Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,113,470 C→T 10.5% R305R (CGG→CGA)  cytR ← Anti‑activator for CytR‑CRP nucleoside utilization regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,113,4700CT10.5% 86.9 / 2.4 38R305R (CGG→CGA) cytRAnti‑activator for CytR‑CRP nucleoside utilization regulon
Reads supporting (aligned to +/- strand):  ref base C (15/19);  new base T (2/2);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

CTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCA  >  CP009273/4113325‑4113618
                                                                                                                                                 |                                                                                                                                                    
cTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTACCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCccga                                                                                                                                                   >  1:434847/1‑149 (MQ=255)
     aCCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGAt                                                                                                                                              <  1:517501/149‑1 (MQ=255)
      ccAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGAtt                                                                                                                                             <  1:326603/149‑1 (MQ=255)
              ggCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGGTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAAcg                                                                                                                                     <  1:8095/149‑1 (MQ=255)
              ggCAGTCTTAAAGGTTTACTTTAAAGTAACGCGCGTGTTGATCCCCGGCTGATAAATACGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGAGCAAGCAATAACAGCATAGCTTCCCGACCGAttcc                                                                                                                                           >  2:199151/1‑141 (MQ=255)
                  gTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTaa                                                                                                                                       <  2:128296/143‑1 (MQ=255)
                   tCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCTCGACCGATTTCGTAACGCGGCt                                                                                                                                >  1:54933/1‑149 (MQ=255)
                   tCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCTCGACCGATTTCGTAACGCGGCt                                                                                                                                >  1:54938/1‑149 (MQ=255)
                   tCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAAc                                                                                                                                      >  2:401039/1‑143 (MQ=255)
                      tAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGCCCGACTTCGTAAcgcg                                                                                                                                   >  2:5126/1‑143 (MQ=255)
                      tAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCCCAGTCCGTTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGCTTTCGTAACGCGGCTgcg                                                                                                                             >  1:281038/1‑149 (MQ=255)
                          ggTCTACTTTAAGGTAACGCGCGTGTGGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGAt                                                                                                                         <  1:150177/149‑1 (MQ=255)
                           gTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGATTCCCGACCGATTTCGTAACGCGGCTGCGCGATg                                                                                                                        >  1:48625/1‑149 (MQ=255)
                                      ggTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGt                                                                                                                   >  2:266904/1‑143 (MQ=255)
                                          aCGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGa                                                                                                         <  1:393389/149‑1 (MQ=255)
                                                 tgtTGATCCCCGGATGATAAGTTCGCAGTCCTTTAAACGAGGGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACaaa                                                                                                  <  1:231510/149‑1 (MQ=255)
                                                     gATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTg                                                                                              >  1:240627/1‑149 (MQ=255)
                                                          ccGGATGATACTTTCGTCGTCCATTACACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATATCAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAAtt                                                                                               <  2:262184/143‑1 (MQ=255)
                                                            ggttgctAAGTGCGCAGTCCATTAAACGAGAGCCACTCCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATTGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGc                                                                                             <  2:138758/137‑1 (MQ=255)
                                                             gatgatAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGt                                                                                      >  1:519508/1‑149 (MQ=255)
                                                                     gTTCGGCGTCCATTAAACGAGGGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTa                                                                              <  1:12026/149‑1 (MQ=255)
                                                                         gCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGGCAGGGAGATGt                                                                                >  2:302892/1‑143 (MQ=255)
                                                                           aGTCCATTAAACGAGAGCCACTGCCAACGTGTTGACCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAACTTGCGTCAGGTCGATGTTa                                                                              >  2:213469/1‑143 (MQ=255)
                                                                             tCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCGATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAAcc                                                                      >  1:351411/1‑149 (MQ=255)
                                                                                aTTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGAt                                                                   >  1:510539/1‑149 (MQ=255)
                                                                                   aaaCGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGGttct                                                                >  1:226534/1‑147 (MQ=255)
                                                                                              agtGCCAACGTGTTGCCCCTGCCTTTGATCAAGCAACAACAGCATAGCTTCCCGACCGATTTCGCAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCtt                                                     <  1:481885/147‑1 (MQ=255)
                                                                                                      cGTGTTGCCCCTGCATTTGATCAAGCAAGAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACt                                             <  1:49322/149‑1 (MQ=255)
                                                                                                        tgtTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCgg                                                 <  2:317860/143‑1 (MQ=255)
                                                                                                                         aTCAAGCAAGAACAGCATAGCTTCCCGACCGGTTTCGCAACGCGGCTGCGCGATGGTTGCCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTGCCGGGACTTTCAGCCCCTGGc                                <  2:171785/143‑1 (MQ=255)
                                                                                                                          tCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGc                         <  1:445998/149‑1 (MQ=255)
                                                                                                                          tCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGc                         <  1:421865/149‑1 (MQ=255)
                                                                                                                             aGCAATAACAGCATAGCTTCCCGACCGATTGCGTAACGCGGCTGCGTGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGttt                            <  2:257612/143‑1 (MQ=255)
                                                                                                                               cAATAACGGCATAGCTTGCAGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAACCCGATTATGGAAAGGTCTTCCGGGCCTTTCAGCCCCTGGCGTTTTg                          >  2:462520/1‑143 (MQ=255)
                                                                                                                               cAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTg                          >  2:115018/1‑143 (MQ=255)
                                                                                                                                aaTAACTGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGc                         <  2:341201/143‑1 (MQ=255)
                                                                                                                                aaTAACAGCCTAGCGTCTCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGc                         <  2:48784/143‑1 (MQ=255)
                                                                                                                                aaTAACAGCAAAGCTTCTCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGc                         <  2:48780/143‑1 (MQ=255)
                                                                                                                                    aCAGCAGAGCTCCCCGACCGATTGCGGAACGCGGCTGCGCGCTGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGt               <  1:170250/149‑1 (MQ=255)
                                                                                                                                                cccGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTAGCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGcc   <  1:119865/149‑1 (MQ=255)
                                                                                                                                                 ccgaccgaTTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTCTTTCCGGAGAAACCgcgctttccgcca  >  1:515495/1‑140 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCCGGATGATAAGTTCGCAGTCCATTAAACGAGAGCCACTGCCAACGTGTTGCCCCTGCATTTGATCAAGCAATAACAGCATAGCTTCCCGACCGATTTCGTAACGCGGCTGCGCGATGGTTGTCAGCGGCGGATCACAAAATTGCGTCAGGTCGATGTTATCAAAACCGATTATGGAAAGGTCTTCCGGGACTTTCAGCCCCTGGCGTTTTGCCTGAGAAAGTGCGCCGAGCGCCA  >  CP009273/4113325‑4113618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑