Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 704,377 C→A N196K (AAC→AAA)  chiP → chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273704,3770CA98.2% 175.3 / NA 55N196K (AAC→AAA) chiPchitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (35/19);  total (36/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAA  >  CP009273/704242‑704521
                                                                                                                                       |                                                                                                                                                
cATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACaa                                                                                                                                          <  1:93214/144‑1 (MQ=255)
 aTTCAGCCCACTCGCCAAAGGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTATTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACaaa                                                                                                                                         >  1:343777/1‑144 (MQ=255)
       ccAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCg                                                                                                                                   >  1:141209/1‑144 (MQ=255)
        tcaCTGGTCAAACGCTGTTAGCTCCGCACTGGAGCTGTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGt                                                                                                                                  <  1:785044/142‑1 (MQ=255)
                cAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTg                                                                                                                          <  1:514946/144‑1 (MQ=255)
                  aaCGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGa                                                                                                                        >  1:81172/1‑144 (MQ=255)
                   aCGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGa                                                                                                                  >  2:401388/1‑149 (MQ=255)
                   aCGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAACGCCGTGGCATCTGGAAATGGa                                                                                                                  >  2:490275/1‑149 (MQ=255)
                     gCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGTCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGATGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAAt                                                                                                                     >  1:704712/1‑144 (MQ=255)
                             cgccgcACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGt                                                                                                             >  1:352168/1‑144 (MQ=255)
                                   aCTGGGGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGACTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATc                                                                                                       <  1:25750/144‑1 (MQ=255)
                                        aGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAAc                                                                                                  >  1:889759/1‑144 (MQ=255)
                                         gCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGAACAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATaaa                                                                                            <  2:15150/149‑1 (MQ=255)
                                          cTTTATGCCAGGTACTTATCAGGGCGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATCTGGACCAAAGAATACAAAGCGCCGTGGCCTCTGGCAATGGATGAGTTCTATCAGAACGa                                                                                                <  1:694261/144‑1 (MQ=255)
                                                  cAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTGCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTGATCAGAACGATAAAacac                                                                                        >  1:269746/1‑142 (MQ=255)
                                                   aGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCTAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAaccac                                                                                       >  1:453580/1‑144 (MQ=255)
                                                    ggTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAaccacc                                                                                      <  1:945158/144‑1 (MQ=255)
                                                           aTCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTAt                                                                          >  2:979401/1‑149 (MQ=255)
                                                             cAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCt                                                                        <  2:55086/149‑1 (MQ=255)
                                                              aGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCACAGAATACAAAGCGCCGTGGCATCGGGAAATGGATGAGTTTTATCAGAACGATAGAACCACCAAAGGTGAtg                                                                            >  1:639929/1‑143 (MQ=255)
                                                                ggggCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCa                                                                     >  2:405019/1‑149 (MQ=255)
                                                                ggggCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCa                                                                     >  2:269440/1‑149 (MQ=255)
                                                                ggcgCGGAAGCCGGGGCGAATGTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGCATACAAAGCGCCGTGGCATATGGAAATGGATGAGTTTTATCAGAACGATAAACACACCAAAGTGGATTac                                                                          >  1:715796/4‑143 (MQ=255)
                                                                   tcGGAAGCCGGGGCGAATTTTGATTACGGCGATGCCTGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTg                                                                       <  1:247563/143‑1 (MQ=255)
                                                                      gAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTATCCTGAATGTGGAGAAAAGAAGGCAAAGGGGCGCGGCTGGTACTCAACCACGAGTTGTAGCAGAACTATAAAACCTCCAAAGTTGGTTATCTGCAc                                                                    >  1:909291/1‑144 (MQ=255)
                                                                        aGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTg                                                             <  2:154976/149‑1 (MQ=255)
                                                                         gCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCtttag                                                            >  2:272847/1‑147 (MQ=255)
                                                                             gggCGAATTTTGATGACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTg                                                             <  1:796860/144‑1 (MQ=255)
                                                                                 gAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGaact                                                    >  2:36641/1‑147 (MQ=255)
                                                                                  aaTTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTCGGGCg                                                        >  1:775347/1‑144 (MQ=255)
                                                                                    ttttGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACg                                                 >  2:788450/1‑149 (MQ=255)
                                                                                            aCGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCaaa                                         >  2:350331/1‑149 (MQ=255)
                                                                                            aCGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCaaa                                         >  2:350360/1‑149 (MQ=255)
                                                                                                   tGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCaaaaa                                       <  1:248011/144‑1 (MQ=255)
                                                                                                        gTGCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTa                             <  2:740172/149‑1 (MQ=255)
                                                                                                          gCGTTGAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTc                                <  1:648623/144‑1 (MQ=255)
                                                                                                               gAGTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAg                      >  2:914906/1‑149 (MQ=255)
                                                                                                                aGTTTCTCCTACATGGGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAACCCCCCAAAGCTGCTTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTg                          <  1:4334/144‑1 (MQ=255)
                                                                                                                 gTTTCTCCTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTCATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCg                    <  2:687997/149‑1 (MQ=255)
                                                                                                                 gTTTCCCCTACATTTGGACCAAAGAATTCAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCg                    <  2:2819/149‑1 (MQ=255)
                                                                                                                  tttCTCCTACATGTGGACCAAAGGATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGATGATTATCTGCATTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGa                        >  1:643535/1‑144 (MQ=255)
                                                                                                                    tctcCTACGTGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAACCCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAg                      <  1:156168/144‑1 (MQ=255)
                                                                                                                     ctcCGAGATGTAGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATATGCAATACTTTGGGGCGTAATACGACTGAAAAAATAACTTCGTACTGGAAGc                     >  1:475671/1‑144 (MQ=255)
                                                                                                                      cccTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCg                    >  1:587621/2‑144 (MQ=255)
                                                                                                                       ccTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATGATCTGCACTCCTTTGGGGCGAAAAACGACTTCAAAAATAACTTCGTACTGGAAGCGGCAttt              >  2:701668/1‑149 (MQ=255)
                                                                                                                        cTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAAGGATAAAACCACTATTTGATATTATCTGCACTCCTTTGGGGCGAAATACGACTTCCAAAATAACTTCGTACTGGAAGCGGCATTTg             >  2:1014859/1‑149 (MQ=255)
                                                                                                                        cTACATGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTg             <  2:621087/149‑1 (MQ=255)
                                                                                                                            aTGTGGACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCa         >  2:828005/1‑149 (MQ=255)
                                                                                                                                ggACCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCg     >  2:999265/1‑149 (MQ=255)
                                                                                                                                 gACCAAAGAATACAAAGCGCCGTGGCAACTGGAAATAGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCa         >  1:409862/1‑144 (MQ=255)
                                                                                                                                  aCCAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAg        >  1:721220/1‑144 (MQ=255)
                                                                                                                                   ccAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTTGAAGCGGCATTTGGTCAgg       >  1:666285/1‑144 (MQ=255)
                                                                                                                                   ccAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGaa  >  2:678749/1‑149 (MQ=255)
                                                                                                                                    cAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGc      >  1:949407/1‑144 (MQ=255)
                                                                                                                                    cAAAGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATAAAACCACCACAGTGGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAGCTTCGTACTGGAAGCGGCATTGGGGCAGGc      >  1:875104/1‑144 (MQ=255)
                                                                                                                                       |                                                                                                                                                
CATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCGAAATACGACTTCAAAAATAACTTCGTACTGGAAGCGGCATTTGGTCAGGCGGAA  >  CP009273/704242‑704521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑