 (MQ=255)
                                                                                                     tGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCgcag                                        <  1:1253969/149‑1 (MQ=255)
                                                                                                     tGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCCGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCGAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCgcag                                        >  2:497393/1‑149 (MQ=255)
                                                                                                     tGATTCGGCTCTGTTTGGCCAAGAAGGAATCGACGTGTCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCgcag                                        <  2:1114145/149‑1 (MQ=255)
                                                                                                      gATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCgcagc                                       <  2:879279/149‑1 (MQ=255)
                                                                                                       aTTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCgcagca                                      >  2:1074540/1‑149 (MQ=255)
                                                                                                            tctcTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTtcg                                 >  2:1263092/1‑149 (MQ=255)
                                                                                                            tctcTGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGAAGCAGtttg                                 >  2:215833/1‑147 (MQ=255)
                                                                                                              tctGTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCCGCCTTAGCTCTTTAACCGTCCAGCCGCGGGAATATTGACGGTGGGCAAACAGCTCTGGGAGGCCGGTAAGTTGCTTCCTGCGCCGCACTtcgtc                               >  2:471397/1‑149 (MQ=255)
                                                                                                                 gTTTTGCCAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCt                            >  2:1281317/1‑149 (MQ=255)
                                                                                                                  gtttGCCAAGAAGGAATCGACGTTGCGGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTg                           <  1:1183813/148‑1 (MQ=255)
                                                                                                                        cAAGAAGGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCAtt                     >  1:532513/1‑149 (MQ=255)
                                                                                                                          agaagGAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTcc                   >  2:1065725/1‑149 (MQ=255)
                                                                                                                              ggAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGc               >  1:994302/1‑149 (MQ=255)
                                                                                                                              ggAATCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCACGGGAATATTGACGGGCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCGATTCCCAGc               >  1:674982/1‑149 (MQ=255)
                                                                                                                                  tCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCtt           <  1:1214934/149‑1 (MQ=255)
                                                                                                                                  tCGACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGAAATATTGACGGTCGGCAAGCAACTTTTGCAGGCCGTTAATTTGCTCCAGGCGCAGCACTTCGTCGCTGTCCATTCCAAGCTCtt           <  2:15099/149‑1 (MQ=255)
                                                                                                                                    gACGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTg         <  1:891234/149‑1 (MQ=255)
                                                                                                                                      cGTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGcc       <  2:1100162/149‑1 (MQ=255)
                                                                                                                                       gTTGCTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTATCCCTTCCCAGCTCTTTGCCa      >  2:974996/1‑149 (MQ=255)
                                                                                                                                           cTGGTAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAAttt  >  1:740645/1‑149 (MQ=255)
                                                                                                                                           cTGGTAGCAGCTGAACGCCAGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACTCCTCTTGCAGGCCGTTAATTTGCTCCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAAttt  <  1:806741/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TACCCTGGATGATGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTT  >  CP009273/630031‑630318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑