| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,419,720 | T→C | 45.1% | D183G (GAT→GGT) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,419,720 | 0 | T | C | 45.1% | 5.3 / 46.6 | 48 | D183G (GAT→GGT) | hisJ | histidine/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (11/13); new base C (13/8); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTA > CP009273/2419579‑2419861 | tCGCGCAGTTCGTTTTCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCt < 2:209296/145‑1 (MQ=255) cgcgCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGACTTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCACTGGCAGCGACAACATCCTg > 2:328142/1‑145 (MQ=255) gTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcgc > 1:333265/1‑145 (MQ=255) gTTCGTTATCTTCTTTACGCAGGCCCATGCCGGGCCCTACGCCAAACAGTAAATCAACTTTAACAGACGGGCCACCGAGCTGGTACTCATCACCGACAGGTTGTTTCAGGACACCTTCGCTGCCAGCTACCTCACCCTCGAAggc > 1:333402/1‑145 (MQ=255) ttCGTTATCTTCTTTACGCAGGCTCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcgcg < 2:295550/145‑1 (MQ=255) cGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGc < 1:232185/145‑1 (MQ=255) ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTGATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCGGCGCCCTCACCCTGGAACGCGGCATCaaac > 1:339473/1‑143 (MQ=255) tcttTACGCAAGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCCTCTTTAACAGGCGGGCCACCGAATTTGTAATCTTTCCCGACAGGTTGGTTGAGGAACCCTTCGCTGGCGGCGACCTCACCATGGAAGGCGGCATCacaac > 1:60878/1‑141 (MQ=255) ccttGCGGGTCCTTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtcag < 2:159413/142‑1 (MQ=255) ccTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCCGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa < 2:299324/145‑1 (MQ=255) ccTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACAGACAGGTTGTTTGAGAAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCCATACGTCCGGCAGTCAGGGCAGCATaaa > 1:159760/1‑145 (MQ=255) cTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCAGCAATACGTCGGGCAGTAAGGTCAGAATaaag > 1:243198/1‑144 (MQ=255) atACGCCAAACAGTTTTTCCTCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAACGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAt < 1:271159/144‑1 (MQ=255) cGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgtt < 1:66753/145‑1 (MQ=255) cGCCAAACAGTTTATCACCTTTAACAGACGGGCCACCTAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgtt < 1:330316/145‑1 (MQ=255) cAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCCGCGACCTCAGCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTc < 1:69837/145‑1 (MQ=255) aaCAGTTTTTCATTTTTAATAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTcat > 1:380994/1‑143 (MQ=255) tttttCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGcccc < 2:155672/145‑1 (MQ=255) ttttCATTTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACTTTCGCTTGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCATGTTAGAATAAATGTTGTCCTGCCCCt < 2:309265/145‑1 (MQ=255) ttttCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCCCCCTGGACCGCGGCCTCACTTCCTCCGGCAGTCCGGTCAGATTAAATTTGGTGCTGTCCCt < 1:144242/145‑1 (MQ=255) tCTTTAACAGACGCGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCGGAATAAATGTTGTCCTGCCCCTGATACg > 2:289035/1‑145 (MQ=255) tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATCACTGTTGTCCTGCCCCTGATACgag > 2:200226/1‑145 (MQ=255) gACGGGCCACCGAATTTGTAATTTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCCGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAttt < 2:73990/145‑1 (MQ=255) aCGGGCCACCGAATTTGTAATCTTTACCGACAGGGTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTc < 2:371692/145‑1 (MQ=255) gggCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCaa < 2:251436/145‑1 (MQ=255) ccGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCtt > 2:244393/1‑145 (MQ=255) cgacattttAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATCCGAGACGATTTCAATGCCttt > 1:157889/9‑145 (MQ=255) aaTTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTg < 2:199094/145‑1 (MQ=255) tGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGc < 2:106542/145‑1 (MQ=255) gTAATCTTTACCGACGGGTTGTTTGAGGAAACCTTCGCGGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGGCAGGTGAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGcc < 1:210365/145‑1 (MQ=255) tAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTCGTGccc > 2:157432/1‑145 (MQ=255) aCCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCa < 1:302974/145‑1 (MQ=255) cGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCAtt > 2:31786/1‑145 (MQ=255) cGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCAtt > 1:17053/1‑145 (MQ=255) atggTTATTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTAcc < 2:181494/143‑1 (MQ=255) gttTGAGGAAGCCTTGTCTGGGAGCTTCCTCAGCCTGGAATGCGGCATCAATACGTCCGGTAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGt < 2:8335/145‑1 (MQ=255) gttTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGt > 2:261872/1‑145 (MQ=255) ttGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGACAGAAGAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGgct > 1:115718/1‑145 (MQ=255) gtGAGCAAACCCTCGCTGGCAGCGACCTCAGCCTGGAACGCGGCATCCATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGCTTTCAATACCTTTTGGTGCCCACTGTTCATTACGGAACGtac > 1:82756/2‑143 (MQ=255) ggAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTg > 2:403035/1‑145 (MQ=255) aCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggtg < 2:400243/145‑1 (MQ=255) ttCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCACAATGTTCATTACCGAACGTCTCCTGGgtggtg > 2:287380/1‑145 (MQ=255) cTGGCAGCGACCTCATGCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCt > 2:266422/1‑145 (MQ=255) cTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCt > 1:369428/1‑145 (MQ=255) gCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGTGGCCCTGCAATa > 2:23554/1‑145 (MQ=255) aCCTCCCCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATACATTTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGc < 1:363326/145‑1 (MQ=255) ccTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCAAATACGAGACGATTTCAATGCCTTTTGGTGCCCCATGTTCATTACCGAAGCTCTCCTGGGGGGCGCCCAGGAATaggac > 1:341021/1‑141 (MQ=255) tCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCAGGGTGGTGCCCTGCAATACGCCTa > 2:130517/1‑145 (MQ=255) | TCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTA > CP009273/2419579‑2419861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |