| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 966,112 | A→T | 12.2% | intergenic (+35/‑17) | ycaQ → / → ycaR | DUF1006 family protein with C‑terminal wHTH domain/peroxide and acid resistance protein, UPF0434 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 966,112 | 0 | A | T | 12.2% | 77.7 / 4.1 | 35 | intergenic (+35/‑17) | ycaQ/ycaR | DUF1006 family protein with C‑terminal wHTH domain/peroxide and acid resistance protein, UPF0434 family |
| Reads supporting (aligned to +/- strand): ref base A (18/12); new base T (2/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01 | |||||||||||
GCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGAT > CP009273/965964‑966248 | gCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCCTAACCCGa > 1:66144/1‑149 (MQ=255) aTTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGCTGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGGTTTATGCATTCATAACCCGATCCa > 2:266350/1‑143 (MQ=255) aTTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCa > 2:443952/1‑143 (MQ=255) tttCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGTTCCATCTggag > 1:263588/1‑149 (MQ=255) tttCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGTTCCATCTggag > 1:263699/1‑149 (MQ=255) tCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGCTGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGCTAAGATTTATGCATTCATAACCCGATCCATCt > 2:334251/1‑143 (MQ=255) gCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTgg < 2:179246/143‑1 (MQ=255) tAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGTACCGGCGGGGAAATAGACCCCGTCGCATATATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTggag < 2:370989/143‑1 (MQ=255) tGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGa < 1:328463/149‑1 (MQ=255) ggagGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATAATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATgg < 2:21113/141‑1 (MQ=255) agGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCGTTCATAACCCGATCCATCTGGAGGAACTATGGag > 2:108747/1‑142 (MQ=255) aaCGCGGGTGACATTAGGATGCTGCACGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCACGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACACGATCCATCTGGAGGAAGGATGGATCATCGACTGc > 1:266164/1‑149 (MQ=255) cgcgGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTCTGATAAGATTTATGCATTAATAACCCGATCGATCTGGCGGAGCTATGGACCATCGt > 2:100087/1‑143 (MQ=255) ggTGACATTAGGATGCCGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGaaa < 1:476617/149‑1 (MQ=255) ctgcCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCgg < 1:142041/149‑1 (MQ=255) tgcCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGAGCATCGTCTGCTTGAAATCATtgcctg > 2:468584/1‑143 (MQ=255) aaGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATGTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAAc < 1:369885/149‑1 (MQ=255) aGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACg < 1:195384/149‑1 (MQ=255) aTTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACtt > 2:171119/1‑143 (MQ=255) gCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGtat > 2:40888/1‑143 (MQ=255) ggCTGGGTAATCGACCCCGTCGCATAAATAATTATGACAACACTTATGCATTCATACCCCGATCCATCTGCAGGAACTCTGTATCCTCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCa < 2:465934/143‑1 (MQ=255) gggAAATAGACCCCGTCGCATAAATGATTATGCTAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATACCCAGGAAAAACaa < 1:360233/149‑1 (MQ=255) aGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTa > 1:320460/1‑149 (MQ=255) aCCCCGTCGCCTAAATGAATATGATTAGATTTATGCATTCACACCCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAagag < 2:340734/143‑1 (MQ=255) cccGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCTTTGCCTGCCTGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTg > 1:283358/1‑149 (MQ=255) cccGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTg > 1:271285/1‑149 (MQ=255) tCGGATAGATGATTAAGATAAGAATTAAAAAATAATAACCCGTTCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAAACAATGCCTGCCCGGTTTGCAACGGAAAACTTTGAAAAAAAAAAAAAAAACAAGAGCTTAtt < 2:231750/143‑1 (MQ=255) tCGCATAAATGATGATGATAATATTTATGCATTCATAACCCGTTCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTAtt < 2:231845/143‑1 (MQ=255) cATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTg > 1:250174/1‑149 (MQ=255) tAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCaa > 2:356004/1‑143 (MQ=255) tAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCATATTTGCAAACTGGa > 1:74537/1‑149 (MQ=255) gATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTgg > 2:396881/1‑143 (MQ=255) gATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAAATTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTgg > 2:49335/1‑143 (MQ=255) gATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAAcc < 2:132372/143‑1 (MQ=255) aTTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTGTAACCAGGAAATACAAGAGCTTATTTGCAAACTGAGCAACCTAGCTTTCTCGCTGAGTAAt > 1:67075/1‑149 (MQ=255) cgtAACCCGGTCCATCTGGAGGAACTATGGATAATCGTCTGCTTGAAAGCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGAAAAACAAAAAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGt < 2:138748/141‑1 (MQ=255) | GCGATTACTGATTTCGCTAACTGGCAGCAGGCAACGCGGGTGACATTAGGATGCTGCCCGCAAGGCCTCTTTACTGATTGCCGCACCGGCTGGGAAATAGACCCCGTCGCATAAATGATTATGATAAGATTTATGCATTCATAACCCGATCCATCTGGAGGAACTATGGATCATCGTCTGCTTGAAATCATTGCCTGCCCGGTTTGCAACGGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGAT > CP009273/965964‑966248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |