| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,378,108:1 | +C | 72.2% | coding (47/471 nt) | argR → | l‑arginine‑responsive arginine metabolism regulon transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,378,107 | 1 | . | C | 72.2% | 35.8 / 16.5 | 18 | coding (46/471 nt) | argR | l‑arginine‑responsive arginine metabolism regulon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base C (8/5); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
ATTATTTCAGCCTTCTTCAGGGCTGACTGTTTGCATAAAAATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAG‑CATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAAAATGGAAATG > CP009273/3377971‑3378256 | attattTCAGCCTTCTTCAGGGCTGACTGTTTGCATAAAAATTCATCTGTTTGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGACGCTCGGCTAAGAAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTaatg > 1:233860/1‑148 (MQ=255) attCCAGCCTCCTTCCGGGCAGACTGTTTGCATAAAAATTCATCTTTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAaagaag < 2:115971/150‑1 (MQ=255) cttcAGGGCTGACTGTTTGCATAAAAATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGc < 1:353290/150‑1 (MQ=255) ggCTGACTGTTTGCATAAAAATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAg < 1:331165/150‑1 (MQ=255) gCTGACTGTTTGCATAAAAATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAG‑CATTACTTAAAGAAGAGAAATTTAGCTCCCAggg < 2:356714/150‑1 (MQ=255) tGTTTGCATAAACATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCACTGAAAG‑CATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAAt < 1:277443/150‑1 (MQ=255) gTTTGCATAAAAATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGCGCGAAAt > 2:94871/1‑150 (MQ=255) cTGTATGCCCAATACTTCTGTATCACCCTCCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAG‑CATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAg < 2:289602/150‑1 (MQ=255) cTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGGAATTTAGCTCCCAGGGCGAACTCGTCGACGCGTTGCAGGa > 2:16840/1‑150 (MQ=255) tGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCAGTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCCggcg > 2:26517/1‑148 (MQ=255) tCAACCACCATATCGGGTGACTTTTGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTACAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACCATATTAAt > 1:154208/1‑150 (MQ=255) cGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAG‑CATTACTTCAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCGGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGtctc > 2:335241/1‑150 (MQ=255) ggTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTcg > 1:155764/1‑150 (MQ=255) cTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCggct > 1:177062/1‑148 (MQ=255) tAAGCAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTg > 2:1412/1‑150 (MQ=255) cAAGAAGAACTAGTTAAAGCATTTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTgt < 2:302210/150‑1 (MQ=255) gaagaaCTAGTGAAAGCATGTAAAGCCATTACTTAAAGAAGAGAAATTTAGCTCGCAGGGCGAAATCGTCGCTGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTgtacg < 1:35764/150‑1 (MQ=255) aaCTAGTTAAAGCATTTAAAG‑CATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTacac > 1:205799/1‑150 (MQ=255) natttaaagccatTACTAAAAGAAGAGAAAGTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGCCCAAGTTTGGTGCTGTACGTACACGCAATGCCaaa > 2:224983/2‑150 (MQ=255) g‑cattACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAaaatggaaatg > 2:336918/1‑150 (MQ=255) | ATTATTTCAGCCTTCTTCAGGGCTGACTGTTTGCATAAAAATTCATCTGTATGCACAATAATGTTGTATCAACCACCATATCGGGTGACTTATGCGAAGCTCGGCTAAGCAAGAAGAACTAGTTAAAGCATTTAAAG‑CATTACTTAAAGAAGAGAAATTTAGCTCCCAGGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAAAATGGAAATG > CP009273/3377971‑3378256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |