| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,371,135 | G→T | 25.0% | intergenic (‑356/+39) | sspA ← / ← rpsI | stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,371,135 | 0 | G | T | 25.0% | 35.3 / 6.2 | 24 | intergenic (‑356/+39) | sspA/rpsI | stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9 |
| Reads supporting (aligned to +/- strand): ref base G (11/7); new base T (2/4); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACG > CP009273/3370997‑3371272 | ttAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTATTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑tttttt > 2:302983/1‑145 (MQ=255)gtAGTCTGTAATAGTTGCCGTCCTCCCTTATTGGCTACGGTTCTTTTGTTTCTCTTCTCTGTCAGGCTGCGCCAAATAGCAACCTTTCTGTTTTTACTCTTATTCATATTTTTGCTATAAAAAAACCCGCCGAAGCGGG‑tttttt < 1:58563/144‑1 (MQ=255) tCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAACAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGaa > 2:204088/1‑145 (MQ=255) tGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGaaaa < 2:390789/145‑1 (MQ=255) cTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTGTTACGCTGATTCAGGTGTTAGCTATAAAAAAACCCGCCGAAGCGGT‑TTTTTTCGAAAATTGTTTTCTGcc > 1:345388/1‑145 (MQ=255) gtgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTACTGCCGGAGCAGAAGCCAATTAACg < 2:253608/145‑1 (MQ=255) ggggttttggttttACCTATCTGTCAGGTAGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCACATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACg < 1:71142/143‑1 (MQ=255) tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGCGGCATAAGCCAAGTACCGt > 2:152980/1‑145 (MQ=255) gccAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGT‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga < 2:265636/143‑1 (MQ=255) gTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGc > 2:318793/1‑145 (MQ=255) gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCAGCGGAGGGGGG‑TTTTTTCGAAAGTTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGGGCttt > 1:234169/1‑145 (MQ=255) gcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGGGCTTTACg > 2:244656/1‑145 (MQ=255) aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCCGAAGCCAAATAACGCTTGGCGAACTACGgaagacgacatacgatacgcc > 2:240401/1‑133 (MQ=255) aaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa > 1:361040/1‑145 (MQ=255) aaGCAACTTTCCAGTTTGTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAc < 1:163718/145‑1 (MQ=255) gCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCCGACCg > 2:298543/1‑145 (MQ=255) tttCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGGGGACGACGACGTGCTTTACGCAGACCGACctt > 1:43776/1‑145 (MQ=255) tCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGT‑TCTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt < 1:304457/145‑1 (MQ=255) aGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGT‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGAAGAACTGCGGACGACGACGTGCTTTACGCAGACAGACTGTCTTAc > 2:257123/1‑145 (MQ=255) gTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACg < 1:130931/145‑1 (MQ=255) tacgctagtccaaTTTTAGGAATAAAAAAACCCGGCGAAGCGGTTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa < 2:263274/133‑1 (MQ=255) ataAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAAc > 1:362262/1‑145 (MQ=255) aaaaaaaCCCGCCGAAGCGGT‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGa < 1:305366/145‑1 (MQ=255) gAAGCGGG‑GTTTTGCGAAAATGGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACg < 2:83969/145‑1 (MQ=255) | TTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGG‑TTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACG > CP009273/3370997‑3371272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |