Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,206,8800TC53.1% 6.0 / 111.0 97pseudogene (12/165 nt)icdCpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base C (29/22);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAA  >  CP009273/1206734‑1207026
                                                                                                                                                  |                                                                                                                                                  
aaaaaTGGTTATCAAAACTGAAAAACACCTGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCatat                                                                                                                                                  <  2:850251/149‑1 (MQ=255)
  aaaTGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATgg                                                                                                                                                <  2:586352/149‑1 (MQ=255)
        ttATCAACACTCAAAAACACGTCTTTATAACCAATGATCAATAGAGATGTTATGAGCTAAGGAGTTAGATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGtt                                                                                                                                             >  1:94978/1‑146 (MQ=255)
        ttATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGa                                                                                                                                          >  2:872693/1‑149 (MQ=255)
        ttATCAAAACTCAAAAACACATGTTGATAATCAATGAGTTATAGAAATGCTAAGTGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTAGGTCAGATAGTTACGATTGAGTCATCTAACTAATGCTGCGCCATATGGGTTGGa                                                                                                                                          >  2:645504/1‑149 (MQ=255)
         tATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAGTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGAc                                                                                                                                         >  2:857847/1‑149 (MQ=255)
          aTCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACt                                                                                                                                        >  2:625697/1‑149 (MQ=255)
          aTCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTgg                                                                                                                                           >  1:981516/1‑146 (MQ=255)
           tCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTg                                                                                                                                       >  2:740583/1‑149 (MQ=255)
            cAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATCGTTACGTTTTAGTCATATAACTAATCCTGCGCCATATGGGTTGGTCTGa                                                                                                                                      >  2:192936/1‑149 (MQ=255)
             aaaaCTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGaa                                                                                                                                     >  2:912123/1‑149 (MQ=255)
                  tCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGACTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCGCATGGGTTGGACCGAAGCGGc                                                                                                                                >  2:797565/1‑149 (MQ=255)
                     aaaaCTCATGTTTATAATCAATGAGGTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTATCAAATGCGGCGCCACATGGGTTGGACCGAAGCGGCTGa                                                                                                                             <  2:278614/149‑1 (MQ=255)
                     aaaaCACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGa                                                                                                                             <  2:909552/149‑1 (MQ=255)
                     aaaaCACATGCTTATAATCAATGAGTTATAGAAATGATAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGc                                                                                                                                <  1:215014/146‑1 (MQ=255)
                      aaaCACATGTTTATAATCAATGAGTTAGAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCt                                                                                                                               <  1:814558/146‑1 (MQ=255)
                        acacATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATACTGCGCCATATGGGTTGGACTGAAGCGCCTGa                                                                                                                             >  1:922476/1‑146 (MQ=255)
                           caTGTTTATAATCAATGAGTTATAGAAATGCTAAGTGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAt                                                                                                                       <  2:1007566/149‑1 (MQ=255)
                            aTGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                                                                      >  2:713705/1‑149 (MQ=255)
                            aTGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                                                                      <  2:340727/149‑1 (MQ=255)
                                 aataAGCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaa                                                                                                                 <  2:42004/148‑1 (MQ=38)
                                     aTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                                                                                >  1:658566/1‑146 (MQ=255)
                                     aTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                                                                                >  1:838036/1‑146 (MQ=255)
                                     aTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaaa                                                                                                                <  1:60122/146‑1 (MQ=38)
                                      tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                                                                               <  1:833476/146‑1 (MQ=255)
                                        aaTGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATg                                                                                                          <  2:965100/149‑1 (MQ=37)
                                         aTGGGTTAAAGAAATGCTAAGGGCGAATGGTTTATATGCAAATTAGTAAAATTATGTTGTTATGTCAGATAGTTACGATTTAGTCATCTAACTAACGCTGCGCCATATGGGTTGGACTGAATCGGCTGACCTGATTGTTAAAGGTa                                                                                                            <  1:361359/146‑1 (MQ=255)
                                          tGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                                                                           <  1:90173/146‑1 (MQ=255)
                                            aGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                                                                                      <  2:931045/149‑1 (MQ=255)
                                            aGTTATAGAAAGTCTAAGGGCTAATGAGTTAAAAGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGTCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg                                                                                                         <  1:559778/146‑1 (MQ=25)
                                               tataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                                                   <  2:542423/149‑1 (MQ=255)
                                                ataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAggg                                                                                                     >  1:832372/1‑146 (MQ=37)
                                                 taGAAATGCTAAGGGCTTATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgc                                                                                                    <  1:843598/146‑1 (MQ=255)
                                                   gAAATGCTAAGGGCTAATGAGTTATATGCACATTCGCTCAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaat                                                                                               >  2:277495/1‑149 (MQ=21)
                                                   gAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcgc                                                                                                  >  1:874064/1‑146 (MQ=255)
                                                    aaaTGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatc                                                                                              >  2:603498/1‑149 (MQ=255)
                                                    aaaTGCTAAAGGCAGATGATTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGca                                                                                                 >  1:909438/1‑146 (MQ=25)
                                                      aTGCTGAGGGCTAATGAGTTATATGCAAATTAGTAGAATTATTTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCTACATGGGTTGGTGCTAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaat                                                                                               <  1:429270/146‑1 (MQ=25)
                                                          tAAGGGCTAATGGGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGcc                                                                                        >  2:213083/1‑149 (MQ=255)
                                                           aaGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATGTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGcaa                                                                                       >  2:111607/1‑147 (MQ=255)
                                                           aaGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaatcaacg                                                                                          <  1:605661/146‑3 (MQ=25)
                                                            aGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                                                                      <  2:789019/149‑1 (MQ=255)
                                                             gggCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGcc                                                                                        <  1:898118/146‑1 (MQ=255)
                                                               gCTAATGAGTTATATGCAAATTAGTAAAATTATGTAGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCAAAAAGGGTTGGACTGAAGCGGCTGACCGGATTGTTAACGGTATGGAAGGCGCAATCAATGCCaa                                                                                      >  1:648749/1‑146 (MQ=38)
                                                               gCTAATGAGTTATATGCAAATTAGTAAAATGGTGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaatcaacgcgaaaac                                                                                   <  2:302998/149‑10 (MQ=18)
                                                                  aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTACGGGTATGGCAGGCGCAATCAACGcggaaaccgt                                                                                >  2:10371/1‑140 (MQ=18)
                                                                  aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGt                                                                                >  2:641690/1‑149 (MQ=18)
                                                                   aTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTa                                                                               >  2:37806/1‑149 (MQ=18)
                                                                        ttATATGCAAATTAGTAAAATTATGTTGGTATGGCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTa                                                                          >  2:614537/1‑149 (MQ=17)
                                                                        ttATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc                                                                             >  1:336346/1‑146 (MQ=18)
                                                                        ttATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc                                                                             >  1:877383/1‑146 (MQ=18)
                                                                              gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                                                       <  1:179247/146‑1 (MQ=255)
                                                                              gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGa                                                                       >  1:882798/1‑146 (MQ=17)
                                                                              gCAAATGAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAGCTGAGTGTTAAACGGATGGAAGGCGCGATCAATGGCAGGACCGCAGCTTCTGa                                                                       >  1:36401/1‑146 (MQ=255)
                                                                               cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc                                                                   <  2:713513/149‑1 (MQ=255)
                                                                               cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAGTCAACGCGCAAACCGTAACCTATGAc                                                                      >  1:331948/1‑146 (MQ=17)
                                                                               cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAGGCGAAAACCGTAACCTATGACttg                                                                   >  2:1082390/1‑148 (MQ=17)
                                                                                aaaTGAGTAAAAGTATGTTGCTATGTCCGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg                                                                  <  2:19121/149‑1 (MQ=14)
                                                                                 aaTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                                                    >  1:354/1‑146 (MQ=255)
                                                                                 aaTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa                                                                 <  2:672376/149‑1 (MQ=14)
                                                                                     aGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt                                                             <  2:915886/149‑1 (MQ=255)
                                                                                      gTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTACTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGCCTTCGagc                                                               >  1:986098/1‑144 (MQ=14)
                                                                                       tAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg                                                              >  1:1009139/1‑146 (MQ=255)
                                                                                        aaaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt                                                             <  1:57483/146‑1 (MQ=255)
                                                                                        aaaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGt                                                             <  1:964512/146‑1 (MQ=14)
                                                                                         aaaTTATATTGCTATAGCATATAGGTACGATTGAAGGATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                            >  1:879673/1‑146 (MQ=14)
                                                                                          aaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGACGCGGCGGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGAt                                                        >  2:444591/1‑149 (MQ=255)
                                                                                          aaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGAt                                                        >  2:420709/1‑149 (MQ=255)
                                                                                              aTGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGat                                                    <  2:460857/149‑2 (MQ=11)
                                                                                               tGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATgg                                                      >  1:437701/1‑146 (MQ=11)
                                                                                                gTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCGACATGGGTTGGACCGAAGCGGCTGAATTAATTGTTAAAGGTATGGAGGGCGCAATCAGCGCGAAAACCGTAACCTATAACTTCGAGCGTCTGGTGGa                                                     >  1:697555/1‑146 (MQ=14)
                                                                                                   gCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGGTGgcgc                                               >  2:869047/1‑149 (MQ=11)
                                                                                                      aTGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGCCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa                                            <  2:71207/149‑1 (MQ=11)
                                                                                                      aTGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTAGGAAAAAGGTGCCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa                                            <  2:268393/149‑1 (MQ=11)
                                                                                                       tGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCt                                              >  1:1040155/1‑146 (MQ=11)
                                                                                                          cAGGTAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa                                           <  1:253118/146‑2 (MQ=11)
                                                                                                          cAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAgctg                                        <  2:603489/149‑1 (MQ=255)
                                                                                                             aTAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGGCTGATGGAGGCGGCTAAActgctg                                     >  2:253416/1‑149 (MQ=11)
                                                                                                              tAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                    <  2:222544/149‑1 (MQ=14)
                                                                                                                 ttACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGa                                    >  1:390793/1‑146 (MQ=255)
                                                                                                                 ttACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                    >  1:907704/1‑146 (MQ=14)
                                                                                                                  tACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa                                   >  1:143605/1‑146 (MQ=14)
                                                                                                                    ccaTTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGtt                              <  2:82524/147‑1 (MQ=255)
                                                                                                                    cGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGtt                              <  2:372316/149‑1 (MQ=255)
                                                                                                                      aTTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAGTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCa                            <  2:421047/149‑1 (MQ=255)
                                                                                                                      aTTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                            <  2:357741/149‑1 (MQ=17)
                                                                                                                       tttAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTTAACTGCTGAAATGtt                              <  1:573897/146‑1 (MQ=14)
                                                                                                                       tttAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt                              >  1:869022/1‑146 (MQ=17)
                                                                                                                        ttAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATACCAAGACCGTAACTTATGACTTCGAAGGTCTGGTGGAAGGCGCTAAGCTGCTGAAATGTTCaga                          >  2:667561/1‑149 (MQ=255)
                                                                                                                        ttAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                          <  2:196977/149‑1 (MQ=17)
                                                                                                                             cATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTg                     <  2:667111/149‑1 (MQ=255)
                                                                                                                                   actaaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAg               >  2:1102964/1‑149 (MQ=255)
                                                                                                                                   actaaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGATTGCTg                  >  1:215473/1‑146 (MQ=255)
                                                                                                                                   actaaTGCTGCCGCATATGGTTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAg               <  2:1005116/149‑1 (MQ=255)
                                                                                                                                      aaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAg               <  1:562741/146‑1 (MQ=255)
                                                                                                                                         gctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGTGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatca         >  2:789657/1‑149 (MQ=255)
                                                                                                                                             cgcCATCTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAAGTTCAGAGTTTGGTGAAGCGatcat        >  1:496365/1‑146 (MQ=255)
                                                                                                                                                cATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa  <  2:253411/149‑1 (MQ=255)
                                                                                                                                                 atatGGGTTGGACTGAAGGGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa    <  1:194477/146‑1 (MQ=255)
                                                                                                                                                 atatGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa    >  1:934223/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAAATGGTTATCAAAACTCAAAAACACATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAA  >  CP009273/1206734‑1207026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: