Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP009273 3,528,829:1 (GTTTTCAGCCAG)1→2 88.6% coding (399/1353 nt) envZ ← sensory histidine kinase in two‑component regulatory system with OmpR

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 35288103 (0.150)23 (1.090)
+CAGCCAGGTTTT
18/206 NT 88.6% coding (418/1353 nt) envZ sensory histidine kinase in two‑component regulatory system with OmpR
?CP009273 3528811 = 3 (0.130)coding (417/1353 nt) envZ sensory histidine kinase in two‑component regulatory system with OmpR

CGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC  >  CP009273/3528673‑3528951
                                                                                                                                          |                                                                                                                                            
gatacgaataaacagccacgccccgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgactttcgggaagcggcacgcgaacccaaatattaccccacagccaggttttcagccag                                                                                                                                        >  1:173473‑M2/139‑145 (MQ=255)
     gaataaacagccacgccccgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcccgcgtacccagaaattgggcgacagccaggttttcagccaggattt                                                                                                                                   >  2:70358‑M2/134‑145 (MQ=255)
           acagccacgccacgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttcagccaggttttcagcca                                                                                                                             <  1:253289‑M2/18‑1 (MQ=255)
             agccacgccccgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAga                                                                                                                           >  2:146507‑M2/126‑145 (MQ=255)
             agccacgccccgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAga                                                                                                                           >  2:146497‑M2/126‑145 (MQ=255)
             agccacgccccgcctagcgccattagcataatcgccagcgtatagcggaactgcggagagaaatcgccctggtgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAga                                                                                                                           <  2:344713‑M2/20‑1 (MQ=255)
              gcaacgccccgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAgac                                                                                                                          <  1:224877‑M2/21‑1 (MQ=255)
                 gAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGCTTTCAGCCAgaatat                                                                                                                       >  2:70441/1‑141 (MQ=255)
                    ccccgcctatcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAgg                                                                                                                    <  1:379621‑M2/27‑1 (MQ=255)
                             tcgccaatagcataatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatatcggtcagcggaacgcgtacccagctattgggcgacagccaggggttCAGCCAGGGCTTCAGCCAGACGACAGGCGAACtttt                                                                                                           >  1:63575‑M2/110‑145 (MQ=255)
                                     ccgaTCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCATCCAGACGACAGGCGAACTTTTGTTGACCt                                                                                                   <  2:73011/142‑1 (MQ=255)
                                         taatcgccagcgtatagcggaacagcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgccagccagggtttCAGCCAGGTGTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAAc                                                                                               >  2:226911‑M2/98‑145 (MQ=255)
                                             cgccagcgtatagcggaacatcggagagaaatcgtcctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACgcgc                                                                                           <  2:393606‑M2/52‑1 (MQ=255)
                                                             accagcggagcgaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgaacgccaggttttCTGCCAGGTTTTCAGCCAGACGACAGGCGCACTTTTGTTGACCTCAAGCCGCACTTCCGTCGGgccgc                                                                           >  1:203237‑M2/78‑145 (MQ=255)
                                                              acagcggagagaaatcgcccggatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGgccgcc                                                                          <  1:57913‑M2/69‑1 (MQ=255)
                                                                agcggagagaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCa                                                                        >  1:226295‑M2/75‑145 (MQ=255)
                                                                       agaaatcgccctgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccagtttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTtgctg                                                                 <  1:389630‑M2/78‑1 (MQ=255)
                                                                                  tgatgaatttcggtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGAt                                                      >  2:47556‑M2/57‑145 (MQ=255)
                                                                                              gtcagcgtcacgcggtcccagaaattgggcgacagccaggttttCAGCCTGTTTTTCAGCCAGACGACAGGCGAACTGTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt                                          <  1:96935‑M2/101‑1 (MQ=255)
                                                                                              gtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt                                          <  2:371078‑M2/101‑1 (MQ=255)
                                                                                              gtcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt                                          <  2:354480‑M2/101‑1 (MQ=255)
                                                                                              gtaagcggcacgcgtacccagatattgggcgacagccaggttgtCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCCCAACGCGCACTCCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAAtt                                          <  2:177030‑M2/101‑1 (MQ=255)
                                                                                               tcagcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTc                                         >  2:224261‑M2/44‑145 (MQ=255)
                                                                                                  gcggcacgcgtacccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCatg                                      >  1:240717‑M2/41‑144 (MQ=255)
                                                                                                       ccgggtacccatatcttgggccacagccaggttttCAGCCAGGTTTTCCGCCAGACGACAGGCCAACTTTTGTTGGCCTCGACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGtt                                 <  1:68605‑M2/110‑1 (MQ=255)
                                                                                                               ccagatattgggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTATTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCaa                         >  2:282485‑M2/28‑145 (MQ=255)
                                                                                                               ccagatattgggcgacagccaggttctCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCaa                         >  2:374081‑M2/28‑145 (MQ=255)
                                                                                                               cGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTGCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCaa                         <  2:173473/145‑1 (MQ=255)
                                                                                                                         ggcgacagccaggttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACATCCTTCGGGCCGCCCAGTTGCTGCGCCACCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTg               <  2:63575‑M2/128‑1 (MQ=255)
                                                                                                                                gcctggttttCAGCCATGTTTTCAGCCATACTATAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCACCTGATGGCTTAAGAACTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTc        <  2:380150‑M2/135‑1 (MQ=255)
                                                                                                                                     gttctCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAg   <  1:374081‑M2/140‑1 (MQ=255)
                                                                                                                                      ttttCAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGc  <  1:47556‑M2/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CGGTCGGTTCTGGATACGAATAAACAGCCACGCCCCGCCTATCGCCAATAGCATAATCGCCAGCGTATAGCGGAACAGCGGAGAGAAATCGCCCTGATGAATTTCGGTCAGCGGCACGCGTACCCAGATATTGGGCGACAGCCAGGTTTTCAGCCAGACGACAGGCGAACTTTTGTTGACCTCAACGCGCACTTCCGTCGGGCCGCCCAGTTGCTGCGCCATCTGATGGCTTAAGAATTCATAGTGTTGCGCCCAACGCAGACCTGCCTCTTCGGCAGC  >  CP009273/3528673‑3528951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.