Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 50.8% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)10 (0.350) 9/270 NT 39.3% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 15 (0.540)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)21 (0.730) 18/270 NT 57.6% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 15 (0.540)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

ACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCC  >  CP009273/2400112‑2400405
                                                                                                                                                    |                                                                                                                                                 
aCTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAAGCCGGACGAAAGTAAAAATGCGAATGCGTTGCACTaa                                                                                                                                                   >  1:444420/1‑149 (MQ=255)
 cTTATCAGATATTATTACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAg                                                                                                                                                  >  1:171249‑M2/1‑148 (MQ=255)
         atatTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAATATGCATATGAGTTGCACTAAggaaggtgc                                                                                                                                          >  1:320591‑M2/1‑140 (MQ=255)
                  cTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTca                                                                                                                                       >  2:381386/1‑143 (MQ=255)
                            gcgcATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacacgtccctg                                                                                                                             <  2:439061‑M2/143‑23 (MQ=255)
                            gcTCATACTGAGCTGGTTAATGCTGTGCATACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgggaacaagtccctgatatga                                                                                                                       <  1:323672‑M2/149‑29 (MQ=255)
                            gcTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatga                                                                                                                       <  1:262946‑M2/149‑29 (MQ=255)
                            gaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCg                                                                                                                             <  2:291316‑M2/26‑1 (MQ=255)
                              tCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgaga                                                                                                                     <  1:91957‑M2/149‑31 (MQ=255)
                                 ggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacctgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCAACTCACATTCTTCCGTtata                                                                                                                        >  2:201393‑M2/113‑143 (MQ=255)
                                  aCTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatga                                                                                                                       >  2:474386‑M2/1‑115 (MQ=255)
                                        cTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgt                                                                                                           <  1:118182‑M2/149‑41 (MQ=255)
                                        cTGGTTAATGCGGTGCACAAAAAAACAAGCAATTCCATTTGGAATATTCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGGTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAg                                                                                                           <  1:408750/149‑1 (MQ=255)
                                           gTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAAGGCACATGAGTTGCACAAAggaaggtgcgaacaagtccctgatatgagaacatgtt                                                                                                              <  2:144841‑M2/143‑38 (MQ=255)
                                           gTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCACTGGCAATTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCAAAATGATCCGTTATAACGCCAGAAg                                                                                                              >  2:342358/1‑143 (MQ=255)
                                            ttAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATca                                                                                                       <  1:428300/149‑1 (MQ=255)
                                             tAATGCATTGAACACACAAACAAGCAATTCCATTGCAAATGTCCCTCCCTGACAGTTTCAGCCGGAACCCAAAAAAAAAAAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTa                                                                                                            <  2:108810/143‑1 (MQ=255)
                                                tGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGAt                                                                                                         >  2:316876/1‑143 (MQ=255)
                                                 tccaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttcccctcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGaa                                                                                                  <  1:395908‑M2/53‑1 (MQ=255)
                                                      ggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAatatat                                                                                             <  1:316271‑M2/58‑1 (MQ=255)
                                                       cacacacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCAGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatag                                                                                            <  1:481762‑M2/149‑56 (MQ=255)
                                                            acaaacaaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAg                                                                                       >  1:212678/1‑149 (MQ=255)
                                                                 caaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAGAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttc                                                                                  <  1:355469‑M2/149‑66 (MQ=255)
                                                                  aaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacaggggtca                                                                                 <  1:174437‑M2/149‑67 (MQ=255)
                                                                    gCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCCTCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTACggaaggtgcgaacaagcccctgttatgagatcatgtttgtcatctggatccatagaacaggg                                                                                     >  2:286887‑M2/1‑81 (MQ=255)
                                                                    gCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacaggg                                                                                     >  2:146966‑M2/1‑81 (MQ=255)
                                                                    gCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaaggccctgatatgagatcatgtttgtcatctggagccatagaacaggg                                                                                     <  2:156456‑M2/143‑63 (MQ=255)
                                                                      aaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGAt                                                                                   >  2:293261‑M2/76‑143 (MQ=255)
                                                                         tCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCt                                                                          <  1:235315/149‑1 (MQ=255)
                                                                          tcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCa                                                                               <  2:138978‑M2/72‑1 (MQ=255)
                                                                            aTTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacaggggtcatcatgagtca                                                                       <  1:197666‑M2/149‑77 (MQ=255)
                                                                                   aTGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATACATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatcgaacagggttcctcatgagtcatcaaatt                                                                <  1:18096‑M2/149‑84 (MQ=255)
                                                                                    tgtgCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggcgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatc                                                                     <  2:368545‑M2/143‑79 (MQ=255)
                                                                                         cTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTactcactc                                                          >  1:9905/1‑149 (MQ=255)
                                                                                         cTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACCCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTac                                                                >  2:1742/1‑143 (MQ=255)
                                                                                          taaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTact                                                               <  2:272745‑M2/88‑1 (MQ=255)
                                                                                            aatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCCCAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCttt                                                       >  1:429011‑M2/54‑149 (MQ=255)
                                                                                                   ttacggagcaagccgacaagacttcccccggtttttcggaccgtccCTAAAAAAGCGACTCTCATTGTTCCGTTATTATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCtttt                                                      <  2:450206‑M2/97‑1 (MQ=255)
                                                                                                   tgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCtttt                                                      <  2:450351‑M2/97‑1 (MQ=255)
                                                                                                          ccGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTATATAAGCGACTCACATTGTTCCTTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGTTCGCATATCTAGTAAGTTACTCACTCTTTTCTATTTa                                               <  2:187864/143‑1 (MQ=255)
                                                                                                           cGGACCCCGGACGAAAGTAAAAATGCATAGTAGTTGCACCAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaa                                              >  2:289924‑M2/1‑42 (MQ=255)
                                                                                                                cccGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATCTATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgt                                   <  1:222387/149‑1 (MQ=255)
                                                                                                                    gACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGttt                               <  1:355965/149‑1 (MQ=255)
                                                                                                                       gAAAGTAAAAATGCATATGAGTTGCACTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaag                                  <  2:397818‑M2/143‑114 (MQ=255)
                                                                                                                                      tatGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaa                   <  2:8528/143‑1 (MQ=255)
                                                                                                                                      taagAGCTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGACCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa             <  1:134106/146‑1 (MQ=255)
                                                                                                                                       atGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagaga            >  1:226410‑M2/1‑14 (MQ=255)
                                                                                                                                          aGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatgatgaggcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaag               >  2:19847‑M2/1‑11 (MQ=255)
                                                                                                                                                 cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgccagtgaattcagcagtaagcggcgtcagaccagaaaagagattttcttgtc  <  1:180020‑M2/149‑146 (MQ=255)
                                                                                                                                                 cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagcccagaaaagagatttt        <  2:28283‑M2/143‑140 (MQ=255)
                                                                                                                                                 cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagatttt        <  2:7164‑M2/143‑140 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCC  >  CP009273/2400112‑2400405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.