Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,206,9070GA43.2% 82.0 / 109.8 95pseudogene (39/165 nt)icdCpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion
Reads supporting (aligned to +/- strand):  ref base G (29/25);  new base A (23/18);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTG  >  CP009273/1206761‑1207055
                                                                                                                                                  |                                                                                                                                                    
caTGTTTATAATCAATGAGTTATAGAAATGCTAAGTGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAt                                                                                                                                                    <  2:1007566/149‑1 (MQ=255)
 aTGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                                                                                                   <  2:340727/149‑1 (MQ=255)
 aTGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                                                                                                                   >  2:713705/1‑149 (MQ=255)
      aataAGCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaa                                                                                                                                              <  2:42004/148‑1 (MQ=38)
          aTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                                                                                                             >  1:658566/1‑146 (MQ=255)
          aTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                                                                                                             >  1:838036/1‑146 (MQ=255)
          aTCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTaaa                                                                                                                                             <  1:60122/146‑1 (MQ=38)
           tCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                                                                                                            <  1:833476/146‑1 (MQ=255)
             aaTGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATg                                                                                                                                       <  2:965100/149‑1 (MQ=37)
              aTGGGTTAAAGAAATGCTAAGGGCGAATGGTTTATATGCAAATTAGTAAAATTATGTTGTTATGTCAGATAGTTACGATTTAGTCATCTAACTAACGCTGCGCCATATGGGTTGGACTGAATCGGCTGACCTGATTGTTAAAGGTa                                                                                                                                         <  1:361359/146‑1 (MQ=255)
               tGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                                                                                                        <  1:90173/146‑1 (MQ=255)
                 aGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                                                                                                                   <  2:931045/149‑1 (MQ=255)
                 aGTTATAGAAAGTCTAAGGGCTAATGAGTTAAAAGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGTCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg                                                                                                                                      <  1:559778/146‑1 (MQ=25)
                    tataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                                                                                                <  2:542423/149‑1 (MQ=255)
                     ataGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAggg                                                                                                                                  >  1:832372/1‑146 (MQ=37)
                      taGAAATGCTAAGGGCTTATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgc                                                                                                                                 <  1:843598/146‑1 (MQ=255)
                        gAAATGCTAAGGGCTAATGAGTTATATGCACATTCGCTCAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaat                                                                                                                            >  2:277495/1‑149 (MQ=21)
                        gAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcgc                                                                                                                               >  1:874064/1‑146 (MQ=255)
                         aaaTGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGcaatc                                                                                                                           >  2:603498/1‑149 (MQ=255)
                         aaaTGCTAAAGGCAGATGATTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGca                                                                                                                              >  1:909438/1‑146 (MQ=25)
                           aTGCTGAGGGCTAATGAGTTATATGCAAATTAGTAGAATTATTTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCTACATGGGTTGGTGCTAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaat                                                                                                                            <  1:429270/146‑1 (MQ=25)
                               tAAGGGCTAATGGGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGcc                                                                                                                     >  2:213083/1‑149 (MQ=255)
                                aaGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATGTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGcaa                                                                                                                    >  2:111607/1‑147 (MQ=255)
                                aaGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaatcaacg                                                                                                                       <  1:605661/146‑3 (MQ=25)
                                 aGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                                                                                                   <  2:789019/149‑1 (MQ=255)
                                  gggCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGcc                                                                                                                     <  1:898118/146‑1 (MQ=255)
                                    gCTAATGAGTTATATGCAAATTAGTAAAATTATGTAGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCAAAAAGGGTTGGACTGAAGCGGCTGACCGGATTGTTAACGGTATGGAAGGCGCAATCAATGCCaa                                                                                                                   >  1:648749/1‑146 (MQ=38)
                                    gCTAATGAGTTATATGCAAATTAGTAAAATGGTGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGcaatcaacgcgaaaac                                                                                                                <  2:302998/149‑10 (MQ=18)
                                       aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTACGGGTATGGCAGGCGCAATCAACGcggaaaccgt                                                                                                             >  2:10371/1‑140 (MQ=18)
                                       aaTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGt                                                                                                             >  2:641690/1‑149 (MQ=18)
                                        aTGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTa                                                                                                            >  2:37806/1‑149 (MQ=18)
                                             ttATATGCAAATTAGTAAAATTATGTTGGTATGGCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTa                                                                                                       >  2:614537/1‑149 (MQ=17)
                                             ttATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc                                                                                                          >  1:336346/1‑146 (MQ=18)
                                             ttATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAc                                                                                                          >  1:877383/1‑146 (MQ=18)
                                                   gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                                                                                                    <  1:179247/146‑1 (MQ=255)
                                                   gCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGa                                                                                                    >  1:882798/1‑146 (MQ=17)
                                                   gCAAATGAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAGCTGAGTGTTAAACGGATGGAAGGCGCGATCAATGGCAGGACCGCAGCTTCTGa                                                                                                    >  1:36401/1‑146 (MQ=255)
                                                    cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc                                                                                                <  2:713513/149‑1 (MQ=255)
                                                    cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAGTCAACGCGCAAACCGTAACCTATGAc                                                                                                   >  1:331948/1‑146 (MQ=17)
                                                    cAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAGGCGAAAACCGTAACCTATGACttg                                                                                                >  2:1082390/1‑148 (MQ=17)
                                                     aaaTGAGTAAAAGTATGTTGCTATGTCCGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg                                                                                               <  2:19121/149‑1 (MQ=14)
                                                      aaTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGCTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                                                                                                 >  1:354/1‑146 (MQ=255)
                                                      aaTTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa                                                                                              <  2:672376/149‑1 (MQ=14)
                                                          aGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt                                                                                          <  2:915886/149‑1 (MQ=255)
                                                           gTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTACTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGCCTTCGagc                                                                                            >  1:986098/1‑144 (MQ=14)
                                                            tAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg                                                                                           >  1:1009139/1‑146 (MQ=255)
                                                             aaaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt                                                                                          <  1:57483/146‑1 (MQ=255)
                                                             aaaaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGt                                                                                          <  1:964512/146‑1 (MQ=14)
                                                              aaaTTATATTGCTATAGCATATAGGTACGATTGAAGGATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc                                                                                         >  1:879673/1‑146 (MQ=14)
                                                               aaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGACGCGGCGGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGAt                                                                                     >  2:444591/1‑149 (MQ=255)
                                                               aaTTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGAt                                                                                     >  2:420709/1‑149 (MQ=255)
                                                                   aTGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGat                                                                                 <  2:460857/149‑2 (MQ=11)
                                                                    tGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATgg                                                                                   >  1:437701/1‑146 (MQ=11)
                                                                     gTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCGACATGGGTTGGACCGAAGCGGCTGAATTAATTGTTAAAGGTATGGAGGGCGCAATCAGCGCGAAAACCGTAACCTATAACTTCGAGCGTCTGGTGGa                                                                                  >  1:697555/1‑146 (MQ=14)
                                                                        gCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGGTGgcgc                                                                            >  2:869047/1‑149 (MQ=11)
                                                                           aTGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGCCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa                                                                         <  2:71207/149‑1 (MQ=11)
                                                                           aTGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTAGGAAAAAGGTGCCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaa                                                                         <  2:268393/149‑1 (MQ=11)
                                                                            tGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCt                                                                           >  1:1040155/1‑146 (MQ=11)
                                                                               cAGGTAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa                                                                        <  1:253118/146‑2 (MQ=11)
                                                                               cAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAgctg                                                                     <  2:603489/149‑1 (MQ=255)
                                                                                  aTAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGGCTGATGGAGGCGGCTAAActgctg                                                                  >  2:253416/1‑149 (MQ=11)
                                                                                   tAGTTACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                                                 <  2:222544/149‑1 (MQ=14)
                                                                                      ttACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGa                                                                 >  1:390793/1‑146 (MQ=255)
                                                                                      ttACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa                                                                 >  1:907704/1‑146 (MQ=14)
                                                                                       tACGATTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa                                                                >  1:143605/1‑146 (MQ=14)
                                                                                         ccaTTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGtt                                                           <  2:82524/147‑1 (MQ=255)
                                                                                         cGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGtt                                                           <  2:372316/149‑1 (MQ=255)
                                                                                           aTTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAGTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCa                                                         <  2:421047/149‑1 (MQ=255)
                                                                                           aTTTAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                                                         <  2:357741/149‑1 (MQ=17)
                                                                                            tttAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTTAACTGCTGAAATGtt                                                           <  1:573897/146‑1 (MQ=14)
                                                                                            tttAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt                                                           >  1:869022/1‑146 (MQ=17)
                                                                                             ttAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATACCAAGACCGTAACTTATGACTTCGAAGGTCTGGTGGAAGGCGCTAAGCTGCTGAAATGTTCaga                                                       >  2:667561/1‑149 (MQ=255)
                                                                                             ttAGTCATCTAACTAATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                       <  2:196977/149‑1 (MQ=17)
                                                                                                  cATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTg                                                  <  2:667111/149‑1 (MQ=255)
                                                                                                        actaaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAg                                            >  2:1102964/1‑149 (MQ=255)
                                                                                                        actaaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGATTGCTg                                               >  1:215473/1‑146 (MQ=255)
                                                                                                        actaaTGCTGCCGCATATGGTTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAg                                            <  2:1005116/149‑1 (MQ=255)
                                                                                                           aaTGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAg                                            <  1:562741/146‑1 (MQ=255)
                                                                                                              gctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGTGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatca                                      >  2:789657/1‑149 (MQ=255)
                                                                                                                  cgcCATCTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAAGTTCAGAGTTTGGTGAAGCGatcat                                     >  1:496365/1‑146 (MQ=255)
                                                                                                                     cATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa                               <  2:253411/149‑1 (MQ=255)
                                                                                                                      atatGGGTTGGACTGAAGGGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa                                 <  1:194477/146‑1 (MQ=255)
                                                                                                                      atatGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaa                                 >  1:934223/1‑146 (MQ=255)
                                                                                                                           ggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTa                         >  2:704303/1‑149 (MQ=255)
                                                                                                                             ttGGACTGAAGCGGCTGACCTGATTGTTAAAGGCATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAAt                       >  2:736520/1‑149 (MQ=255)
                                                                                                                                   tGAAGCGGCTGACCTGATTGTTAAAGGTCTGGAAGGCGCAATCAATGCCAAGACCGTAACTTAGGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGACGTTGGTGAAGCGATCATCGAAAACATGTAATCTCTcc                 >  2:748319/1‑149 (MQ=255)
                                                                                                                                   tGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAAtctc                    >  1:164447/1‑146 (MQ=255)
                                                                                                                                   tGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAAtctc                    >  1:895089/1‑146 (MQ=255)
                                                                                                                                   tGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTcc                 >  2:632361/1‑149 (MQ=255)
                                                                                                                                     aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAATTTTGACTTCGAACGTCTGACGGAAGTCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGGGATCATCGAAAACATGTAATCTCTCCAt               >  2:273140/1‑149 (MQ=18)
                                                                                                                                        gggttGACGTGATTGTTAAAGGTAGGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGGCTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGGGTTTGGTGAAGCGATCATCGAAAACGTGTGATCTCTCCAt               <  1:640795/142‑1 (MQ=255)
                                                                                                                                        cGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCAt               <  1:542032/146‑1 (MQ=255)
                                                                                                                                        cGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGCCTGATGGAAGGCGCTAAGATGCTGAAATGTTCCAGGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTccgt               >  1:337172/1‑144 (MQ=18)
                                                                                                                                                cTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTaaa       >  1:803334/1‑146 (MQ=255)
                                                                                                                                                cTGATCGTTCAAGGTATGGAAGGCGCAACCAATGCCAAGACCGTAACTTATGACCTCGAACGTCTGCAGGAAGGCGCTAAGCTGCTGCAATGTTCAGAGTTTGGTGCAGCGATCATCGAACACATGTAATCTCTCCATGTGTTgaa       >  1:77391/1‑146 (MQ=255)
                                                                                                                                                  gATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTCATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTg  <  2:505/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CATGTTTATAATCAATGAGTTATAGAAATGCTAAGGGCTAATGAGTTATATGCAAATTAGTAAAATTATGTTGCTATGTCAGATAGTTACGATTTAGTCATCTAACTAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTG  >  CP009273/1206761‑1207055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑