| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 444,779 | C→T | 100% | W440* (TGG→TAG) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 444,779 | 0 | C | T | 91.7% | 46.3 / ‑3.7 | 18 | W440* (TGG→TAG) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (7/9); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
GCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCA > CP009273/444646‑444899 | gcgcAAACGTCGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATcc < 1:7002/146‑1 (MQ=255)gCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATcc > 1:349704/1‑149 (MQ=255) ggTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGCAGAAGCCGATGATCCAGCACCAGAACGCGCGTTTACCCCCGGTTTCGTTCAGTTTGAAACCGAACGCGTTAGGCCACCAGTAGGGCATCCCTGCgg > 2:176195/1‑142 (MQ=255) tGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGGCCAcg > 1:243958/1‑149 (MQ=255) cccAGCGCATACCGTGGCATAAAGCCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGGTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACgccgcc < 1:134054/149‑1 (MQ=255) gcATCCAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatgat < 1:190520/149‑1 (MQ=255) gcATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatgat < 1:229168/149‑1 (MQ=255) gcATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatgat < 1:355784/149‑1 (MQ=255) cAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCgatga > 2:64721/1‑143 (MQ=255) tAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTa < 2:162687/143‑1 (MQ=255) aaCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGt < 2:305340/143‑1 (MQ=255) gCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCAGATGATCACGTTATGGAAGTGCGCAATCAGGAACa > 1:205734/1‑149 (MQ=255) ccGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCCCGTGATGGAAGCGCGCaagccg > 2:269778/1‑139 (MQ=255) cagaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCt < 2:134125/143‑1 (MQ=255) ccagaacGCGCGTCTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGaa < 1:71985/149‑1 (MQ=255) tCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcag > 1:260542/1‑149 (MQ=255) tttGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTATTATGCAGAACGAAGTCCGCGCCCGGCACGGCcagcc > 2:33361/1‑142 (MQ=255) cttGAACCCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagca < 2:315707/142‑1 (MQ=255) | GCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCA > CP009273/444646‑444899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |