Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,142,095 G→A 14.9% R314H (CGC→CAC)  yegD → Hsp70 chaperone family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,142,0950GA14.9% 60.7 / 2.0 27R314H (CGC→CAC) yegDHsp70 chaperone family protein
Reads supporting (aligned to +/- strand):  ref base G (16/7);  new base A (2/2);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GTATGGGTGGCGAAACCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAG  >  CP009273/2141960‑2142239
                                                                                                                                       |                                                                                                                                                
ggaTGGGGGGCGACACCGAAAAAGGCATCGCCCGGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCcg                                                                                                                                         <  1:241924/143‑1 (MQ=255)
gTATGGGTGGCGAAACCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCcg                                                                                                                                         <  2:6224/145‑1 (MQ=255)
    gggTGGCGAAACCGAAAAAGGCATAGACCGGCCGATCCTACAGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTTCCAACGGTCGTCTGCTTAACGATCTGGTAGGGGATTccctagaa                                                                                                                                     >  1:409037/1‑140 (MQ=255)
           gAAACCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGaaa                                                                                                                              >  2:229759/1‑145 (MQ=255)
             aaCCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGCGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACACGATGCCCGCGAACCGGaaaaa                                                                                                                            >  2:15679/1‑145 (MQ=255)
             aaCCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACACGATGCCCGCGAACCGGaaaaa                                                                                                                            >  2:15651/1‑145 (MQ=255)
              aCCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAg                                                                                                                           <  2:59958/145‑1 (MQ=255)
                  tgaaaGGCACCGGCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACTTGCGCAGGGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCAAACCGTAAAAAGGGGc                                                                                                                       <  1:261107/143‑1 (MQ=255)
                           tCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACa                                                                                                              >  2:409968/1‑145 (MQ=255)
                                      aTCCTGCCGTGTTGGGATGCGGTTGCCATCAACGCTGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGGCGTCTGCTTATCGATCTGGTACGCGATGCCTGCGAACCGGAAAATGTGGCCCTGTTACAGAAAGTCTGGc                                                                                                   <  1:176712/145‑1 (MQ=255)
                                            ccGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCAGGGGAACTGGGAAATGTGGCGCAGTTACAGAAAGTCTGGCGTCAGc                                                                                             >  2:116788/1‑145 (MQ=255)
                                                   ggAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTGCAGTAGGGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTAAAGCAAGTCTGGCGTCAGCGTTTAAg                                                                                      >  1:279211/1‑145 (MQ=255)
                                                   ggAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGATACAGAAAGTCTGGCGTCAGCGTTTAAg                                                                                      >  2:344975/1‑145 (MQ=255)
                                                   ggAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGGAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGCACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAg                                                                                      <  2:418040/145‑1 (MQ=255)
                                                                cATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCACCGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGGGGCCCTGTTACAAAAAGTCTGGCGTCCGCGTTTAAGCGATCGccctgcg                                                                         >  1:55750/1‑140 (MQ=255)
                                                                cATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGATTAAAGATATGGCCCGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCCGGTg                                                                         >  1:108838/1‑145 (MQ=255)
                                                                    aaCGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTa                                                                     >  1:208627/1‑145 (MQ=255)
                                                                          gTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACACGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCag                                                               <  1:15679/145‑1 (MQ=255)
                                                                          gTACCGGCGCGGAGTGGTTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACACGATGCCCGCGAACCGGAAAAAGTGGCCCTGGTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCag                                                               <  1:15651/145‑1 (MQ=255)
                                                                               tGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAagag                                                          >  2:50816/1‑145 (MQ=255)
                                                                               tGCGCAGAGGGATTTCTACAGTAGTGCCAACGCTCGTCTGCTTGACGATCTGGTACGCGATGCACGCGAACCGGAAAAGGTGGCCCTCTTACAGAAAGTCTGGCGGCAGCGTGTAAGCTAGCGCCTGGACGGGAGCGCcgaagcg                                                          >  1:394220/1‑143 (MQ=255)
                                                                                 cgcAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTg                                                        >  1:202718/1‑145 (MQ=255)
                                                                                          aTTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAACGCAGCAGAGTGCAAAATTGc                                               >  1:350066/1‑145 (MQ=255)
                                                                                                          cAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCg                               >  2:128008/1‑145 (MQ=255)
                                                                                                                      gCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGcc                   >  1:258160/1‑145 (MQ=255)
                                                                                                                              aTCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGcc           <  2:138805/145‑1 (MQ=255)
                                                                                                                               tCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCg          >  2:418679/1‑145 (MQ=255)
                                                                                                                                       gcgATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCCCTGCCGTTTATCAg  >  2:407460/1‑145 (MQ=255)
                                                                                                                                       |                                                                                                                                                
GTATGGGTGGCGAAACCGAAAAAGGCATCGCCCTGCCGATCCTGCCGTGGTGGAATGCGGTTGCCATCAACGACGTACCTGCGCAGAGTGATTTCTACAGTAGTGCCAACGGTCGTCTGCTTAACGATCTGGTACGCGATGCCCGCGAACCGGAAAAAGTGGCCCTGTTACAGAAAGTCTGGCGTCAGCGTTTAAGCTATCGCCTGGTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAG  >  CP009273/2141960‑2142239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑