Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	2,420,338:1	+AATT	51.8%	intergenic (‑71/+150)	hisJ ← / ← argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 2420334	17 (0.610)	18 (0.650) +AATT	16/254	NT	51.8%	intergenic (‑67/+154)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
	?	CP009273	2420335 =	17 (0.590)					intergenic (‑68/+153)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACA  >  CP009273/2420188‑2420471                                                                                                                                                   |                                                                                                                                          ttttGCGGAAACACATCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGATCCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaa                                                                                                                                         <  1:139308‑M1/149‑3 (MQ=255)              cagcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGGTAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAGTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatc                                                                                                                            >  1:275081‑M1/1‑134 (MQ=255)                gcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaa                                                                                                                          <  1:314357‑M1/149‑18 (MQ=255)                   aaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCCCCAGTTGTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGcgaca                                                                                                                       >  1:79105/1‑145 (MQ=255)                      gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATCATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGCTTGCTACACTTATCGTAAAATAAaattaattgaccatcga                                                                                                                          >  2:449931‑M1/1‑126 (MQ=255)                      gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgagcatcaa                                                                                                                          >  2:124444‑M1/1‑126 (MQ=255)                        cgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACCCTTATCGTGCCATAAAATTGCCCATCAAGGCaca                                                                                                                        >  2:390491/1‑141 (MQ=255)                                cgcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgcc                                                                                                          >  1:305916‑M1/1‑116 (MQ=255)                                 gcTGGGAAAGGTCAGCACCAGAGAGAGCTATACCACCAGTTTTTGCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattaaccatcaaggcaaaatacc                                                                                                               <  2:411349‑M1/143‑29 (MQ=255)                                  cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGcc                                                                                                              >  2:444633/1‑143 (MQ=255)                                   tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                                                                                                       >  1:406440/1‑149 (MQ=255)                                    ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGa                                                                                                      >  1:413540/1‑149 (MQ=255)                                    ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgt                                                                                                      >  1:525408‑M1/1‑112 (MQ=255)                                         aGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCTACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtat                                                                                                 <  1:30790/149‑1 (MQ=255)                                                 aCCAGAGAGAGCGATATCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatt                                                                                         <  1:78480‑M1/149‑51 (MQ=255)                                                  ccAGAGAGAGCGTTAGCACCAGTTTTTTCTTTATGTCCTCAACTGGCAGTCTTTTTATAACGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattattt                                                                                        <  1:259877‑M1/149‑52 (MQ=255)                                                  ccAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtattat                                                                                              >  2:86711/1‑143 (MQ=255)                                                        agagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa                                                                                        <  2:17882/143‑1 (MQ=255)                                                        agacCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTACCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa                                                                                        <  2:339781/143‑1 (MQ=255)                                                                aGCACCATTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTaa                                                                          <  1:397120/149‑1 (MQ=255)                                                                              cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTCTGCATTTGAAt                                                            >  1:129427/1‑149 (MQ=255)                                                                              cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCACAAAATTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATACTTAAGTATGCATTTGAAt                                                            >  1:318997/1‑149 (MQ=255)                                                                                     tCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAACGCTTAAGTATGCATTTAAATg                                                           >  2:217060/1‑143 (MQ=255)                                                                                              gACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcgaaatatcccgccccgttagacgtattatttttcattaaacatgcttacgtatgaatttccatgcccaa                                                  >  2:129255‑M1/1‑54 (MQ=255)                                                                                                         tataGGGAATATTTACAGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatcgtgcaa                                       >  2:454441‑M1/1‑43 (MQ=255)                                                                                                              ggAATATTTACAGGTTAGTTGCACTTATCGTAACATAAaattaattgaccatcaaggcaaagtatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaac                                  >  2:333787‑M1/1‑38 (MQ=255)                                                                                                               gAATATTTACAGGTTTGCTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGc                                 <  2:294257/143‑1 (MQ=255)                                                                                                                    tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa                            >  2:377532/1‑143 (MQ=255)                                                                                                                        cAGGTTTGTGACCCTTATCGTAACATAAaattaatagaccatcaaggcaaaatctcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatga                  <  1:397017‑M1/149‑122 (MQ=255)                                                                                                                         aGGTTTGTTACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttctttaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctgccgtgcaccatgat                 <  1:361877‑M1/149‑123 (MQ=255)                                                                                                                           gTTTCTCACACTTATCGTAACATAAaattaatggcccatcaaggcaaaatatcccgccccgttagacgtattatttttcattacaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcacca                     <  2:426756‑M1/143‑119 (MQ=255)                                                                                                                            tttGTTACACTTATCAGGCCATAAAAGTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAtt              <  1:302645/149‑1 (MQ=255)                                                                                                                                ttACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatg                <  2:270891‑M1/143‑124 (MQ=255)                                                                                                                                 tACACTTATCGTAACATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatga               <  2:475695‑M1/143‑125 (MQ=255)                                                                                                                                      ttATCGTAATATAAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatcatt          <  2:213782‑M1/143‑130 (MQ=255)                                                                                                                                        ctCGTGCCATAAATATTACCATCAAGGCAAAATTTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATCCATTTCAATGCACTACGATAGTGCAACAAACCTGGCGAGCACCATGATGATCATTCCATCAGGTACa  <  1:322059/148‑1 (MQ=255)                                                                                                                                            taacataAaattaattgaccatcaaggcaaaatatcccgccccgttagacgtattatttttcattaaaaatgcttaagtatgcatttcaatgcaccacgatagtgcaacaaacctggcgtgcaccatgatgatccttccatca    >  2:197448‑M1/4‑8 (MQ=255)                                                                                                                                                   |                                                                                                                                          TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACA  >  CP009273/2420188‑2420471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.