Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 59.1% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)17 (0.860) 16/270 NT 64.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 9 (0.470)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)9 (0.450) 8/270 NT 49.2% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 9 (0.470)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

CTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACT  >  CP009273/2400113‑2400408
                                                                                                                                                    |                                                                                                                                                    
aactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                                      <  1:119986‑M2/4‑1 (MQ=255)
aactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                                      >  1:79782‑M2/146‑149 (MQ=255)
aactggggagaacgccttaaatggcgaacaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                                      <  1:273239‑M2/4‑1 (MQ=255)
     cATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaagg                                                                                                                                                >  1:340179‑M2/1‑143 (MQ=255)
      ggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGc                                                                                                                                                <  1:96316‑M2/10‑1 (MQ=255)
           cgatcaTTTACTGGCGGCTCATACTGGGCGGGTTAATGCTGGGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAGCCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaa                                                                                                                                          >  1:95073‑M2/3‑137 (MQ=255)
               cttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACAt                                                                                                                                       >  1:202375‑M2/131‑149 (MQ=255)
                     tggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACAAAGCGACTCACAt                                                                                                                                       >  2:223142‑M2/125‑143 (MQ=255)
                        gcggcTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTAGCCGTTTCATCCGGAACCCGGCCGAGAGTAAACATGCATCTGCGTTGCACTCAggaagtagcgcgcaagtccctgcca                                                                                                                             <  1:77129‑M2/149‑26 (MQ=255)
                                 tattgtgtGGTTAAATGCTTTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGGtata                                                                                                                           >  2:250362/8‑143 (MQ=255)
                                  gtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagcagagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATaa                                                                                                                          >  2:36126‑M2/112‑143 (MQ=255)
                                              tgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATcaca                                                                                                        >  1:351062‑M2/100‑149 (MQ=255)
                                                attcaaggcatgtacgggagaaaaaagcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGa                                                                                                      <  1:2192‑M2/52‑1 (MQ=255)
                                                  tcaaggcatttatgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATca                                                                                                          <  2:293761‑M2/48‑1 (MQ=255)
                                                       gcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATc                                                                                               <  1:223099‑M2/59‑1 (MQ=255)
                                                       cacacacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGaa                                                                                                     <  2:124560/143‑1 (MQ=255)
                                                            acaaacaaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAg                                                                                          >  1:242720/1‑149 (MQ=255)
                                                                 caaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATc                                                                                     <  1:76061/149‑1 (MQ=255)
                                                                  agaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGACGTACCTCACAAAATATATCTTCAgggaact                                                                                    >  1:174856‑M2/80‑146 (MQ=255)
                                                                  agaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCg                                                                                    >  1:358416‑M2/80‑149 (MQ=255)
                                                                   gaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATACCTTCAgg                                                                                         >  2:315033‑M2/79‑143 (MQ=255)
                                                                        ttCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgagaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatga                                                                              >  1:246210‑M2/1‑77 (MQ=255)
                                                                          ccATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatc                                                                                  >  2:305955‑M2/1‑75 (MQ=255)
                                                                              ttGTAACGTGCCTCCCGGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatga                                                                              >  2:283866‑M2/1‑71 (MQ=255)
                                                                                 aaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGtt                                                                     >  1:98876‑M2/65‑149 (MQ=255)
                                                                                    tgtgCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCAAATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTact                                                                  >  1:253442/1‑149 (MQ=255)
                                                                                         cTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaactt                                                                   >  2:228474‑M2/1‑60 (MQ=255)
                                                                                                       cATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGa                                               <  1:79974/149‑1 (MQ=255)
                                                                                                        aTCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACCAACATTGAGCCGGTATAATGCCTGCAGTACATAGATCTCAACAACTTCATATAGCGCAGATCTATTAAGTTACTCACTCTTTTCTAtt                                                    >  2:232585/1‑143 (MQ=255)
                                                                                                          gtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTa                                                  <  2:316977‑M2/104‑1 (MQ=255)
                                                                                                              gccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGAc                                              >  2:21424‑M2/36‑143 (MQ=255)
                                                                                                                     aCGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGCGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGcgcg                                       <  2:79630/143‑1 (MQ=255)
                                                                                                                       gcaagtAAAAATGAATATGAGTTGCACTACggaaggtgcgaaccagtccctgatatgagctcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgt                               >  1:351497‑M2/3‑30 (MQ=255)
                                                                                                                            ccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGt                                >  2:307289‑M2/22‑143 (MQ=255)
                                                                                                                             aaaaaTGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGta                               <  2:20176/143‑1 (MQ=255)
                                                                                                                                 cttattcgcaccttccCTAAAAAAGCGGCTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaa                           >  2:277459‑M2/17‑143 (MQ=255)
                                                                                                                                       cgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg               >  1:281688‑M2/11‑149 (MQ=255)
                                                                                                                                            ctccactAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttc          >  1:126281‑M2/4‑9 (MQ=255)
                                                                                                                                             ttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg               >  2:182285‑M2/5‑143 (MQ=255)
                                                                                                                                                 tttaggaatgtgcgaacaagtcctggatatgagatcatgtttgtcatctggagccatcgaacagggttcatcctgagtcatcaaattaccttcgccgacagtgaattcagaagtaagcgccgtcagaccagaaaagagattttcttgtc     >  1:36163‑M2/4‑4 (MQ=255)
                                                                                                                                                 cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggggccagagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtc     <  1:346766‑M2/149‑146 (MQ=255)
                                                                                                                                                 cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagatttt           >  2:274302‑M2/1‑4 (MQ=255)
                                                                                                                                                 cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagatttt           >  2:320690‑M2/1‑4 (MQ=255)
                                                                                                                                                  tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttc          >  2:301102‑M2/1‑3 (MQ=255)
                                                                                                                                                  tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttc          >  2:106948‑M2/1‑3 (MQ=255)
                                                                                                                                                  tAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTTAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGATATGCGCGTGTTTGTATTAATGTAAATGTGAGTCCTTGTTCCa    <  1:165842/149‑1 (MQ=255)
                                                                                                                                                   gaaaaaGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTg         <  2:10076/142‑1 (MQ=255)
                                                                                                                                                   aaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtccc   >  1:305605‑M2/1‑2 (MQ=255)
                                                                                                                                                   aaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttct         <  2:225003‑M2/143‑142 (MQ=255)
                                                                                                                                                    aggaaggtgcgaacgagtccctgttatgagatcaggtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttctt        >  2:108026‑M2/1‑1 (MQ=255)
                                                                                                                                                    aggaaggtgcgaacaagtccctgatatcagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgagagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccg  >  1:210615‑M2/1‑1 (MQ=255)
                                                                                                                                                    aaaaaGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGt        <  2:59825/143‑1 (MQ=255)
                                                                                                                                                    aaaaaGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAct  >  1:263366/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACT  >  CP009273/2400113‑2400408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.