Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,705,839 G→A 6.0% G152G (GGC→GGT)  yhjY ← autotransporter beta‑domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,705,8390GA 6.0% 201.9 / 2.4 67G152G (GGC→GGT) yhjYautotransporter beta‑domain protein
Reads supporting (aligned to +/- strand):  ref base G (27/36);  new base A (2/2);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCG  >  CP009273/3705694‑3705980
                                                                                                                                                 |                                                                                                                                             
tgaGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCa                                                                                                                                            <  1:99693/149‑1 (MQ=255)
  aGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAg                                                                                                                                          <  1:213483/149‑1 (MQ=255)
   gCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCGAAGc                                                                                                                                         >  1:77750/1‑149 (MQ=255)
   gCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATCCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGc                                                                                                                                         <  1:208347/149‑1 (MQ=255)
    cAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGATGGTCGTCGGGGTTTTGGGCCCAGGGTCGGGGATCGCCACGcc                                                                                                                                        >  1:68589/1‑149 (MQ=255)
        atatCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTCGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGa                                                                                                                                    <  1:256423/149‑1 (MQ=255)
          atCAGCGCCTACGGTGACACCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGt                                                                                                                                  <  1:291803/149‑1 (MQ=255)
           tccgcgcCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCg                                                                                                                                       <  2:33531/140‑1 (MQ=255)
             aGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCaa                                                                                                                               <  1:262345/149‑1 (MQ=255)
                gcCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAacac                                                                                                                            <  1:302203/149‑1 (MQ=255)
                 ccTGCGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGCCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGGTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTc                                                                                                                                 <  2:5977/143‑1 (MQ=255)
                   tcgggTGCCTTCCAGCCGGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGGTATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGGGCGCCAAACCGGGGGTCaa                                                                                                                               <  2:120531/140‑1 (MQ=255)
                   tACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCaa                                                                                                                               <  2:239590/143‑1 (MQ=255)
                   tACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGcc                                                                                                                         <  1:100930/149‑1 (MQ=255)
                   tACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCCTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCaa                                                                                                                               <  2:125938/143‑1 (MQ=255)
                    aCGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCa                                                                                                                        <  1:181169/149‑1 (MQ=255)
                     cGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAaca                                                                                                                             <  2:322975/143‑1 (MQ=255)
                      ggTGACATCCAGCCAGTTGCCGTTCTGGTTTGTTGCCTTCATCCGGCTCAGGCCTGATTGCGCCTACCAGATATTCTCGCCAAATTGCTGGTTATAGCAGATTTGCGCCCAGGGTCGTGGATCGCCAAGCAGGGAGTCGACACGCCAGc                                                                                                                      >  1:236086/1‑149 (MQ=255)
                        tGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCt                                                                                                                    >  1:309979/1‑149 (MQ=255)
                            aTCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCGTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAgg                                                                                                                >  1:207291/1‑149 (MQ=255)
                            aTCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAaccccccggc                                                                                                                      >  2:8599/1‑135 (MQ=255)
                            aTCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAgg                                                                                                                >  1:50272/1‑149 (MQ=255)
                            aTCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATATGCGCCCCGGGTCGGAGATCGCCAAGCCGGGGGTCGACACGGCAGc                                                                                                                      >  2:180234/1‑143 (MQ=255)
                                 gccagTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTc                                                                                                           <  1:297341/149‑1 (MQ=255)
                                    agTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACg                                                                                                        >  1:118002/1‑149 (MQ=255)
                                       tGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTTGCCAAATTGCTGGTTATAGCTGATATGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGGCAACACGCCAGCCTAAGGTACTCACGCTg                                                                                                     <  1:239429/149‑1 (MQ=255)
                                         ccGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGACAAGCCGGGAGTCAACACGCCAGCCTAAGGTACtgcc                                                                                                         >  2:168239/1‑140 (MQ=255)
                                         ccGTTCTGGTTTGTCGCCGTCAGCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCAc                                                                                                         <  2:241076/143‑1 (MQ=255)
                                            ttCTGGTTTGTCGCCGTCGTCCGGCTCAGGCCTGATTGCGCCTTCCAGGTATTCTCGCCAAATTGGTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCt                                                                                                      <  2:202396/143‑1 (MQ=255)
                                            ttCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCt                                                                                                      >  2:180157/1‑143 (MQ=255)
                                            ttCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAAAACGCAGGCCGACGGTACTCACGCTGGCCTg                                                                                                >  1:277645/1‑149 (MQ=255)
                                                  tttGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCACCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATaa                                                                                          >  1:286197/1‑149 (MQ=255)
                                                    tgtgGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAACTGCTGGTTATAGCTGATTTGCTTCCAGGGTCGGAGATCACCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACg                                                                                        <  1:42285/149‑1 (MQ=255)
                                                    tGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACg                                                                                        >  1:158891/1‑149 (MQ=255)
                                                    tGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCGGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCACGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCAAGcc                                                                                              >  2:248825/1‑143 (MQ=255)
                                                         ccGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAAc                                                                                         >  2:222573/1‑143 (MQ=255)
                                                            tCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTc                                                                                >  1:62066/1‑149 (MQ=255)
                                                            tCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTc                                                                                >  1:243911/1‑149 (MQ=255)
                                                            tCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGAGCGGTc                                                                                >  1:330208/1‑149 (MQ=255)
                                                               tCCCGCCCAGGCCTGGTTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCg                                                                                   <  2:323801/143‑1 (MQ=255)
                                                                  ggCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTc                                                                                >  2:242814/1‑143 (MQ=255)
                                                                    cTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATCCCATAACGGAGCGGCCAGCGGGgt                                                                        >  1:319913/1‑149 (MQ=255)
                                                                     tccggCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGgta                                                                       <  1:169464/146‑1 (MQ=255)
                                                                     tCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGgta                                                                       <  1:27730/149‑1 (MQ=255)
                                                                          ccTGATTGCGCCTTCCAGATATTCTCGCCACATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACTCGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACt                                                                  >  1:192216/1‑149 (MQ=255)
                                                                             gATTGCGCCTTCCAGATATTCTCGCCAACTGGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGTTACTCACGCTCGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTc                                                               <  1:205803/149‑1 (MQ=255)
                                                                              atcgcgcCTGCCAGATTTGCTCGCCAAATTGCTGGTTATAGCCGATTTTCGCCCAGGGTCGGCGATCGCCAAGCCGGGAGTCAACCCGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCg                                                              <  1:139867/146‑1 (MQ=255)
                                                                                    ccTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAAc                                                        >  1:199778/1‑149 (MQ=255)
                                                                                      ttCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGCGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGcc                                                            >  2:35481/1‑143 (MQ=255)
                                                                                        ccAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGt                                                    <  1:242084/149‑1 (MQ=255)
                                                                                            atatTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTc                                                      <  2:219090/143‑1 (MQ=255)
                                                                                             tatTCTCGCCAAATTGCTGGTTACAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCg                                                     <  2:208342/143‑1 (MQ=255)
                                                                                                  tcGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTtata                                                <  2:178695/143‑1 (MQ=255)
                                                                                                  tcGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCATCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGa                                          >  1:338288/1‑149 (MQ=255)
                                                                                                        aaTTGCTGGTTATAGCTGACTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGa                                          <  2:304891/143‑1 (MQ=255)
                                                                                                         aTTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAg                                         <  2:260214/143‑1 (MQ=255)
                                                                                                           tGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCATACTCGTTATACATCGAGGTGGTtgt                                 <  1:190842/149‑1 (MQ=255)
                                                                                                             cTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAggtgg                                     >  2:170331/1‑143 (MQ=255)
                                                                                                                gTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGATGTGCCac                            >  1:167204/1‑148 (MQ=255)
                                                                                                                     tgCTGATTTGCGCCCAGGGTCGGAGATCACCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCa                             <  2:256672/142‑1 (MQ=255)
                                                                                                                          aTTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCgtaatgtaa                  <  1:210497/149‑1 (MQ=255)
                                                                                                                          aTTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCgtaatgtaa                  >  1:217204/1‑149 (MQ=255)
                                                                                                                                gcCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACACCGAGGTGGTTGTGCCATCGTAATGTAATGCcgc            >  1:119063/1‑149 (MQ=255)
                                                                                                                                      ggTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCgg      <  1:81444/149‑1 (MQ=255)
                                                                                                                                              aTCGCTAATCCGGTAGTCAACACGCCAGCCTAAGGCACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACCCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGc    <  2:299321/143‑1 (MQ=255)
                                                                                                                                               tCGCCAAGCCGGGAGTCAACACGCCATCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTTGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGcc   <  2:183448/143‑1 (MQ=255)
                                                                                                                                                cGCCAAGCCGGGAGTCAACACGCCGGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCg  <  2:176430/143‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCG  >  CP009273/3705694‑3705980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑