Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,969,337 G→T E278* (GAG→TAG)  wecE → TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,969,3370GT98.7% 229.9 / NA 77E278* (GAG→TAG) wecETDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (37/39);  minor base A (0/1);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTG  >  CP009273/3969196‑3969477
                                                                                                                                             |                                                                                                                                            
tCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAg                                                                                                                                            >  1:482056/1‑144 (MQ=255)
tCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGCCGAAAGCCGGGCGTATCTAGCTGcc                                                                                                                                       <  2:521264/149‑1 (MQ=255)
  cAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAGCTGGCAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTAGACCTGCGACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGt                                                                                                                                     >  2:751742/1‑149 (MQ=255)
  cAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGGGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCt                                                                                                                                          <  1:740124/144‑1 (MQ=255)
     cTATTTGATGTCCGATCTGCAAGCTGCATCCCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGa                                                                                                                                  <  2:425837/149‑1 (MQ=255)
     cTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGcc                                                                                                                                       <  1:826724/144‑1 (MQ=255)
      tATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCTCTGTGGCAAACCTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCg                                                                                                                                      <  1:243041/144‑1 (MQ=255)
      tATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTCCCg                                                                                                                                      >  1:209880/1‑144 (MQ=255)
        tttGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTc                                                                                                                               >  2:515837/1‑149 (MQ=255)
        tttGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTc                                                                                                                               >  2:77231/1‑149 (MQ=255)
           gATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGGCGATTCCCg                                                                                                                            >  2:485458/1‑149 (MQ=255)
               tCCGAGCTGCAAGCTGCAGTCCTTTGGGCGCAACTGGACGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTccc                                                                                                                             <  1:857403/144‑1 (MQ=255)
                 gcaTCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAACACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGa                                                                                                                           <  1:869243/142‑1 (MQ=255)
                 cGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTGTCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGGAAGCCGGGCGTCTCTAGCTGCCGTCGGTTTCCGa                                                                                                                           >  1:591210/1‑144 (MQ=255)
                   aTCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTTCGAAGCGTTGTCGCCTCTGTCTAAAGCCGGGTGTATCTAGCTGCCGTCGATTCCCGATGGCTGc                                                                                                                    >  2:40220/1‑149 (MQ=255)
                   aTCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGc                                                                                                                    >  2:175293/1‑149 (MQ=255)
                     cTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGc                                                                                                                       <  1:389948/144‑1 (MQ=255)
                            cTGCCTACCTGGGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAACCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGc                                                                                                                <  1:710244/144‑1 (MQ=255)
                               cATACCTGTGGGCGCAACTGGAAGCAACGGATCGTATCAACCAGCAACGTCGGGCGCTGTGGCAAAACTACTACGCTGCGTTAGGGCCGCTGGCGAAAGCCGGGCGTAGCTAGCTGCCGTCGATTCCCGAGGGCTGCGGGCAGa                                                                                                             >  1:415453/1‑144 (MQ=255)
                                aTACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACgcgc                                                                                                       <  2:15265/149‑1 (MQ=255)
                                  aCCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAAcg                                                                                                          >  1:661634/1‑144 (MQ=255)
                                     tgtgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACgcgc                                                                                                       <  1:80046/144‑1 (MQ=255)
                                     tgtgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACgcgc                                                                                                       <  1:435484/144‑1 (MQ=255)
                                     tgtgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACgcgc                                                                                                       <  1:542474/144‑1 (MQ=255)
                                      gtgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGCCTGCGTGCAGAACGCGCa                                                                                                      >  1:713287/1‑144 (MQ=255)
                                             cAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTc                                                                                               >  1:631488/1‑144 (MQ=255)
                                              aaCTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTCCTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCt                                                                                              >  1:269675/1‑144 (MQ=255)
                                              aaCTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAAGGCGCATATGTTCAACAtt                                                                                         >  2:102870/1‑149 (MQ=255)
                                                 tGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACa                                                                                           >  1:321394/1‑144 (MQ=255)
                                                 gggAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCCGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGAGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGTGCATATGTTCTACa                                                                                           <  1:576155/143‑1 (MQ=255)
                                                   gAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTGCTACAtt                                                                                         >  1:237355/1‑144 (MQ=255)
                                                    aaGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTg                                                                                   >  2:773309/1‑149 (MQ=255)
                                                           ggATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCg                                                                                 <  1:902636/144‑1 (MQ=255)
                                                            gATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGACTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGCACGCGCATATGTTCTCCCTTGAACTGCga                                                                                >  1:887617/1‑143 (MQ=255)
                                                                 tATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGatat                                                                           >  1:96472/1‑144 (MQ=255)
                                                                 aaacAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGatat                                                                           >  1:297136/4‑144 (MQ=255)
                                                                   tCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGAc                                                                    >  2:105643/1‑149 (MQ=255)
                                                                     aaCCAGCCACGGCTGGCGCTGTGGCAAAACTACTACGTTGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCTGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATtgat                                                                       <  1:350484/144‑1 (MQ=255)
                                                                      aCCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCgg                                                                 >  2:40811/1‑149 (MQ=255)
                                                                       ccAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGa                                                                <  2:924694/149‑1 (MQ=255)
                                                                             aCGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAg                                                               <  1:207440/144‑1 (MQ=255)
                                                                                tCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGa                                                       >  2:914571/1‑149 (MQ=255)
                                                                                  tGGCGCTGTGGCAAAACTGCTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAATCTGCGGGATATTGATGACCGGAGCGCGTTGAtt                                                     <  2:659972/149‑1 (MQ=255)
                                                                                    ttccgatctgaaaaCTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTaa                                                   <  2:229324/139‑1 (MQ=255)
                                                                                     cgcTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGa                                                       <  1:845649/144‑1 (MQ=255)
                                                                                         gtgGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTaa                                                   <  1:69975/144‑1 (MQ=255)
                                                                                         gtgGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTaa                                                   <  1:573619/144‑1 (MQ=255)
                                                                                         gtgGCAAAACTACTACGAGGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTaa                                                   <  1:42529/144‑1 (MQ=255)
                                                                                          tgGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACGTTATACTGCGGGATATTGATGACCGGGGCGGGTTGATTAGCTTTCt                                             >  2:616472/1‑149 (MQ=255)
                                                                                          tgGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCt                                             <  2:44771/149‑1 (MQ=255)
                                                                                          tgGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCt                                             >  2:166552/1‑149 (MQ=255)
                                                                                          cggCAAAACTGCTACGAGGCGGTGGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCCGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAgagtgctcaggtgc                                                  <  1:8898/143‑14 (MQ=255)
                                                                                            gCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACtt                                                >  1:484402/1‑144 (MQ=255)
                                                                                             caaacctccgccggtGCGTTGCCGCCGCTGGCGAAAGCCGGTGGTATCTAGCTGCCGGCGAGTCCCGATGGCTGCGTGCAGATCGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACttt                                               <  1:815776/130‑1 (MQ=255)
                                                                                             cAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACttt                                               <  1:179236/144‑1 (MQ=255)
                                                                                              aaacCTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAAcagat                                              >  1:208658/1‑140 (MQ=255)
                                                                                              aaaaCTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGACGTCGATTCCCGATGGCTGCGTGCAGAACGAGCATATGTTCTACATTAAACTCCGGAGTATTGTAGACCGGCGCGAGTCGATTAACTTTc                                              >  1:244791/1‑144 (MQ=255)
                                                                                                      tacGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGa                                 >  2:909078/1‑149 (MQ=255)
                                                                                                        cGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGAGATTGATGACCGGAGCGCGTTGGTTCACTTTCTGAAAGAAGc                                    >  1:161224/1‑144 (MQ=255)
                                                                                                          aTGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCgg                                  >  1:444248/1‑144 (MQ=255)
                                                                                                             cGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATg                          <  2:291458/149‑1 (MQ=255)
                                                                                                             cGTTAGCGCCGCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATg                          <  2:687162/149‑1 (MQ=255)
                                                                                                              gTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTTTCTTCCCGCGATTCCCGAGGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAAt                              >  1:204964/1‑144 (MQ=255)
                                                                                                              gTTAGCGCCTCTGGCGAAAACCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAGGCGGaaaa                              >  1:59161/1‑143 (MQ=255)
                                                                                                               ttAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGc                        <  2:37565/149‑1 (MQ=255)
                                                                                                               ttAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGGCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGAGTAACTTTCTGAAAGAAGCGGAAATCATGGc                        <  2:540008/149‑1 (MQ=255)
                                                                                                                 aGCGCCTCTGGGGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCAt                           <  1:348255/144‑1 (MQ=255)
                                                                                                                    gcCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGc                        <  1:804683/144‑1 (MQ=255)
                                                                                                                         tGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCAt              >  2:27075/1‑149 (MQ=255)
                                                                                                                           gCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTa            >  2:724939/1‑149 (MQ=255)
                                                                                                                             gAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACa          <  2:994217/149‑1 (MQ=255)
                                                                                                                               aaGCCGGGCGTATCAAGCTGCCGTCGATTCCCGATGGCTGCGTGTAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAACCGGAAATCATGGCGGTGTTTCATTACAtt        <  2:727721/149‑1 (MQ=255)
                                                                                                                                aggcGGGCGTACCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTa            <  1:628195/141‑1 (MQ=255)
                                                                                                                                aGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTGCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTa            <  1:781809/144‑1 (MQ=255)
                                                                                                                                   cGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTTGAAGAAGCGGAAATCATGGCGGTGTTTCATTACAt         <  1:667418/144‑1 (MQ=255)
                                                                                                                                    gggCGTAGCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTATACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCt   <  2:879028/149‑1 (MQ=255)
                                                                                                                                     ggCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGTCTGGAGCAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTg  >  2:751977/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTG  >  CP009273/3969196‑3969477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: