Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,215,619 IS2 (+) +5 bp 33.3% intergenic (‑151/‑41) bglX ← / → dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2062422 =NA (NA)6 (0.260) 6/268 NT 39.1% noncoding (1331/1331 nt) IS2 repeat region
?CP009273 2215619 = 9 (0.400)intergenic (‑151/‑45) bglX/dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent
* ? CP009273 = 2063752NA (NA)3 (0.130) 3/268 NT 24.3% noncoding (1/1331 nt) IS2 repeat region
?CP009273 = 2215623 9 (0.400)intergenic (‑155/‑41) bglX/dld beta‑D‑glucoside glucohydrolase, periplasmic/D‑lactate dehydrogenase, FAD‑binding, NADH independent

TTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2063609‑2063752
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AATAGCGCCTTGTATAACAATTCAGCGAGTGAATATTTTGAAAATTCAGACAATAAACTGAGCATTCTTTTTCTCTCTCCATCATGCTTATGGCAAA  <  CP009273/2215623‑2215527
                                                                                                                                                                                                                                                 
TTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAA                                                                                                  >  1:363159/1‑145
TTTCCCTGTGAGGTACGCAGTCTGCGTTTCTCCGGCCCTAAGACACCAATCATCTGTTCTCCAATGACTAGGCGAAAAACTAGTAGGAAGACGATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAA                                                                                                  <  2:194499/145‑1
                                              CAATCATCCGTGCTCCACTGTCTAGTCTAAAGACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAATAGCGCCTTGTATAACAATTCAGCGAGTGAATATTTTGAAAATTC                                                    <  1:120558/145‑1
                                                       GTTCTCCAATGACTAGTCTAAAAACTAGTCTTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGAGCCAGTCTAAATAGCGCCTTGTATAACAATTCAGCGAGTGAATATTTTGAAAATTCAGACAATAA                                           >  2:335892/1‑145
                                                                                                ACTTATTTTAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAATAGCGCCTTGTATAACAATTCAGCGAGTGAATATTTTGAAAATTCAGACAATAAACTGAGCATTCTTTTTCTCTCTCCATCATGCTTATGGCAAA  <  1:122688/145‑1
                                                                                                                                                                                                                                                 
TTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2063609‑2063752
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AATAGCGCCTTGTATAACAATTCAGCGAGTGAATATTTTGAAAATTCAGACAATAAACTGAGCATTCTTTTTCTCTCTCCATCATGCTTATGGCAAA  <  CP009273/2215623‑2215527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.