| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,419,933 | G→T | 66.4% | A112D (GCC→GAC) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,419,933 | 0 | G | T | 66.4% | 7.8 / 2.6 | 11 | A112D (GCC→GAC) | hisJ | histidine/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (4/3); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACAT > CP009273/2419812‑2420070 | ccAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGGCAGAATTTTTCGCCACCACCAACCGAGAATGGTCAGCGCACGGTTGATCGGTGAGGGCttt > 2:297358/1‑148 (MQ=255) gTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCtt < 2:346072/150‑1 (MQ=255) ggtggtGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGa > 2:151550/1‑150 (MQ=255) tgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGCCTGAATGTCAGAATTTTTCGCCACCCACAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGCAGGCTATTTCTTGCCGACGTTTTTCGGTTATGGtaaagg > 1:235909/1‑147 (MQ=255) ccTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATg < 2:195282/150‑1 (MQ=255) aaTACGCCTACCTGTTTGCCTTTCAGCGACTCGGCTGTCCGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGTCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACatg < 1:26895/150‑1 (MQ=255) tcTGAATGTCGTAAGTTTTCGCCACCACCAAACGACAATCGGCAGCGTACAGTTTGGCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACg < 1:49603/149‑1 (MQ=255) aTGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATcc > 2:304496/1‑150 (MQ=255) ttCGCCACCACCAAACGAGAAAAAGCAGCGTACAGTCAGTCGGTGAAGGCTATTTCATGCTACCGTTTTAACGTACAGGAAAGCGACGACATCGTGGCGTCAAGCATCCTCCCTTTTAAGGACGGGATTAACGAATCCCGCgggcttcgc > 1:101324/1‑143 (MQ=255) tCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa < 2:207420/150‑1 (MQ=255) aaaCGAGAATCGTCAGCGTACAGTTTGTCGGTGAAAGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGAACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTAcct > 2:180646/1‑148 (MQ=255) | CCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACAT > CP009273/2419812‑2420070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |