Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,138,811 T→C D338G (GAC→GGC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,138,8110TC98.5% 202.8 / NA 66D338G (GAC→GGC) rnefused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (30/34);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGGA  >  CP009273/1138665‑1138956
                                                                                                                                                  |                                                                                                                                                 
tttCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCa                                                                                                                                                 >  2:950835/1‑149 (MQ=255)
       gaggcacacgCGAGAAATATGGCTGATTTGAATACGCTCACGGTCCTGACGCGCCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGACGTCGATAACAATCAGGCCGCCGAGGCCACGCAGgc                                                                                                                                          <  1:645003/140‑1 (MQ=255)
       cagGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGgc                                                                                                                                          <  2:763492/149‑1 (MQ=255)
          gCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCa                                                                                                                                       >  1:231320/1‑149 (MQ=255)
           ccAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAg                                                                                                                                      >  1:717127/1‑149 (MQ=255)
                  gagaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGGACCGCTTCACGCAGACTGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGa                                                                                                                               <  1:191060/149‑1 (MQ=255)
                  gagaAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGa                                                                                                                               <  2:727686/149‑1 (MQ=255)
                     aaaTATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGATTTCTACCGCCCGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGAAAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGCCGAGCa                                                                                                                            >  2:646894/1‑149 (MQ=255)
                        tacggCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAAtc                                                                                                                         <  1:62520/146‑1 (MQ=255)
                             cTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGTCGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATc                                                                                                                    <  2:242413/149‑1 (MQ=255)
                             cTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATc                                                                                                                    <  2:506551/149‑1 (MQ=255)
                              tGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCg                                                                                                                   <  1:265013/149‑1 (MQ=255)
                                aTTGGAACACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACGGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGc                                                                                                                 <  2:945516/149‑1 (MQ=255)
                                     aaTACGCGCACGGTCCTGACGCACCGATTGACACTGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCtt                                                                                                            >  1:479154/1‑149 (MQ=255)
                                        aCGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGa                                                                                                         <  2:1033944/149‑1 (MQ=255)
                                         cgcgCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAg                                                                                                        <  2:231320/149‑1 (MQ=255)
                                          gcgcACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCGCATCGGCGGGTTCGAgg                                                                                                       >  1:580488/1‑149 (MQ=255)
                                               cGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAgt                                                                                                  <  2:65033/149‑1 (MQ=255)
                                                 gTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGCTAgggt                                                                                                >  1:79140/1‑149 (MQ=255)
                                                    cTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTac                                                                                             <  1:182313/149‑2 (MQ=255)
                                                     tGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCCGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTaaa                                                                                            <  1:531046/149‑1 (MQ=255)
                                                      gACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGAAGAGCAATCTCATCGGCAGCTTCGCGGTTGGTGTTCAAc                                                                                           >  1:463921/1‑149 (MQ=255)
                                                        cGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgc                                                                                         <  1:20890/149‑1 (MQ=255)
                                                         gCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgcg                                                                                        <  1:917533/149‑1 (MQ=255)
                                                         gCACCGCTTCACCCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAAcgcg                                                                                        <  1:904112/149‑1 (MQ=255)
                                                          cACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCgg                                                                                       <  1:950835/149‑1 (MQ=255)
                                                           aCCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGt                                                                                      >  2:99800/1‑149 (MQ=255)
                                                              gCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttc                                                                                   >  2:836247/1‑149 (MQ=255)
                                                               cTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGTGTCATTTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttct                                                                                  <  2:128600/149‑1 (MQ=255)
                                                               cTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTCGTGTTAAACGCGGTctct                                                                                  >  1:588298/1‑149 (MQ=255)
                                                               cTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttct                                                                                  >  1:1008693/1‑149 (MQ=255)
                                                                ttCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCTTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttctt                                                                                 <  2:14175/149‑1 (MQ=255)
                                                                 tCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTttcttc                                                                                <  1:747061/149‑1 (MQ=255)
                                                                   aCGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGa                                                                              >  2:817024/1‑149 (MQ=255)
                                                                     ggaGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGata                                                                            <  1:526265/147‑1 (MQ=255)
                                                                     gCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGata                                                                            >  2:551028/1‑149 (MQ=255)
                                                                             ttttCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgccgcc                                                                    >  1:298282/1‑149 (MQ=255)
                                                                               ttCTACCGCACGCTGGTGGCGTACTGGCGTCATGGCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATcgccgccgc                                                                  >  2:521342/1‑149 (MQ=255)
                                                                               ttCTACCGCACGCTGGTGGCGTACTGGCGCCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATACCAGCGACAGATTCGAGGTTAGTGTTAAAAGCGGTTTCTTCGATATcgccgccgc                                                                  >  2:44524/1‑149 (MQ=255)
                                                                                  tACCGCACGCTGGTGGCGTACTGGCGTCATGTAGCTGTACTCGATTACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCggg                                                               >  2:1023688/1‑149 (MQ=255)
                                                                                  aacgcaccgtgggtggCG‑ACCGGCGTCATGTCGATGGAGTCGATAACCATCCGGCCGGCGGGGCCACGGAGGCGGCGCCGACGAGCAATCCCATCGGCAGGTTCGGGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGt                                                              <  1:1027145/138‑1 (MQ=255)
                                                                                          gCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGGGGTTAGGGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGt                                                       >  1:902171/1‑149 (MQ=255)
                                                                                           cTGGTGCCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTg                                                      <  2:843431/149‑1 (MQ=255)
                                                                                              gtggCGTACTGGCGGCGTGTCGATGAAGTCGATAACAATGCGGCGGCGGAGGCCACGCAGGCGCATTTGACGAGCAATCGCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCgg                                                   <  2:268130/149‑1 (MQ=255)
                                                                                                ggCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGACGAAGCTGCCACTCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAg                                                 <  2:79140/149‑1 (MQ=255)
                                                                                                               tGTCGATGTAGTTGATAACTTTCGGGCCGCCGAGGTCACGCTGGCGCAGTTGTCGTGTTATTTGTTCGGGAGCTTGGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGcc                                  <  2:375581/149‑1 (MQ=255)
                                                                                                                gTCGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAACCTCATCGGCAGCTTCGAGGGTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGTGTCGCGCGTGCGGAGTTGATGTCGATGGCCg                                 >  1:758174/1‑149 (MQ=255)
                                                                                                                  cGATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGtt                               <  1:99800/149‑1 (MQ=255)
                                                                                                                   gATGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTGGATGGCCGTTa                              >  1:864017/1‑149 (MQ=255)
                                                                                                                    aTGAAGTCGATTCCAATCAGGGCGCCGAGGCTACGGAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTaa                             <  2:497904/149‑1 (MQ=255)
                                                                                                                    aTGAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTaa                             <  2:479154/149‑1 (MQ=255)
                                                                                                                      gAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGAGGTCGATGGCCGTTCACg                           >  1:299520/1‑149 (MQ=255)
                                                                                                                      gAAGTCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTAACGAGCAATCTCATCGGCAGCTTCGAGCTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTCCGGAGATGATGTCGATGGCCGGGAACg                           >  2:775069/1‑149 (MQ=255)
                                                                                                                          tCGATAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGCTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTGACGCGTc                       >  1:910751/1‑149 (MQ=255)
                                                                                                                             aTAACAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGt                    >  2:383525/1‑149 (MQ=255)
                                                                                                                              tAACACTCCGGCCGCCGAGGCCACGCAGGCGCAGCTCACGAGCAATCTCCTCGGCAGCTTCGAGGTTAGTGTTAACCGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTg                   <  1:536549/149‑1 (MQ=255)
                                                                                                                              gaaCAATCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTTATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTg                   <  2:60039/148‑1 (MQ=255)
                                                                                                                                  aaTCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGCTGGTGTCGATGTCAGTTAACGCTTCGGTGcgat               >  2:696320/1‑146 (MQ=255)
                                                                                                                                   aTCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGGTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTc              >  2:821310/1‑149 (MQ=255)
                                                                                                                                   aTCAGGCCGCCGAGGACACGCAGGCGCAACGGACGAGCAATCTCACTGGCTGCTCCGTGGTGAGGGTTAAACGCTCTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTc              <  1:975315/149‑1 (MQ=255)
                                                                                                                                    tCAGGCCGCCGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCCGTGCTGTCg             >  2:620338/1‑149 (MQ=255)
                                                                                                                                       ggCCGCCGAGGCCACGCACGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATa          >  2:759270/1‑149 (MQ=255)
                                                                                                                                        gccgccGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATaa         <  2:801585/149‑1 (MQ=255)
                                                                                                                                        gccgccGAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATaa         <  2:229199/149‑1 (MQ=255)
                                                                                                                                              gAGGCCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTACAAGGTTAGTGTTAAACGCGGTTGCTTCGATATCGCCGCCGCGAGTCGCGCGTGCGGAGTTGATGTCGGTGGCCGTTAACGCTTCGGGGCTGTCGATAACaagac   >  1:501352/1‑146 (MQ=255)
                                                                                                                                               aGGCCAGGCAGGCGCAGTTGTCTAGGAATCTCATCGGCAGCTTGGAGGTTAGTGTTAATCGCGGTTTCTTCGATATCGCCGCCCCCGGTCGCCCGTGCGGAGGTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGGa  <  1:777452/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTCACGCAGACGGTTTTCTACCGCACGCTGGTGGCGTACTGGCGTCATGTCGATGAAGTCGATAACAATCAGGCCGCCGAGGTCACGCAGGCGCAGCTGACGAGCAATCTCATCGGCAGCTTCGAGGTTAGTGTTAAACGCGGTTTCTTCGATATCGCCGCCGCGGGTCGCGCGTGCGGAGTTGATGTCGATGGCCGTTAACGCTTCGGTGCTGTCGATAACAATGGA  >  CP009273/1138665‑1138956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑